Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

Pinheiro, João Paulo Silva; Melo-Maciel, M. A. P.; Linhares, Francisco Renan Aragão; Lopes, Júlia Trugilio; Almeida‐Monteiro, Priscila Silva de; Torres, Thaís Maia; Salmito‐Vanderley, Carminda Sandra Brito

doi:10.21451/1984-3143-ar782

Cited by 12 publications

(16 citation statements)

References 18 publications

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“…Existem estudos que esclarecem aspectos sobre a reprodução de P. brevis, inclusive sobre a aplicação da técnica de congelação seminal, que demonstraram a boa congelabilidade do sêmen, utilizando diluentes como a glicose [10,13,17] e a água de coco em pó (ACP-104) [4]. Porém, a coleta seminal geralmente ocorre em locais com poucos recursos laboratoriais, sendo necessária a translocação de equipamentos e mais recursos humanos em campo [21].…”

Section: Introductionunclassified

Influence of Cooling Time and Diluents on the Freezability of Prochilodus brevis semen

Nascimento

Leite-Castro

Montenegro

et al. 2017

Acta Scientiae. Vet.

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Background: Prochilodus brevis is a rheophilic fish of economic and ecological importance. However, anthropic action has made its population vulnerable. Thus, the development of reproductive biotechnologies, such as seminal conservation, is necessary to subsidize their fish farming. However, seminal collections are often performed in places with few laboratory resources, demanding studies to determine the maximum time for which sperm can be cooled, as well as its process until frozen. Thus, the present study aimed to evaluate the influence of cooling time and the presence of dilution solutions on cryopreservation of P. brevis semen.Material, Methods & Results: After seminal collection, nine pools were formed and analyzed for seminal pH, concentration, membrane integrity, morphology and spermatic kinetics - motility, curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). After the analysis of the pools in natura (control 1), they were processed as follows: 1)- immediate freezing (control 2); 2)- cooling: undiluted, diluted in coconut water powder (ACP-104) or diluted in 5% glucose, followed by cooling at different times (6, 12, 24 or 48 h); 3)- Post-refrigeration freezing: the pools were diluted in their respective diluents and 10% dimethyl sulfoxide. After 15 days, the samples were thawed and analyzed for the aforementioned parameters. For the cooled and post-thawed semen, a completely randomized design with 2 (diluent × cooling time) and 3 (storage form × cooling time and storage form × diluent) factors, respectively, was utilized. ANOVA and Dunnett tests were applied to compare the means. In case of seminal cooling, there was no difference (P > 0.05) in sperm motility between control 1 and the undiluted and diluted treatments in ACP-104 for up to 24 h. After 48 h, only the VCL of the sample diluted in ACP-104 was similar (P > 0.05) to that of control 1. When comparing forms of storage (undiluted, diluted in ACP-104 or diluted in glucose) and cooling times, the undiluted samples and the samples diluted in ACP-104 were better (P < 0.05) for all the kinetics parameters analyzed, than those diluted in glucose after 24 h. After 48 h, the cooled semen diluted in ACP-104 presented greater (P < 0.05) motility than the other treated semen samples. The samples diluted in glucose for 48 h presented lower spermatic velocity (P < 0.05) than those subjected to other treatments. Regardless of the diluent used, the post-thawed semen and the cooled semen diluted for 6 h, presented higher sperm kinetic values (P < 0.05) than those of control 2 and other treated samples. Overall, the samples diluted in ACP-104 showed satisfactory results when cooled for up to 48 h or cooled for up to 6 h and frozen.Discussion: This is the first study that froze semen from P. brevis after cooling. Although glucose is a commonly used diluent during seminal freezing and has good post-thawing stability for this species, it is not recommended for cooling before seminal freezing, as prolonged exposure of spermatozoa to glucose may cause osmotic stress to sperm cells. Conversely, good results with ACP-104 might be because of its rich composition, mainly the presence of indole-3-acetic acid (IAA), an auxin with proven potential for seminal conservation of other species. Therefore, for fertilization trials, it is recommended to use ACP-104 as diluent for seminal cooling of P. brevis for up to 48 h or semen that has been frozen after cooling in ACP-104 for a maximum of 6 h.

