Use of Fourier transform infrared (FT-IR) spectroscopy as a tool for pollen identification

Gottardini, E.; Rossi, Stefano; Cristofolini, F.; Benedetti, L.

doi:10.1007/s10453-007-9065-z

Cited by 60 publications

(60 citation statements)

References 23 publications

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“…Among notable additional efforts include the use of X-ray fluorescence (Pepponi et al, 2004) and total internal reflection fluorescence microscopy (TIRFM) (Axelrod, 2008) for the investigation of biological aerosol properties. Infrared vibrational spectroscopy has also been widely used for pollen identification (Pappas et al, 2003;Gottardini et al, 2007;Dell'Anna et al, 2009;Zimmermann, 2010). Scanning electron microscopy (SEM) has been particularly useful at the investigation of PBAP, allowing a close look at the morphology and surface of particles (Karlsson and Malmberg, 1989;Wittmaack et al, 2005;Coz et al, 2010;Po¨schl et al, 2010;Gilardoni et al, 2011).…”

Section: Light Detection and Ranging (Lidar) And Remote Sensingmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,108

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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Section: Light Detection and Ranging (Lidar) And Remote Sensingmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,108

1,114

View full text Add to dashboard Cite

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“…In this work, the authors confirmed that the hierarchical cluster analysis provided valuable information about the reproducibility of FTIR spectra of the same taxon. The utility of infrared spectroscopy in palynology was demonstrated by Gottardini et al (2007). They showed that it is possible to effectively apply this method for the determination of pollen grains to the species level.…”

Section: Introductionmentioning

confidence: 99%

Identification of birch pollen species using FTIR spectroscopy

et al. 2018

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In this study, the morphology and chemical composition of pollen grains of six birch species (Betula utilis Doorenbos, B. dahurica, B. maximowicziana, B. pendula, B. pubescens and B. humilis) were examined to verify which of these features allow distinguishing them in a more unambiguous way. For this purpose, scanning electron microscopy and light microscopy, as well as Fourier transform infrared (FTIR) spectroscopy and curve-fitting analysis of amide I profile, were performed. The microscopy images show that the pollen grains of B. pubescens, B. pendula and B. humilis are similar in diameter and significantly smaller than those of others species, with the largest diameter observed for B. utilis Doorenbos. However, the results obtained from FTIR spectroscopy indicate that the chemical compositions of B. pubescens and B. pendula are similar, but B. humilis is outlaying. Summarizing, it is not possible to unambiguously state, which feature or which technique is the best for differentiating between the six chosen birch species. However, the study showed that both techniques have potential for identification of birch pollen species.

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“…Therefore, the FTIR technique is often used to identify the pollen species (Suphioglu et al 1992). Indeed, there are studies, in which the authors apply spectroscopic methods for the identification of pollen and thus create spectroscopic libraries of plants and pollen (Zimmermann and Kohler 2014; Gottardini et al 2009; Guedes et al 2014). …”

Section: Introductionmentioning

confidence: 99%

FTIR analysis of molecular composition changes in hazel pollen from unpolluted and urbanized areas

et al. 2016

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In this study, the effect of urbanization and environmental pollution on qualitative (structural) and quantitative changes of the Corylus avellana (hazel) pollen was investigated using scanning electron microscopy, Fourier Transform Infrared (FTIR) spectroscopy and curve-fitting analysis of amide I profile. The obtained spectroscopic results show significant variations in the fraction of proteins in the hazel pollen, which probably depend on various degrees of anthropopression. Our results suggest that alterations in the chemical composition of pollen, induced by urbanization and air pollutants, may intensify the allergenic potential and may cause the increase in the incidence of allergies in people. Mutations in nucleic acids are accompanied by a number of molecular changes leading to the formation of allergenic proteins. It seems that the type of habitat, where the pollen grew, affects the individual differentiation. Indeed, it was found that in the site exhibiting low pollution, the hazel pollen contain a lower amount of proteins than to the ones from a site with high anthropopression. Hence, FTIR spectroscopy and curve-fitting analysis of amide I profile can be successfully applied as tools for identifying quantitative and qualitative changes of proteins in hazel pollen.Graphical Abstract Anthropogenic factors such as air pollution and urbanization lead to changes in structure and chemical composition of hazel pollen. Fourier Transform Infrared spectroscopy (FTIR) and Gaussian analysis showed structural changes in hazel pollen collected from sites with different absorbance values of individual chemical functional groups and changes in the secondary structure of proteins of the pollen.

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Use of Fourier transform infrared (FT-IR) spectroscopy as a tool for pollen identification

Cited by 60 publications

References 23 publications

Primary biological aerosol particles in the atmosphere: a review

Primary biological aerosol particles in the atmosphere: a review

Identification of birch pollen species using FTIR spectroscopy

FTIR analysis of molecular composition changes in hazel pollen from unpolluted and urbanized areas

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