Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

Mo, Dongqing; Shen, Jie; Zhang, Junzheng

doi:10.1007/978-1-0716-2201-8_4

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…mRpL4 regulates Notch signaling activity in the Drosophila wing During a somatic mosaic screen (Mo et al, 2022), one of the Bruinfly mutant stocks, mRpL4 K14608 , was found to cause marginal defects when homozygous clones were generated in the wings (Fig 1A). As this phenotype is reminiscent of impaired Notch signaling, we examined the expression level of Notch target genes Cut and Wingless (Wg).…”

Section: Resultsmentioning

confidence: 99%

The mitochondrial ribosomal protein mRpL4 regulates Notch signaling

Liu

Chen

et al. 2023

EMBO Reports

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Mitochondrial ribosomal proteins (MRPs) assemble as specialized ribosome to synthesize mtDNA‐encoded proteins, which are essential for mitochondrial bioenergetic and metabolic processes. MRPs are required for fundamental cellular activities during animal development, but their roles beyond mitochondrial protein translation are poorly understood. Here, we report a conserved role of the mitochondrial ribosomal protein L4 (mRpL4) in Notch signaling. Genetic analyses demonstrate that mRpL4 is required in the Notch signal‐receiving cells to permit target gene transcription during Drosophila wing development. We find that mRpL4 physically and genetically interacts with the WD40 repeat protein wap and activates the transcription of Notch signaling targets. We show that human mRpL4 is capable of replacing fly mRpL4 during wing development. Furthermore, knockout of mRpL4 in zebrafish leads to downregulated expression of Notch signaling components. Thus, we have discovered a previously unknown function of mRpL4 during animal development.

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Section: Resultsmentioning

confidence: 99%

The mitochondrial ribosomal protein mRpL4 regulates Notch signaling

Liu

Chen

et al. 2023

EMBO Reports

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“…It has become evident that Notch functions at different developmental stages and within multiple tissues in the fruit fly, sometimes employing distinct modes of action within a single tissue. Systematic in vivo screens have identified extensive sets of genes that are involved in Notch signaling within diverse developmental contexts [ 40 , 41 , 42 , 43 , 44 , 45 ]. The exploration of the molecular functions of these genes will shed light on how Notch signaling accomplishes such sophisticated roles.…”

Section: A Brief Historical Review Of Notch Signaling Studies In ...mentioning

confidence: 99%

Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions

Chen,

Li,

et al. 2023

IJMS

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Notch signaling is an evolutionarily conserved pathway which functions between adjacent cells to establish their distinct identities. Despite operating in a simple mechanism, Notch signaling plays remarkably diverse roles in development to regulate cell fate determination, organ growth and tissue patterning. While initially discovered and characterized in the model insect Drosophila melanogaster, recent studies across various insect species have revealed the broad involvement of Notch signaling in shaping insect tissues. This review focuses on providing a comprehensive picture regarding the roles of the Notch pathway in insect development. The roles of Notch in the formation and patterning of the insect embryo, wing, leg, ovary and several specific structures, as well as in physiological responses, are summarized. These results are discussed within the developmental context, aiming to deepen our understanding of the diversified functions of the Notch signaling pathway in different insect species.

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Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

Cited by 2 publications

References 27 publications

The mitochondrial ribosomal protein mRpL4 regulates Notch signaling

The mitochondrial ribosomal protein mRpL4 regulates Notch signaling

Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions

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