Use of high-specific RAPD-primers for PCR-analysis of harmful and beneficial insect populations

Kil, V. I.

doi:10.3103/s1068367415010103

Cited by 7 publications

(2 citation statements)

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“…As a result we proposed a new approach to obtaining of reproducible results of the RAPD PCR, which is based on identification and use of high specific primers. Such method allows conducting reproducible RAPD analysis of populations without preliminary evaluation of DNA concentrations and standardization of PCR conditions to this parameter [2].…”

Section: Opinionmentioning

confidence: 99%

Using RAPD PCR Method for Studying DNA Polymorphism of Agricultural Importance Arthropods

Vi¹

2017

ARTOAJ

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Section: Opinionmentioning

confidence: 99%

Using RAPD PCR Method for Studying DNA Polymorphism of Agricultural Importance Arthropods

Vi¹

2017

ARTOAJ

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“…The popularity of random amplified polymorphic DNA (RAPD) stems from its ability to generate numerous polymorphic loci from minimal DNA without prior template knowledge ( Babu et al 2013 ). RAPD markers effectively distinguish geographically and genetically isolated populations within species, elucidating populations arising through drift or selection under varying conditions ( Vaughn and Antolin 1998 , González et al 2007 , Kil 2015 , Mohammadi Sharif et al 2022 ). In Egypt, Aiolopus thalassinus is a predominant grasshopper species with wide Palearctic, Afrotropical, Oriental, and Australian distribution ( Hollis 1968 , GBIF Secretariat 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Limited genetic variability and spatial population structure in grasshoppers between natural and metal-contaminated areas in Egypt

Soliman,

Almadiy,

Al-Akeel

et al. 2024

Journal of Insect Science

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Pollutants in an environment can have long-term implications for the species living there, resulting in local adaptations with implications for their genetic structure. Heavy metal pollutants infiltrate soils and groundwater, bioaccumulate in food webs, and negatively impact biota. In this study, we investigated the degree to which the genetic structure and variability of the slender green-winged grasshopper (Aiolopus thalassinus (Fabricius) (Orthoptera: Acrididae)) were impacted by heavy metal pollution and distance. We used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method to examine the genetic variability of populations in 3 heavy metal-polluted and 3 unpolluted locations across varying geographical distances in Egypt. The heavy metal concentrations of cadmium, copper, lead, and zinc were measured from the grasshopper tissue and soils. Sixty-nine unique and polymorphic bands were produced by 4 primers. Cluster and principal component analyses separated the populations inside and outside Cairo into 2 main branches, which were further divided into smaller branches corresponding to their geographical regions. We found no differences in the Shannon genetic diversity index between populations or with increasing heavy metal concentrations in either the soil or the grasshopper tissue. Our results showed a greater genetic variation among populations than between populations within the same location, indicating populations within locations were less differentiated than those between locations. The moderate correlation between genetic similarity and spatial distance suggests geographical isolation influenced grasshopper population differentiation. Based on the RAPD analysis, environmental pollutants and geographical distances impact the A. thalassinus population structure, potentially restricting gene flow between sites even at small spatial scales.

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Molecular basis of animal systematics including barcoding

Goyal

Sobti

2022

Advances in Animal Experimentation and Modeling

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Use of high-specific RAPD-primers for PCR-analysis of harmful and beneficial insect populations

Cited by 7 publications

References 3 publications

Using RAPD PCR Method for Studying DNA Polymorphism of Agricultural Importance Arthropods

Using RAPD PCR Method for Studying DNA Polymorphism of Agricultural Importance Arthropods

Limited genetic variability and spatial population structure in grasshoppers between natural and metal-contaminated areas in Egypt

Molecular basis of animal systematics including barcoding

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