Use of hopane as a conservative biomarker for monitoring the bioremediation effectiveness of crude oil contaminating a sandy beach

Venosa, Albert D.; Suidan, M T; King, Dennis W.; Wrenn, Brian A.

doi:10.1038/sj.jim.2900304

Cited by 99 publications

(55 citation statements)

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“…3), but decrease in the TPH contents to the low level achieved during the stage was very difficult. Suitability of procedures in order to elaborate a mathematical model of the TPH biodegradation is confirmed by reports in the literature (Venosa et al 1997;Namkoong et al 2002;Obbard 2003, 2004).…”

Section: Biodegradation Of Petroleum Hydrocarbons From the G-44 Wastesupporting

confidence: 71%

“…The hopane-normalized results of TPH chromatographic tests, carried out during biodegradation of TPH, were used to construct a first-order mathematical model of this process (Venosa et al 1997;Xu and Obbard 2003;). It was found that the following relation holds:…”

Section: Statistical Analysis and Tph First-order Biodegradation Modelmentioning

confidence: 99%

“…It enables the evaluation of biodegradation degree of hydrocarbons. This approach is useful in the formulation of a simplified mathematical model describing TPH biodegradation process Venosa et al 1997;Obbard 2003, 2004). The results of chromatographic analysis and microbiological tests are a basic tool, which can be used to create a biopreparation based on indigenous microorganisms.…”

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confidence: 99%

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Effectiveness of Bioremediation Processes of Hydrocarbon Pollutants in Weathered Drill Wastes

Steliga

Kapusta

Jakubowicz

2009

Water Air Soil Pollut

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The aim of this article is to present the problem of purification of 50-year-old weathered wastes (soil) from waste pits-the result of oil drilling. The soil was deeply contaminated with petroleum hydrocarbons-total petroleum hydrocarbon (TPH) level: 85,654-101,842 mg kg dry mass. This work presents results of waste pit material purification with the use of stage technology: initial reclamation, basic bioremediation, and bioaugmentation (inoculation with indigenous microorganisms). The whole process was controlled with the use of gas chromatography/flame ionization detector. This analytical method enables observation of alternation in nalkanes content during the consecutive stages of purification. According to this method, estimation of oil hydrocarbon biodegradation degree with the use of n-C 17 /Pr and n-C 18 /F indicators can be done. The use of biomarker C 30 -17α(H)21β(H)-hopane to normalize the TPH concentration in laboratory research enabled the creation of the first-order mathematical model of biodegradation. It is possible to recognize the dynamics of the following purification stages due to the calculated first-order biodegradation constants. Decrease in the TPH content (63.8-65.1%) was a result of laboratory tests led in 130 days of basic bioremediation. The next stage of soil purification (130 days) included inoculation with biopreparation based on indigenous microorganisms-TPH decrease in 80.7-81.7%. Laboratory tests results enabled elaboration of purification methods applied in tested waste pits in industrial conditions (in situ). The technology of the G-44 and G-12 waste pits purification from huge petroleum hydrocarbons content, consisting of stage purification process, enables the TPH decrease to the satisfactory level in 3 years.

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Section: Biodegradation Of Petroleum Hydrocarbons From the G-44 Wastesupporting

confidence: 71%

Section: Statistical Analysis and Tph First-order Biodegradation Modelmentioning

confidence: 99%

mentioning

confidence: 99%

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Effectiveness of Bioremediation Processes of Hydrocarbon Pollutants in Weathered Drill Wastes

Steliga

Kapusta

Jakubowicz

2009

Water Air Soil Pollut

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“…To begin our analyses of these compounds, we used hopane as an internal standard, first normalizing each analyte concentration to the hopane concentration in that sample (36,37) and then normalizing that ratio to the corresponding ratio in source oil. We refer to these doubly normalized values as the "fraction remaining" of a given compound.…”

Section: Resultsmentioning

confidence: 99%

Persistence and biodegradation of oil at the ocean floor following Deepwater Horizon

