Use of Grafted Seedlings for Vegetable Production in Japan

Oda, Masayuki

doi:10.17660/actahortic.2008.770.1

Cited by 9 publications

(8 citation statements)

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“…The hybrid cucumber scions of 13 days and pumpkin rootstocks of 10 days were best match for grafting. Rootstock was removed just above the false leaf, and the sharpened scion was inserted in it after making a hole between the false leaf interjections with the help of small narrow sterilized wooden sticks [20,38]. The union point was sealed with aluminum wrap and grafting clips.…”

Section: Grafting Techniques and Proceduresmentioning

confidence: 99%

“…Several studies indicated that the grafting technique showed productive findings such as increase growth, yield and tolerance against stress conditions [35][36][37]. These plant characteristics majorly linked with genotype of rootstock particularly plant growth and yield effected by rootstock [37,38]. The grafting of cucumber crop on different rootstock reduces precocity but the total yield significantly increased as compared with non-grafted plants [39].…”

Section: Introductionmentioning

confidence: 99%

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Interactive Effects of Grafting Techniques and Scion-Rootstocks Combinations on Vegetative Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

et al. 2019

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The density of herbaceous crops creates a suitable environment to produce pathogens in the soil that intensify the attack of pathogens traditionally controlled by disinfectant, which are mostly prohibited and unlisted because of their toxicity. Grafting is an alternative technique to enhance abiotic stress tolerance and reduce root diseases due to soil-borne pathogens, thus enhancing crop production. This research study was conducted during the crop season of 2017 and 2018 in order to investigate the interactive effect of different grafting techniques of hybrid scion onto local rootstocks on plants survival, plant phenological growth, fruit yield and fruit quality under a controlled environment. The hybrid cucumber was also planted self-rooted. The cucumber (Cucumis sativus L.) cv. Kalaam F1, Syngenta was grafted onto four local cucurbitaceous rootstocks; ridge gourd (Luffa operculate Cogn.), bitter gourd (Momordica charantia L.), pumpkin (Cucurbita pepo L.), bottle gourd (Lagenaria siceraria (Molina) Standl.) using splice grafting, tongue approach, single cotyledon and hole insertion grafting techniques and self-rooted hybrid cucumber under greenhouse conditions. The experimental results indicated that all local cucurbitaceous rootstocks showed a high compatibility with hybrid cucumber scion in the splice grafting method compared to other grafting and non-grafted methods. Lagenaria siceraria rootstocks were found highly compatible with cucumber cv Kalaam scion which gave significantly maximum plant survival rates (95%) due to high sap contents, high SPAD value, better vegetative growth and maximum fruit yield when compared with other rootstocks by employing the splice grafting method followed by tongue approach, single cotyledon and hole insertion grafting while the fruit quality of all rootstocks was observed to be similar. The non-grafted cucumber cv. Kalaam F1 showed significant results of plant vegetative growth, fruit development and fruit quality and encountered grafting methods while the lowest result were associated with the hole insertion grafting method in all scion/rootstock combinations. The grafted plants have no significant effect on cucumber fruit dry matter and fruit quality while the fruit mineral compositions (N, P, K, Ca and Mg) were higher among grafted and non-grafted plant fruits. The results indicate that grafting hybrid cucumber onto four local cucurbitaceous rootstocks influenced growth, yield and fruit quality. Grafting can be alternative and control measure for soil-borne disease and to enhance cucumber production.

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Section: Grafting Techniques and Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Grafting Techniques and Scion-Rootstocks Combinations on Vegetative Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

et al. 2019

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“…However, it is difficult to improve the tolerance of cucumber to pathogen attack by conventional breeding owing to its narrow genetic base, with a genetic variability of only 3–8 % (Plader et al 2007). Although grafting of cucumber plants onto cucurbitaceous rootstocks is a common way to avoid soilborne diseases and nematodes (Oda 2008), this technique is labor-intensive. Genetic engineering is a powerful way to improve biotic stress tolerance, because useful genes can be transferred from other species.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Nanasato

Konagaya

Okuzaki

et al. 2012

Plant Biotechnol Rep

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An improved method for genetic transformation of cucumber (Cucumis sativus L. cv. Shinhokusei No. 1) was developed. Vacuum infiltration of cotyledonary explants with Agrobacterium suspension enhanced the efficiency of Agrobacterium infection in the proximal regions of explants. Co-cultivation on filter paper wicks suppressed necrosis of explants, leading to increased regeneration efficiency. Putative transgenic plants were screened by kanamycin resistance and green fluorescent protein (GFP) fluorescence, and integration of the transgene into the cucumber genome was confirmed by genomic polymerase chain reaction (PCR) and Southern blotting. These transgenic plants grew normally and T1 seeds were obtained from 7 lines. Finally, stable integration and transmission of the transgene in T1 generations were confirmed by GFP fluorescence and genomic PCR. The average transgenic efficiency for producing cucumbers with our method was 11.9 ± 3.5 %, which is among the highest values reported until date using kanamycin as a selective agent.Electronic supplementary materialThe online version of this article (doi:10.1007/s11816-012-0260-1) contains supplementary material, which is available to authorized users.

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“…This factor is due to the fixed value of the velocities of the pneumatic devices in response to an incremental increase in the robot velocities ( Figure 11). Numerous studies have collected the test results of prototypes and commercial devices for the automated grafting of horticultural seedlings over the last three decades [3,5,7,23,[36][37][38][39][40][41][42][43][44], and the results for dozens of pieces of equipment have been collected, mainly from Southeast Asia (Japan, Korea, and China mainly) and Europe.…”

Section: Resultsmentioning

confidence: 99%

Conventional Industrial Robotics Applied to the Process of Tomato Grafting Using the Splicing Technique

et al. 2019

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Horticultural grafting is routinely performed manually, demanding a high degree of concentration and requiring operators to withstand extreme humidity and temperature conditions. This article presents the results derived from adapting the splicing technique for tomato grafting, characterized by the coordinated work of two conventional anthropomorphic industrial robots with the support of low-cost passive auxiliary units for the transportation, handling, and conditioning of the seedlings. This work provides a new approach to improve the efficiency of tomato grafting. Six test rates were analyzed, which allowed the system to be evaluated across 900 grafted units, with gradual increases in the speed of robots work, operating from 80 grafts/hour to over 300 grafts/hour. The results obtained show that a higher number of grafts per hour than the number manually performed by skilled workers could be reached easily, with success rates of approximately 90% for working speeds around 210-240 grafts/hour.

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Use of Grafted Seedlings for Vegetable Production in Japan

Cited by 9 publications

References 0 publications

Interactive Effects of Grafting Techniques and Scion-Rootstocks Combinations on Vegetative Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

Interactive Effects of Grafting Techniques and Scion-Rootstocks Combinations on Vegetative Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Conventional Industrial Robotics Applied to the Process of Tomato Grafting Using the Splicing Technique

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