Use of high-performance liquid chromatography to quantitate the opiate and sugar content of illicit heroin preparations

White, P.C.; Jane, I.; Scott, A.; Connett, B. E.

doi:10.1016/s0021-9673(01)96724-x

Cited by 20 publications

(5 citation statements)

References 10 publications

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“…One of the major caveats associated with HPLC is that less routinely used chemicals like hexylamine and octane sulfonic acid sodium may be required to quantitate morphine and other opiates (Lurie & Carr 1986; Lee et al 2005). Besides, peak fronting and peak tailing of morphine were evident in some studies (White et al 1983; Baker & Gough 1981). A better peak shape is only obtained, usually accompanied by a tedious preparation of mobile phase (Twitchett 1975).…”

Section: Introductionmentioning

confidence: 89%

Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

Chan

2017

Egypt J Forensic Sci

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BackgroundMorphine is illegal to use unless it is prescribed by a medical doctor. This compound is commonly abused in the form of illicit heroin.MethodsAn easy-to-use method for determining morphine in illicit heroin using high performance liquid chromatography-photodiode array detector (HPLC-PDA) was developed. With the aid of 50:50 acetonitrile:ammonium formate, this target compound traveling in a pentafluorophenyl (PFP) column was successfully separated from other compounds frequently present in the heroin matrix.ResultsThe method was precise with an intra-day relative standard deviation (RSD) ≤ 0.8% and inter-day RSD < 5%. It was able to detect as low as 0.001 mg/mL morphine. The detector’s response was linear (R 2 > 0.999) and reliable for morphine quantitation from 0.005 up to 1 mg/mL. Through recovery studies, accuracy of the method was averagely estimated to be 89.54–101.91%.ConclusionsThis method saves time in terms of mobile phase preparation and cuts cost by excluding additional purchase of expensive chemicals. More importantly, it proves to be able to determine the target analyte with sufficient accuracy and precision.

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Section: Introductionmentioning

confidence: 89%

Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

Chan

2017

Egypt J Forensic Sci

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“…Analytical studies in illicit heroin for the identification of 03-monoacetylmorphine has been performed using a thin-layer chromatographic (TLC) and HPLC method (54), as well as capillary GC and HPLC procedures (55). The characterization and comparison of illicit heroin have been performed by HPLC (56), capillary GC (57,58), and a combination of GC and HPLC (59)(60)(61)(62). A dual column HPLC method has been used to quantify the opiate and sugar content of illicit heroin preparations (62).…”

Section: Drugs and Poisonsmentioning

confidence: 99%

“…The characterization and comparison of illicit heroin have been performed by HPLC (56), capillary GC (57,58), and a combination of GC and HPLC (59)(60)(61)(62). A dual column HPLC method has been used to quantify the opiate and sugar content of illicit heroin preparations (62). Manufacturing impurities in illicit heroin after derivatization with heptafluorobutyric anhydride have been determined at picogram levels by using fused silica capillary column GC in the splitless mode with an electron capture detector (63,64).…”

Section: Drugs and Poisonsmentioning

confidence: 99%

Forensic science

Brettell¹,

Saferstein²

1985

Anal. Chem.

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“…Microscopic techniques such as optical crystallography are highly dependent on operator skill (2), and become difficult to interpret with low levels of carbohydrates. Gas chromatography (GC) (3–6) and high‐performance liquid chromatography (HPLC) (2,7,8) have been used for the analysis of sugars and polyhydric alcohols in seized heroin exhibits. GC, unlike HPLC, requires derivatization and allows for the separation of anomers of reducing sugars, which can increase the complexity of analysis.…”

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confidence: 99%

Analysis of Carbohydrates in Seized Heroin Using Capillary Electrophoresis

Lurie

Hays

Valentino

2006

Journal of Forensic Sciences

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Illicitly produced heroin is commonly cut with carbohydrates to increase bulk. The analysis of these solutes is important for legal and intelligence purposes. A capillary electrophoresis (CE) method was developed for the qualitative analysis of dextrose, lactose, sucrose, inositol, and mannitol in heroin exhibits. For this method, a 64 cm (55.5 cm to detector window) by 50 mum capillary was used with the Agilent Basic Anion Buffer modified to pH 12.1. This separation was performed at 25 degrees C with a voltage of 20 kV and indirect detection with 2,6-pyridinedicarboxylic acid as the visualization reagent. The methodology is also applicable for the screening of inorganic and organic anions using indirect detection, and acidic adulterants using direct detection. For a run time of 13 min, the relative standard deviation (n = 6) of the methodology was better than 0.36% for migration times and less than 2.6% for corrected peak areas. For the analysis of carbohydrates and acidic adulterants in seized heroin, excellent agreement was obtained between CE and nuclear magnetic resonance spectroscopy.

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Use of high-performance liquid chromatography to quantitate the opiate and sugar content of illicit heroin preparations

Cited by 20 publications

References 10 publications

Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

Forensic science

Analysis of Carbohydrates in Seized Heroin Using Capillary Electrophoresis

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