Bagby

Reddy

Aeppli

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The 2010 Deepwater Horizon disaster introduced an unprecedented discharge of oil into the deep Gulf of Mexico. Considerable uncertainty has persisted regarding the oil’s fate and effects in the deep ocean. In this work we assess the compound-specific rates of biodegradation for 125 aliphatic, aromatic, and biomarker petroleum hydrocarbons that settled to the deep ocean floor following release from the damaged Macondo Well. Based on a dataset comprising measurements of up to 168 distinct hydrocarbon analytes in 2,980 sediment samples collected within 4 y of the spill, we develop a Macondo oil “fingerprint” and conservatively identify a subset of 312 surficial samples consistent with contamination by Macondo oil. Three trends emerge from analysis of the biodegradation rates of 125 individual hydrocarbons in these samples. First, molecular structure served to modulate biodegradation in a predictable fashion, with the simplest structures subject to fastest loss, indicating that biodegradation in the deep ocean progresses similarly to other environments. Second, for many alkanes and polycyclic aromatic hydrocarbons biodegradation occurred in two distinct phases, consistent with rapid loss while oil particles remained suspended followed by slow loss after deposition to the seafloor. Third, the extent of biodegradation for any given sample was influenced by the hydrocarbon content, leading to substantially greater hydrocarbon persistence among the more highly contaminated samples. In addition, under some conditions we find strong evidence for extensive degradation of numerous petroleum biomarkers, notably including the native internal standard 17α(H),21β(H)-hopane, commonly used to calculate the extent of oil weathering.

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“…To reduce sample heterogeneity in the crude oil-contaminated soil, samples were extensively mixed prior to placing the soil in the microplots. We also used a biomarker, C 30 17α(H),21β(H)-hopane, as an internal standard to normalize PAH values (Frontera-Suau et al, 2002;Prince et al, 1994;Teal et al, 1992;Venosa et al, 1997). The PAH data were normalized against the corresponding hopane value obtained for that specific sample, to obtain a PAH to hopane ratio.…”

Section: Polycyclic Aromatic Hydrocarbon Levelsmentioning

confidence: 99%

Phytoremediation of Alkylated Polycyclic Aromatic Hydrocarbons in a Crude Oil-Contaminated Soil

White

Wolf

Thoma

et al. 2006

Water Air Soil Pollut

123

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Phytoremediation uses plants and their associated microorganisms in conjunction with agronomic techniques to remove or degrade environmental contaminants. The objective of the field study was to evaluate the effect of vegetation establishment plus fertilizer addition on the biodegradation of alkylated polycyclic aromatic hydrocarbons in a crude oil-contaminated soil. Four replications of the following treatments were used: non-vegetated non-fertilized control; fescue (Lolium arundinaceum Schreb.) − ryegrass (Lolium multiflorum L.) mixture + fertilizer; or bermudagrass (Cynodon dactylon (L.) Pers.) − fescue mixture + fertilizer. Vegetation was successfully established at the site that had an initial total petroleum hydrocarbon (TPH) concentration of 9,175 mg/kg. While alkylated two-ring naphthalenes were degraded in all treatments equally, there was greater degradation of the larger three-ring alkylated phenanthrenes-anthracenes and dibenzothiophenes in the vegetated fertilized plots compared to the non-vegetated non-fertilized plots. In this field study, an increase in rhizosphere soil volume associated with increased root length along with nutrient additions resulted in increased total bacterial, fungal, and polycyclic aromatic hydrocarbon (PAH) degrader numbers that most likely resulted in increased biodegradation of the more recalcitrant alkylated polycyclic aromatic hydrocarbon compounds in the crude oil-contaminated soil.

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Use of hopane as a conservative biomarker for monitoring the bioremediation effectiveness of crude oil contaminating a sandy beach

Cited by 99 publications

References 1 publication

Effectiveness of Bioremediation Processes of Hydrocarbon Pollutants in Weathered Drill Wastes

Effectiveness of Bioremediation Processes of Hydrocarbon Pollutants in Weathered Drill Wastes

Persistence and biodegradation of oil at the ocean floor following Deepwater Horizon

Phytoremediation of Alkylated Polycyclic Aromatic Hydrocarbons in a Crude Oil-Contaminated Soil

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