Use of Gamithromycin as a Chiral Selector in Capillary Electrophoresis

Ren, Siru; Zhang, Qi; Xue, Song; Liu, Siyao; Rui, Mengjie

doi:10.1016/j.chroma.2020.461099

Cited by 20 publications

(15 citation statements)

References 34 publications

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“…Gamithromycin is a second-generation macrolide used in veterinary medicine composed of a 15-ring structure with two sugar moieties. It has five hydroxyl groups, two tertiary amino groups, a methoxy group, and a propyl group associated with the N atom at the 7α position [ 65 ].…”

Section: Antibiotics As Css In Cementioning

confidence: 99%

“…The separation mechanism was also discussed, and it was concluded that enantio-recognition is based on the formation of hydrogen bonds between the CS hydroxyl group and the primary amine of the enantiomers. It is noticeable that gamithromycin exhibited remarkable enantio-selectivity towards amlodipine (resolution over 15) [ 65 ].…”

Section: Antibiotics As Css In Cementioning

confidence: 99%

See 1 more Smart Citation

The Use of Antibiotics as Chiral Selectors in Capillary Electrophoresis: A Review

Hancu¹,

Papp²,

Szekely-Szentmiklosi³

et al. 2022

Molecules

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Chirality is becoming an essential issue in modern pharmaceutical research as regulatory agencies emphasize the safety and efficiency of enantiomers in drug development. The development of efficient and reliable chiral separation methods became a necessity in the last 30 years, and capillary electrophoresis (CE), due to its relatively low costs and “green” features, is attracting increased attention. Cyclodextrin (CD) and their derivatives are the most frequently used chiral selectors (CSs) in CE, however, the use of antibiotics as CSs represents an interesting alternative. Various classes of antibiotics (aminoglycosides, ansamycins, glycopeptides, lincosamides, macrolides, tetracyclines) have been used more or less successfully for the enantio-separation of pharmaceuticals. Antibiotics offer the possibility of a multitude of potential interactions (electrostatic, inclusion, hydrogen bonding, etc.) due to their chemical diversity, allowing the enantio-separation of analytes with a wide range of structural characteristics. This article aims to review the application of various classes of antibiotics in the CE enantio-separation of pharmaceuticals. Antibiotic physiochemical characteristics, variables impacting enantio-separation, advantages, and disadvantages when certain antibiotics are used as CSs in CE are also explored.

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Section: Antibiotics As Css In Cementioning

confidence: 99%

Section: Antibiotics As Css In Cementioning

confidence: 99%

The Use of Antibiotics as Chiral Selectors in Capillary Electrophoresis: A Review

Hancu¹,

Papp²,

Szekely-Szentmiklosi³

et al. 2022

Molecules

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“…Non-CD synthetic receptors have been employed as BGE additives in CE as well. Gamithromycin (Gam) was reported by Ren et al to show good resolution of the chiral primary amines of primaquine (PMQ) and 1-aminoindan (AMI) [32], with N-methylformamide (NMF) as the non-aqueous solvent [33]. Our lab employed a group of non-CD receptors, tetrasulfonatocalix [4]arene (CX4), hexasulfonatocalix [6]arene (CX6), and cucurbit [7]uril (CB7), for analysis of methylation in peptides (Fig.…”

Section: Synthetic Receptors As Buffer Additivesmentioning

confidence: 99%

Recent (2018–2020) development in capillary electrophoresis

Gao

Zhong

2021

Anal Bioanal Chem

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Development of new capillary electrophoresis (CE) methodology and instrumentation, as well as application of CE to solve new problems, remains an active research area because of the attractive features of CE compared to other separation techniques. In this review, we focus on the representative works about sample preconcentration, separation media or capillary coating development, detector construction, and multidimensional separation in CE, which are judiciously selected from the papers published in 2018-2020.

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“…Recently, Ren et al [ 77 ] reported using the second-generation macrolide antibiotic gamithromycin as a novel chiral selector for enantioseparation in CE. Gamithromycin showed particular enantioseparation power for chiral primary amines.…”

Section: Chiral Capillary Electrokinetic Chromatography Principle and Selectorsmentioning

confidence: 99%

Chiral Capillary Electrokinetic Chromatography: Principle and Applications, Detection and Identification, Design of Experiment, and Exploration of Chiral Recognition Using Molecular Modeling

et al. 2021

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This work reviews the literature of chiral capillary electrokinetic chromatography from January 2016 to March 2021. This is done to explore the state-of-the-art approach and recent developments carried out in this field. The separation principle of the technique is described and supported with simple graphical illustrations, showing migration under normal and reversed polarity modes of the separation voltage. The most relevant applications of the technique for enantioseparation of drugs and other enantiomeric molecules in different fields using chiral selectors in single, dual, or multiple systems are highlighted. Measures to improve the detection sensitivity of chiral capillary electrokinetic chromatography with UV detector are discussed, and the alternative aspects are explored, besides special emphases to hyphenation compatibility to mass spectrometry. Partial filling and counter migration techniques are described. Indirect identification of the separated enantiomers and the determination of enantiomeric migration order are mentioned. The application of Quality by Design principles to facilitate method development, optimization, and validation is presented. The elucidation and explanation of chiral recognition in molecular bases are discussed with special focus on the role of molecular modeling.

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Use of Gamithromycin as a Chiral Selector in Capillary Electrophoresis

Cited by 20 publications

References 34 publications

The Use of Antibiotics as Chiral Selectors in Capillary Electrophoresis: A Review

The Use of Antibiotics as Chiral Selectors in Capillary Electrophoresis: A Review

Recent (2018–2020) development in capillary electrophoresis

Chiral Capillary Electrokinetic Chromatography: Principle and Applications, Detection and Identification, Design of Experiment, and Exploration of Chiral Recognition Using Molecular Modeling

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