Use of Fluorescent Microspheres in Studies of the Effects of Inhaled Radioactive Materials on the Phagocytic Competence of Alveolar Macrophages

Morgan, A.; Talbot, R.J.; Taya, Akira

doi:10.1093/rpd/38.1-3.205

Cited by 2 publications

(8 citation statements)

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“…There appeared to be no systematic difference in the labeling indices of A M recovered in washes 1-2 and 3-10. This is contrary to the results of similar studies in mice (Morgan et al, 1991 1. In that case not only was the LI greater in the initial washes, but the values of FPM/LAM were also greater.…”

Section: Disc Usslo Ncontrasting

confidence: 99%

“…Following exposure for 1 h, however, 25% of LAM contain 2 or more FPM, and therefore there is a falling off in LI. An almost identical pattern was observed in the mouse (Morgan et al, 1991).…”

Section: Disc Usslo Nsupporting

confidence: 75%

“…Following the administration of slightly smaller FPM (diameter 0.89 pm) to mice by inhalation, their distribution in alveolar macrophages could be fitted in the same way. In that case, however, the geometric standard deviation was about 3 (Morgan et al, 1991). If there is a random association between FPM and AM, the FPMILAM profile would have a binomial distribution.…”

Section: Disc Usslo Nmentioning

confidence: 85%

“…Monodisperse fluorescent polystyrene microspheres (FPM) have been administered to mice by inhalation in order to study the effects of radiation on the phagocytic competence of murine alveolar macrophages (Morgan et al, 1991), and the same workers used a similar technique in order to obtain information on the kinetics of murine alveolar macrophages (Talbot, in preparation). It is intended to use similar methods to study the kinetics of rat alveolar macrophages and, in the present preliminary study, rats were exposed to FPM by inhalation in order to relate the conditions of exposure to the proportion of labeled alveolar macrophages (labeling index, LI) and the distribution of FPM within the labeled A M population.…”

Section: A Morgan Et Al 256mentioning

confidence: 99%

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Labeling Rat Alveolar Macrophages with Fluorescent Microspheres

Morgan

Keilington

Morris

et al. 1995

Inhalation Toxicology

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I Alan HodgsonNational Radiological Protection Board, Chilton, Oxfordshire, United Kingdom Rats were exposed for various periods (5, 20, 60, and 720 min) to an aerosol of fluorescent polystyrene microspheres (FPM) with nominal diameter 7.07 p m . All the animals were killed 40 h later and the lungs were lavaged 70 times with physiological saline. Washings 7-2 and 3-70 were combined. The numbers of alveolar macrophages (AM) recovered by lavage were measured with a Coulter counter. Cytospin slides of cells in the lavage fluid were prepared and used to determine the fraction of recovered AM that contained FPM (labeling index, LO and the FPMIAM profile. The same parameters were also measured by flow cytometry. There was good agreement between determinations of LI and FPMIAM by manual scoring methods and by flow cytometry. Samples of lavage fluid were digested with sodium hypochlorite (bleach), and aliquots of the resulting digests were filtered. Examination of the filters with epifluorescence microscopy enabled the total numbers of FPM present in the various lung washes to be determined. The lavaged lungs were also digested with bleach, and the number of FPM remaining was estimated by manual scoring only. Flow cytometry could not be used for this purpose, due to the presence of cellular debris. After exposure to airborne FPM at the concentration used for 2 h, less than half the recovered AM contained FPM. For studies of AM kinetics it will be necessary to achieve higher LI values and numbers of FPM per cell than were obtained in the present study.

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Section: Disc Usslo Ncontrasting

confidence: 99%

Section: Disc Usslo Nsupporting

confidence: 75%

Section: Disc Usslo Nmentioning

confidence: 85%

Section: A Morgan Et Al 256mentioning

confidence: 99%

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Labeling Rat Alveolar Macrophages with Fluorescent Microspheres

Morgan

Keilington

Morris

et al. 1995

Inhalation Toxicology

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“…These include investigations of the uptake of particles by alveolar macrophages in vitro (Mueller & Guilmette, 1987a) and in vivo following administration either by inhalation (Mueller & Guilmette, 1987b;Morgan et al, 1991Morgan et al, , 1995 or by intratracheal instillation (Takahashi et al, 1992). They have also been used in studies of the clearance of particles from the lung (Velasquez & Morrow, 1984;Lehnert, 1992) and from the pleural cavity (Lehnert et al, 1988).…”

mentioning

confidence: 99%

Are Macrophages Recovered from Mouse Lung by Bronchoalveolar Lavage Representative of the Alveolar Macrophage Pool?

Talbot¹,

Kellington²,

Morgan³

1996

Inhalation Toxicology

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The purpose of this study was to establish whether macrophages recovered from the mouse lung by bronchoalveolar lavage are representative of the alveolar macrophage pool.To this end, mice were exposed by inhalation to an aerosol of monodisperse fluorescent particles (FP) with an actual diameter of 0.89 f i m . Groups of animals were killed at 3 and 27 h following exposure and their lungs were lavaged 30 times in situ with normal saline.After excising the lungs, five additional washes were performed with massage to increase the recovery of free cells. The mean total cell recovery was 0.8 x 106-about half the estimated total number of alveolar macrophages in this strain of mouse. The washes were grouped and analyzed using a fluorescence-activated cell sorter (FACS) to give information on the relative concentrations of (a) free FP, (b) macrophage-associated FP, and (c) unlabeled macrophages. Further, for both free and macrophage-associated FP, the fluorescent events were classified as being due to one, two to three, or greater than or equal to four FP. At both time points a higher proportion of AM in the initial washes contained one or more FP and the numbers of FP per labeled AM were also greater than in subsequent washes. The results of this study suggest that representative samples of the general alveolar macrophage population of the mouse lung can be obtained by bronchopulmonary tavage, provided the initial washes are discarded. It was also apparent from the results that free FP were recovered from the lung much more readily than macrophage-associated FP.

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Use of Fluorescent Microspheres in Studies of the Effects of Inhaled Radioactive Materials on the Phagocytic Competence of Alveolar Macrophages

Cited by 2 publications

References 0 publications

Labeling Rat Alveolar Macrophages with Fluorescent Microspheres

Labeling Rat Alveolar Macrophages with Fluorescent Microspheres

Are Macrophages Recovered from Mouse Lung by Bronchoalveolar Lavage Representative of the Alveolar Macrophage Pool?

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