Use of growth retardants for banana (Musa AAA cv. Grand Naine) shoot multiplication in temporary immersion systems

Albany, N.; González, Elio Jiménez; Vilchez, J.; García, L. R.; Feria, Manuel de; Pérez, Naivy; Sarría, Zoe; Pérez, Blanca; Clavelo, Justo

doi:10.1007/1-4020-3200-5_13

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…(WILKEN et al, 2014;DO NASCIMENTO VIEIRA et al, 2015), pero puede decirse, como norma general en horticultura, que un incremento en la proporción de luz azul tiene un efecto sobre el acortamiento del tamaño de las plantas y consecuentemente provoca un crecimiento más compacto (WOLLAEGER, 2014). Esto hace que el uso de la luz azul resulte beneficioso desde el punto de vista de la propagación in vitro, especialmente en la multiplicación de bananos y plátanos en sistemas de cultivo en medio líquido, incluyendo el uso de biorreactores de inmersión temporal, en los que un excesivo crecimiento de los brotes puede suponer una limitación (Albany et al, 2005). Por otro lado, también parece probable que pueda existir un máximo en la proporción de luz azul para cada especie, a partir del cual pueda inhibir tanto el crecimiento in vitro, como el posterior desarrollo de las plantas (MITCHELL et al, 2015).…”

Section: Medios De Cultivounclassified

Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones

Galan¹,

Rangel²,

López

et al. 2018

Rev. Bras. Frutic.

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Resumen Se pasa revista a los diferentes tipos de propagación del banano tradicional (fundamentalmente hijos y rizomas o partes del mismo), aún utilizada en plantaciones de tipo familiar y en plantaciones establecidas en gran número de países tropicales dedicadas al consumo local, y a través de cultivo in vitro (cultivo de tejidos), utilizada en las modernas explotaciones de bananos dedicados a la exportación. El trabajo se inicia con una descripción de las características morfológicas y desarrollo de la planta, una breve descripción de las estructuras de propagación a la que siguen luego dos grandes apartados: Propagación tradicional y microprogación. En este último apartado se aborda la propagación por organogénesis, mediante la micropropagación tradicional en medios de cultivos semisólidos y más actual por bioreactores y la propagación por embriogénesis somática. Se discute en profundidad los medios de cultivo e iluminación requeridos durante las diferentes fases de propagación por cultivo in vitro y se finaliza abordando los aspectos de endurecimiento, aclimatación y trasplante al campo de las plantas propagadas por cultivo de tejidos.

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Section: Medios De Cultivounclassified

Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones

Galan¹,

Rangel²,

López

et al. 2018

Rev. Bras. Frutic.

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“…In the case of banana, increases have been achieved in high average multiplication rates, with improved plant quality (Albany et al, 2005;Berthouly and Etienne, 2005;Watt, 2012;Pérez et al, 2013). Only a few bamboo species such as Dendrocalamus latiflorus (Mongkolsook et al, 2005 cited by García-Ramirez et al, 2014), Bambusa ventricosa and Dracaena deremensis (Chaille, 2011) and Bambusa vulgaris (García-Ramírez et al, 2014) have been propagated by TIS.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

GutiÃ©rrez¹,

Lopez-Franco²,

Morales-PinzÃ3n³

2016

Afr. J. Biotechnol.

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The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semisolid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA ® ), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and use of semi-solid culture medium, which consisted of MS supplemented with 3.0 mg L -1 of the cytokinin benzylaminopurine (BAP). Equipment consisted of 20 vessels for automated RITA ® , each containing 200 ml of culture medium. Immersions were performed for 2 min at two different frequency intervals (6 and 8 h). Large clumps of G. angustifolia with 1, 2 or 3 stems were inoculated depending on the treatment. Best results were obtained with four immersion cycles per day (every 6 h), with a multiplication index of 2.7 shoots per original explant (axillary buds) and greater rhizome growth. Overall, the temporary immersion system performed better than the semi-solid medium in terms of shoot multiplication rates and rhizome growth. Further studies should be conducted to develop an application for RITA ® for use in the commercial production of G. angustifolia.

