Use of hand held photopolymerizer to photoinactivate Streptococcus mutans

Paulino, Tony de Paiva; Ribeiro, Karina F.; Thedei, Geraldo; Tedesco, Antônio Cláudio; Ciancaglini, Pietro

doi:10.1016/j.archoralbio.2004.09.002

Cited by 94 publications

(70 citation statements)

References 42 publications

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“…1A). This is in agreement with previously published reports using blue light (400-500 nm) to photoactivate RB [10,12]. Also, light irradiation alone (240 s) had no effect on E. faecalis but reduced F. nucleatum viability by approximately 10%.…”

Section: Discussionsupporting

confidence: 93%

“…The use of blue light-mediated aPDT has stemmed from the widespread availability of dental blue-light sources having a broad emission spectrum (400-500 nm) and a high energy per photon [11]. Blue light-activated rose bengal was shown to inactivate S. mutans, A. actinomycetemcomitans and C. albicans in several research reports that also confirmed the influence of the amount of light energy delivered on bacterial killing [10,12,13].…”

Section: Introductionmentioning

confidence: 96%

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Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Manoil

Filieri

Schrenzel

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry Antibacterial photodynamic therapy (aPDT) using rose bengal (RB) and blue-light kills bacteria through the production of reactive oxygen derivates. However, the interaction mechanism of RB with bacterial cells remains unclear. This study investigated the uptake efficiency and the antibacterial activity of blue light-activated RB against Enterococcus faecalis and Fusobacterium nucleatum. Spectrophotometry and epifluorescence microscopy were used to evaluate binding of RB to bacteria. The antibacterial activity of RB after various irradiation times was assessed by flow cytometry in combination with cell sorting. Uptake of RB increased in a concentration dependent manner in both strains although E. faecalis displayed higher uptake values. RB appeared to bind specific sites located at the cellular poles of E. faecalis and at regular intervals along F. nucleatum. Blue-light irradiation of samples incubated with RB significantly reduced bacterial viability. After incubation with 10 μM RB and 240 s irradiation, only 0.01% (± 0.01%) of E. faecalis cells and 0.03% (±0.03%) of F. nucleatum survived after treatment. This study indicated that RB can bind to E. faecalis and F. nucleatum in a sufficient amount to elicit effective aPDT. Epifluorescence microscopy showed a yet-unreported property of RB binding to bacterial membranes. Flow cytometry allowed the detection of bacteria with damaged membranes that were unable to form colonies on agars after cell sorting.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 96%

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Manoil

Filieri

Schrenzel

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…Paulino et al used a dental blue light source to activate a xanthene derivate called rose bengal, and have reported a complete killing of Streptococcus mutans grown in planktonic suspensions [5]. The antibacterial properties of Photosan light-activated with a commercially available dental photopolymerizer emitting blue light were reported against the Gram-positive S. mutans and Enterococcus faecalis in planktonic cultures [6].…”

Section: Introductionmentioning

confidence: 99%

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

Marinic

Manoil

Filieri

et al. 2015

Photodiagnosis and Photodynamic Therapy

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Background: In dentistry, antibacterial photodynamic therapy (a-PDT) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. In the current study, we assessed the ability of eosin Y exposed to 3 irradiation protocols at inactivating Enterococcus faecalis biofilms, in vitro. Methods: E. faecalis biofilms formed on hydroxyapatite disks were incubated with eosin Y (10-80 M), then activated with blue light using different irradiation protocols. Biofilms exposed to continuous exposure were incubated for 40 min before being light-activated for 960 s. For the intermittent exposure, biofilms were exposed 4 times to the light/photosensitizer combination (960 s total) without renewing the photosensitizer. For repeated a-PDT, the same light dose was delivered in a series of 4 irradiation periods separated by dark periods; fresh photosensitizer was added between each light irradiation. After treatment, bacteria were immediately labeled with LIVE/DEAD BacLight Bacterial Viability kit and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (˛= 0.05). * Corresponding author. Results:The viability of E. faecalis biofilms exposed to 10 M eosin Y, was significantly reduced compared to controls (light only-eosin Y only). After a second exposure to blue light-activated eosin Y, viability significantly decreased from 58% to 12% whereas 6.5% of the bacterial biofilm remained live after a third exposure (p < 0.05). Only 3.5% of the bacterial population survived after the fourth exposure. Conclusions:The results of this study indicate that blue light-activated eosin Y can photoinactivate E. faecalis biofilms grown on hydroxyapatite disks. Also, repeated exposures to blue light-activated eosin Y were shown to significantly improve efficacy. Further studies seem warranted to optimize the antibacterial activity of blue light-activated eosin Y on major oral pathogens.

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“…12,13 It has been demonstrated that PDT is effective against oral species and may not promote damage to host cells and tissues. 14,15 However, most studies on cell damage are short-term investigations and safety studies have been performed over the short term. Kömerik et al 15 observed complete inactivation of Porphyromonas gingivalis in the maxillary molar region of rats after PDT, with no adverse effects on the periodontal structures after 3 days and significant reductions in bone loss after 90 days.…”

mentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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129

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Use of hand held photopolymerizer to photoinactivate Streptococcus mutans

Cited by 94 publications

References 42 publications

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

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