Use of HPLC- and GC-QTOF to determine hydrophilic and lipophilic phenols in mango fruit (Mangifera indica L.) and its by-products

López-Cobo, Ana; Verardo, Vito; Díaz‐de‐Cerio, Elixabet; Segura‐Carretero, Antonio; Fernández‐Gutiérrez, Alberto; Gómez-Caravaca, Ana María

doi:10.1016/j.foodres.2017.02.008

Cited by 98 publications

(70 citation statements)

References 40 publications

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“…The first peak (15.9 min) was identified as cyanidin 3-D-galactoside according to standard. The second peak (22.1 min) was identified as 7-O-methylcyanidin 3-O-b-D-galactopyranoside based on retention time, spectrum and mango literature (Berardini et al, 2005a,b;Lopez-Cobo et al, 2017) (Fig. 2b).…”

Section: Hplc Analysis Of Red and Green Mango Peel Extractsmentioning

confidence: 99%

Glycosylated flavonoids: fruit's concealed antifungal arsenal

et al. 2019

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Fruit defense against pathogens relies on induced and preformed mechanisms. The present contribution evaluated performed resistance of red and green mango fruit against the fungal pathogen Colletotrichum gloeosporioides and identified the main active antifungal components.High-performance liquid chromatography analysis of nonhydrolyzed mango peel extracts identified major anthocyanin peaks of glycosylated cyanidin and methylcyanidin, and flavonol peaks of glycosylated quercetin and kaempferol, which were more abundant on the 'red side' of red mango fruit. Organic extracts of red vs green mango peel were more efficient in inhibiting C. gloeosporioides.Transcriptome analysis of the mango-C. gloeosporioides interaction showed increased expression of glucosidase genes related to both fungal pathogenicity and host defense. Glucosidase treatment of organic peel extract increased its antifungal activity. Additionally, quercetin and cyanidin had significantly higher antifungal activity than their glycosylated derivatives. Peel extract volatiles treated with glucosidase had antifungal activity. GCMS analysis identified 15 volatiles after glucosidase hydrolysis, seven of them present only in red fruit.These results suggest that the fruit obtains a concealed arsenal of glycosylated flavonoids in its peel when they are hydrolyzed by b-glucosidase that is induced in both fungus and host during infection process, become more toxic to the fungal pathogen, inhibiting decay development.

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Section: Hplc Analysis Of Red and Green Mango Peel Extractsmentioning

confidence: 99%

Glycosylated flavonoids: fruit's concealed antifungal arsenal

et al. 2019

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“…In a recent study of López‐Cobo et al . (), ellagic acid was also detected as one of the main phenolic compounds in mango seed. This compound was more dominant in the soluble fraction than in the bound phenolic fraction.…”

Section: Resultsmentioning

confidence: 92%

In vitro antioxidant activity and phenolic profiles of tropical fruit by‐products

Nguyen

Vissenaekens

et al. 2019

Int J of Food Sci Tech

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Peel and seeds of red-skinned passion fruit, mango, longan, rambutan, white-flesh and red-flesh dragon fruit were screened for their in vitro antioxidant activity, and determination of a detailed profile of phenolic compounds. Rambutan peel and mango seed extracts exhibited the highest total phenolic content and antioxidant activities (DPPH, ABTS and FRAP values). By using UPLC-QTOF-MS/MS analysis, the profiles of soluble and bound phenolics in the fruit by-products were obtained. Ellagic acid, geraniin, quercetin hexoside, gallic and galloyshikimic acid were predominant in rambutan peel, whereas, mangiferin, ellagic acid and galloy(di)glucoside were the major phenolic compounds in mango seed. Main phenolic compounds in longan peel were ellagic acid, galloyldiglucoside, and gallic acid, while in dragon fruit peel this was isorhamnetin glycoside, isorhamnetin glucorhamoside. Meanwhile, rutin and quercetin hexoside were predominant in passion fruit peel. These findings contribute significantly to the database of phenolic profiles of by-products of tropical fruits.

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“…The ABTS radical scavenging capacity of Keitt peel has already been reported with a value of 380 ± 4 mg AAE/g d.w. while using different solvent concentration [15]. López-Cobo, et al [17] also reported the ABTS value of Keitt peel, which is 475.4 ± 8.7 µM FeSO 4 /mg d.w. using iron sulphate (FeSO 4 ) as the standard.…”

Section: Antioxidant Activities (Dpph Frap and Abts)mentioning

confidence: 90%

“…Meneses, et al [33] extracted the polyphenols from mango by-product (mainly mang peel) while using supercritical fluid extraction technique and reported the concentrations of quercetin, quercetin-3-galactoside, and kaempferol in the range of 0.48-0.61, 9.96-11.49, and 0.08-0.11, respectively. Previously, López-Cobo et al [17] also reported polyphenols from Keitt mango peel, including gallic acid (0.12 mg/g d.w. ), syringic acid (0.07 mg/g d.w. ), catechin (0.11 mg/g d.w ), and quercetin-3-galactoside (0.16 mg/g d.w. ), extracted with 80% methanol extract. Ribeiro, et al [34] measured quercetin, quercetin-3-galactoside, and kaempferol-3-glucoside content in Ubá mango peel, which is slightly lower than our reported values.…”

Section: Quantitative Determination Of Polyphenols By Hplc-pdamentioning

confidence: 94%

LC-ESI-QTOF/MS Profiling of Australian Mango Peel By-Product Polyphenols and Their Potential Antioxidant Activities

et al. 2019

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Mango (Mangifera indica L.) is one of the most important fruits in the world. Mango peel is an important by-product that is rich in polyphenols and it could have high economic value if it is effectively utilized. Phenolic characterization is an essential step in the commercial utilization of mango peel by-products as food ingredients. Herein, qualitative and quantitative analyses of two Australian mango peel “Keitt” and “Kensington Pride” (K&P) by-products were conducted while using liquid chromatography coupled to electrospray ionisation and quadrupole time of flight mass spectrometry (LC-ESI-QTOF/MS) and high-performance liquid chromatography coupled to photodiode array detector (HPLC-PDA). A total of 98 polyphenols compounds were tentatively identified in both Keitt peel and K&P peel extracts, with greater concentrations of these compounds being detected in Keitt peel. The total phenolic content (TPC), total flavonoid content (TFC), and a total tannin content (TTC) were determined. The antioxidant activity of mango peel by-products was determined while using 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Keitt peel contained higher concentrations of total phenolic compounds, flavonoids, and tannins and had higher antioxidant capacity in DPPH, FRAP, and ABTS assays as compared to K&P peel. In HPLC-PDA quantification, the predominant phenolic compounds in Keitt peel and K&P peel were catechin (62.32 ± 0.01 mg/gd.w.) and syringic acid (17.78 ± 0.01 mg/gd.w).

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Use of HPLC- and GC-QTOF to determine hydrophilic and lipophilic phenols in mango fruit (Mangifera indica L.) and its by-products

Cited by 98 publications

References 40 publications

Glycosylated flavonoids: fruit's concealed antifungal arsenal

Glycosylated flavonoids: fruit's concealed antifungal arsenal

In vitro antioxidant activity and phenolic profiles of tropical fruit by‐products

LC-ESI-QTOF/MS Profiling of Australian Mango Peel By-Product Polyphenols and Their Potential Antioxidant Activities

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