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“…Ancymidol (ANC) and paclobutrazol in liquid-based micropropagation systems reduced water uptake and vacuolization while decreasing internode length and leaf size (Ziv, 2005). Ancymidol and paclobutrazol in liquid also reduced plant size and increased the number of divisions in small, shaken flasks of Musa acuminata Colla 'Grand Naine' (Albany et al, 2005). Ancymidol reduced plant size and increased occurrence of divisions of Hosta 'Blue Vision' over a prolonged period of the culture cycle while in small, shaken jars (Maki et al, 2005).…”

mentioning

confidence: 99%

“…Ancymidol reduced plant size and increased occurrence of divisions of Hosta 'Blue Vision' over a prolonged period of the culture cycle while in small, shaken jars (Maki et al, 2005). Because there is less leaf and root tissue to be cut away and discarded, smaller plants are handled more quickly during aseptic transfer processes, lowering the costly input of hood labor (Adelberg and Toler, 2004;Adelberg et al, 2005;Albany et al, 2005).…”

mentioning

confidence: 99%

In vitro Sugar and Water Use in Diploid and Tetraploid Genotypes of Daylily (Hemerocallis spp.) in Liquid Medium as Affected by Density and Plant Growth Regulators

Adelberg¹,

Delgado²,

Tomkins³

2007

horts

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Two tetraploid and two diploid genotypes of Hemerocallis spp. were micropropagated on an orbital shaker in Murashige and Skoog liquid medium in a factorial combination of two sucrose concentrations (90 mm and 180 mm), two 6-benzylaminopurine (benzyladenine) concentrations (0.32 μm and 3.2 μm), at two densities (57 explants/L and 171 explants/L), in the presence (0.32 μm) and absence of α-cyclopyl-α-[4-mehtoxyphenyl]-5-pyrimididinmethanol (ancymidol). There were linear relationships between fresh weight and water use (R 2 = 0.800, P < 0.0001), dry weight and sucrose use (R 2 = 0.636, P < 0.0001), and relative dry weight (dry weight/fresh weight = relative dry weight) to concentration of sucrose residual in medium after culture (R 2 = 0.553, P < 0.0001). Eighty-five percent of the water used and 74% of the sucrose used were incorporated as plant fresh weight and dry weight, respectively. A 1% increase in percent sucrose residual (mass/volume in spent medium) was correlated to an increase of 1.8% relative dry weight over the range 7% to 22% relative dry weight. In vessels with 90 mm initial sucrose, where the most growth had occurred (>15 g fresh weight), sucrose was depleted (<0.2% sucrose) and plantlets had the lowest relative dry weight (≈6.9%). In vessels from 180 mm initial sucrose, with similarly high fresh weight, plantlets had 12.0% relative dry weight with 2.1% sucrose residual in medium. Fresh weight, dry weight, or relative dry weight of plantlets in the laboratory did not correlate with subsequent survival or growth in the greenhouse. Plantlets grown without ancymidol at the lower benzyladenine concentration acclimatized to the greenhouse with 93% survival. However, greenhouse survival of plants grown with ancymidol and a higher level of benzyladenine was only 4%. ‘Barbara Mitchell’ was the largest plant in the laboratory, but often had poorest growth in the greenhouse. When optimizing a liquid micropropagation protocol for larger vessels, sucrose and water requirements may be directly related to targeted biomass yield, but each genotype needs to be handled independently with ex vitro validation of plant vigor.

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Use of growth retardants for banana (Musa AAA cv. Grand Naine) shoot multiplication in temporary immersion systems

Cited by 6 publications

References 14 publications

Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones

Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

In vitro Sugar and Water Use in Diploid and Tetraploid Genotypes of Daylily (Hemerocallis spp.) in Liquid Medium as Affected by Density and Plant Growth Regulators

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