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Section: Introductionunclassified

Influence of Cooling Time and Diluents on the Freezability of Prochilodus brevis semen

Nascimento

Leite-Castro

Montenegro

et al. 2017

Acta Scientiae. Vet.

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“…In this study, the addition of honey in the diluent showed no significant difference. The acceptable of spermatozoa morphological abnormalities was 20-30% (27). However, the honey addition was better when compared with the control.…”

Section: Discussionmentioning

confidence: 87%

Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

Fanni¹,

Santanumurti²,

Suprayogi³

et al. 2019

IJVS

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Sutchi Catfish is one of the important fish commodities in Indonesia. Unfortunately, its seasonal spawning pattern causes limited supply. Cryopreservation is a solution to solve limited supply since it can store the spermatozoa in low temperature so that physiological, biological and morphological functions still remain. Improving the quality of cryopreservation is important to increase the success of Sutchi Catfish aquaculture. Adding honey in cryopreservation process is expected to increase the quality of spermatozoa since it contains with sugars as a source of spermatozoa's energy. This study tried to compare the effectivity of honey in cryopreservation process with no addition. The treatments used in this study were T1 (0% honey), T2 (0.2% of honey), T3 (0.4% of honey), T4 (0.6% of honey) and T5 (0.8% of honey). 30 days after stored, the spermatozoa were checked their motility, viability, abnormality, fertility and hatching rate. This study showed that honey addition could increase the motility significantly (P<0.01) to 23.14% better than control. The viability increased significantly (P<0.01) to 23.17% better than control. The abnormality test did not show significant difference between honey addition and control although the abnormality value in control was the highest (10.75%). The fertilization rate increased significantly (P<0.01) to 28.85% better than control. The hatching rate increased significantly (P<0.01) to 29.78% better than control. The success of all test indicated that the addition of honey in cryopreservation process of spermatozoa could be performed on Sutchi Catfish to increase its production even though the limited spawning pattern.

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“…Efficient cryopreservation of fish semen using MG was demonstrated in Piracanjuba (Maria, Viveiros, Freitas, & Oliveira, ). Derived from methanol (CH 3 OH) and ethylene oxide (CH 2 OCH 2 ; Viveiros, Oliveira, Maria, Orfão, & Souza, ), MG is considered a non‐toxic alcohol (Pinheiro et al, ; Takagi, Otoi, & Suzuki, ). This explains its popularity as a favourite cryoprotectant in comparison to other alcohols like DMSO (Viveiros et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…MG in concert with BTS also showed high‐quality cryopreservation of semen in Brycon nattereri (Viveiros et al, ). Pinheiro et al (), in a study using four different freezing media, observed that the MG‐containing medium with glucose shows higher motility (70%–76%) as compared to MG and BTS‐containing medium.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of sperm in annual fish Austrolebias minuano (Cyprinodontiformes; Rivulidae)

et al. 2019

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The effects of the cryoprotectants dimethyl sulfoxide, glycerol and methyl glycol at different concentrations on Austrolebias minuano sperm quality parameters were evaluated in this study. The cellular kinetic parameters, determined using flow cytometry, indicated the best results with the samples cryopreserved with 7.5% methyl glycol. Dimethyl sulfoxide concentrations of 7.5% and glycerol concentrations of 10%, 12.5% and 15% demonstrated the least benefit across all evaluated sperm kinetic parameters. When assessed using flow cytometry, a concentration of 7.5% methyl glycol similarly showed better sperm kinetic parameters than 12.5% dimethyl sulfoxide. We conclude that cryoprotectants, especially methyl glycol, are effective for the preservation of sperm quality in A. minuano. However, high sensitivity of spermatozoa against glycerol was observed in these studies; thus, it is not recommended for cryopreservation purposes. K E Y W O R D Scryoprotectants, extinction, killifish, preservation

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Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

Cited by 12 publications

References 18 publications

Influence of Cooling Time and Diluents on the Freezability of Prochilodus brevis semen

Influence of Cooling Time and Diluents on the Freezability of Prochilodus brevis semen

Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

Cryopreservation of sperm in annual fish Austrolebias minuano (Cyprinodontiformes; Rivulidae)

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