Use of headspace solid-phase microextraction (HS-SPME) in hair analysis for organic compounds

Sporkert, Frank; Pragst, F.

doi:10.1016/s0379-0738(99)00158-9

Cited by 120 publications

(77 citation statements)

References 31 publications

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“…The amount of analyte absorbed/adsorbed by the fiber is proportional to its initial concentration in the sample [20]. HS-SPME is ideal for the analyses of volatile and even semi-volatile compounds in biological specimens due the fact that interference from fixed components present in the matrix is reduced, yielding cleaner extracts [21,22].…”

Section: Introductionmentioning

confidence: 99%

Headspace solid‐phase microextraction of cannabinoids in human head hair samples

Oliveira¹,

Yonamine²,

Moreau³

2007

J of Separation Science

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A fast method was optimized and validated with the aim to detect cannabinoids (cannabidiol, cannabinol, and delta-9-tetrahydrocannabinol) in human head hair samples. The method was based on an initial procedure of external decontamination of hair samples (10 mg) with petroleum ether, followed by alkaline digestion and further extraction of cannabinoids by means of a headspace solid-phase microextraction technique (HS-SPME). GC-MS was used to identify and quantify the analytes in SIM mode. The LOQs and LODs obtained were 0.07 and 0.12 ng/mg, respectively, for all the studied cannabinoids. The method proved to be simple, rapid, and precise. By using the weighted least squares linear regression (weighting factor 1/x2), the accuracy of the analytical method was improved at the lower end of the calibration curve (from 0.12 to 12 ng/mg; r >0.98). Hair samples collected from eight volunteers (in-patients of a drug abuse rehabilitation clinic) were submitted to the proposed method. Detection of the drugs was observed in samples of the volunteers who reported frequent marijuana use (at least ten times a week).

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Section: Introductionmentioning

confidence: 99%

Headspace solid‐phase microextraction of cannabinoids in human head hair samples

Oliveira¹,

Yonamine²,

Moreau³

2007

J of Separation Science

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“…In fact, HS-SPME, an alternative application of SPME, has been used in different methods to detect drugs in several kinds of samples [14]. HS-SPME is ideal for the analyses of volatile and even semi-volatile compounds in biological specimens because interferences from fixed components present in the matrix are reduced, yielding cleaner extracts for analysis [14,15]. Besides feasibility, the other main advantage of HS-SPME procedure is that toxic solvents are not necessary for extraction.…”

Section: Hs-spme Proceduresmentioning

confidence: 99%

Determination of eight fatty acid ethyl esters in meconium samples by headspace solid‐phase microextraction and gas chromatography–mass spectrometry

Roehsig

Paula

Moura

et al. 2010

J of Separation Science

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A number of fatty acid ethyl esters (FAEEs) have recently been detected in meconium samples. Several of these FAEEs have been evaluated as possible biomarkers for in utero ethanol exposure. In the present study, a method was optimized and validated for the simultaneous determination of eight FAEEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate and ethyl arachidonate) in meconium samples. FAEEs were extracted by headspace solid-phase microextraction. Analyte detection and quantification were carried out using GC-MS operated in chemical ionization mode. The corresponding D5-ethyl esters were synthesized and used as internal standards. The LOQ and LOD for each analyte were <150 and <100 ng/g, respectively. The method showed good linearity (r(2)>0.98) in the concentration range studied (LOQ-2000 ng/g). The intra- and interday imprecision, given by the RSD of the method, was lower than 15% for all FAEEs studied. The validated method was applied to 63 authentic specimens. FAEEs could be detected in alcohol-exposed newborns (>600 ng/g cumulative concentration). Interestingly, FAEEs could also be detected in some non-exposed newborns, although the concentrations were much lower than those measured in exposed cases.

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“…Instead of LLE, the employment of SPE is also possible. Furthermore, the solid phase microextraction (SPME) in combination with head-space analysis is usable [104][105][106]. In the case of using hair samples, possible external contamination (e.g., by passive smoking of Cannabis) has to be considered as false positive result.…”

Section: Sample Preparationmentioning

confidence: 99%

Chemistry and Biological Activity of Tetrahydrocannabinol and its Derivatives

Flemming

Muntendam

Steup³

et al. 2007

Topics in Heterocyclic Chemistry

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Use of headspace solid-phase microextraction (HS-SPME) in hair analysis for organic compounds

Cited by 120 publications

References 31 publications

Headspace solid‐phase microextraction of cannabinoids in human head hair samples

Headspace solid‐phase microextraction of cannabinoids in human head hair samples

Determination of eight fatty acid ethyl esters in meconium samples by headspace solid‐phase microextraction and gas chromatography–mass spectrometry

Chemistry and Biological Activity of Tetrahydrocannabinol and its Derivatives

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