Use of HepG2 cell line for direct or indirect mutagens screening: comparative investigation between comet and micronucleus assays

A cell-microelectronic sensing technique for profiling cytotoxicity of chemicals

“…One study looked at the inhibition of RNA synthesis in HepG2 cells by NDMA, without the addition of an S9 fraction. This study found an IC 50 of 110 ± 24 mM [22], which is similar to our IC 50 value of 95 mM (95% confidence interval = 77-118 mM) (Fig. 2).…”

“…This can be accomplished by taking advantage of the parallel screening abilities of the RT-CES system to monitor multiple concentrations and cell lines at the same. To demonstrate the proof of principle, four cell lines, namely CHO-K1, A549, T24, and HepG2, were used in this study because they have been used previously in cytotoxicity testing assays [4,[11][12][13][22][23][24][25] and all are easily available. In addition, CHO-K1 (Chinese hamster ovary) cells are non-cancerous and have been previously utilized in a number of cytotoxicity studies of other DBPs [11][12][13], which may be useful for comparisons between datasets in the future.…”

The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach

“…Electrophoresis can be carried out under highly alkaline conditions (pH > 12.6) in order to detect single and double-strand breaks and alkali-labile lesions. The assay was performed following the protocol of Singh, McCoy, Tice, and Schneider (1988) with some modifications for cell preparation (Valentin-Séverin, Le Hégarat, Lebon, Lhuguenot, & Chagnon, 2003). Shortly, after the treatment, cell suspensions were prepared by washing the cells with PBS and treating them with trypsin/EDTA for 5 min at 37°C.…”

Ensaio cometa em microalgas marinhas: danos no DNA de Dunaliella tertiolecta Butcher 1959 causados pela exposição à 4-nitroquinolina-N-óxido e ao benzo [a] pireno

“…22) com resultados de cometa em outros organismos ou células, podemos observar que D. tertiolecta possui alta sensibilidade. Ela apresentou maior sensibilidade que crustáceos (embriões de camarão, 2 µM por 24 h) (Kim & Lee, 2004), que fibroblastos humanos (1 µM por 1 h) (Hömme et al, 2000), que células de hepatoma humano (1 µM por 4 h) (Valentin-Severin et al, 2003) e que ratos (0,26 mM por 4 semanas, administrado oralmente) (Ribeiro et al, 2004). Por outro lado, dados de manual do Tritek CometScore).…”