Use of Hemicellulose Hydrolysate for β-Glucosidase Fermentation

Réczey, Kati; Brumbauer, Anikó; Bollok, Monika; Szengyel, Zsolt; Zacchi, Guido

doi:10.1007/978-1-4612-1814-2_22

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…The presence of ␤-glucosidases in close proximity to the fungal cell wall may limit loss of glucose to the environment following cellulose hydrolysis. T. reesei produces ␤-glucosidases at low levels compared to other fungi such as Aspergillus species (560). Furthermore, the ␤-glucosidases of T. reesei are subject to product (glucose) inhibition (102,217,417) whereas those of Aspergillus species are more glucose tolerant (138,231,724,768).…”

Section: Cellulase Enzyme Systemsmentioning

confidence: 99%

“…The levels of T. reesei ␤-glucosidase are presumably sufficient for growth on cellulose, but not sufficient for extensive in vitro saccharification of cellulose. T. reesei cellulase preparations, supplemented with Aspergillus ␤-glucosidase, are considered most often for cellulose saccharification on an industrial scale (560,644).…”

Section: Cellulase Enzyme Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,004

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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Section: Cellulase Enzyme Systemsmentioning

confidence: 99%

Section: Cellulase Enzyme Systemsmentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,004

2,868

View full text Add to dashboard Cite

show abstract

“…Extensive work has been done on developing inhibitor tolerant and pentose utilizing yeast strains [5,9]. The other option is to use this fraction for on-site cellulase production by Trichoderma reesei [8,20,23]. However, these efforts with Trichoderma have mostly failed when the total concentration of the inhibitors exceeded the level of tolerance of T. reesei.…”

Section: Enhanced Utilization Of Sweet Sorghum In Bioethanol Productimentioning

confidence: 99%

Processing sweet sorghum into bioethanol – an integrated approach

Gyalai-Korpos¹,

Fülöp²,

Sipos³

et al. 2012

Per. Pol. Chem. Eng.

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Numerous evidences have been provided that juice of sweet sorghum and the leftover after squeezing, the bagasse can be a proper feedstock for bioethanol production. The possibility to integrate a side stream of sweet sorghum processing into the biomass-to-ethanol process was investigated in this study. The liquid fraction, a side stream of the necessary pretreatment of the bagasse was utilized as carbon source for Trichoderma reesei RUT-C30 to produce cellulase enzymes for biomass conversion. However, to overcome the inhibitory nature of the liquid fraction, pre-adaptation of the fungus on solid media was carried out previous to submerged fermentations. The results show that with this approach the lag phase caused by the inhibitors could be markedly shortened and an enhancement of the final enzyme production could be achieved when comparing the preadapted strains to reference.

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Use of Hemicellulose Hydrolysate for β-Glucosidase Fermentation

Cited by 2 publications

References 21 publications

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Processing sweet sorghum into bioethanol – an integrated approach

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