Use of extractable adenosine triphosphate to estimate the viable cell mass in dental plaque samples obtained from monkeys

Robrish, Stanley A.; Kemp, Christopher W.; Bowen, William H.

doi:10.1128/aem.35.4.743-749.1978

Cited by 22 publications

(4 citation statements)

References 20 publications

(16 reference statements)

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“…Intracellular pools of ATP were assessed by mean of standard ATP bioluminescence assays described by Robrish et al . (1978).…”

Section: Methodsmentioning

confidence: 99%

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Co-operative inhibition by fluoride and zinc of glucosyl transferase production and polysaccharide synthesis by mutans streptococci in suspension cultures and biofilms

Koo¹,

Sheng²,

Nguyen³

et al. 2006

FEMS Microbiology Letters

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Fluoride and zinc, alone or in combination at concentrations of 0.2 mM, inhibited production-secretion of glucosyltranferases by Streptococcus mutans UA159 growing in suspension cultures. Inhibition did not involve growth inhibition or starvation. Fluoride and zinc also inhibited glucan production, especially insoluble glucan, in fed-batch biofilms. Inhibition of biofilms appeared to be associated with starvation as indicated by markedly decreased ATP pools and iodophilic polysaccharide levels in biofilm cells. As insoluble glucans are important for virulence of mutans streptococci, the inhibitory actions of fluoride and zinc could significantly affect cariogenicity.

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“…Intracellular pools of ATP were assessed by mean of standard ATP bioluminescence assays described by Robrish et al . (1978).…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular pools of ATP were assessed by mean of standard ATP bioluminescence assays described by Robrish et al (1978). Briefly, ATP was first extracted from samples with boiling Tris-HCl buffer (20 mM Tris-HCl, pH 7.75, plus 2 mM ethylenediamine tetra-acetic acid) maintained at 100 1C for 1.5 min.…”

Section: Determinations Of Atp Poolsmentioning

confidence: 99%

Co-operative inhibition by fluoride and zinc of glucosyl transferase production and polysaccharide synthesis by mutans streptococci in suspension cultures and biofilms

Koo¹,

Sheng²,

Nguyen³

et al. 2006

FEMS Microbiology Letters

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“…Many methods for studying bacterial adhesion have been used , which include direct microscopic counts after suitable staining, flow cytometry and image analysis of fluorescent labelled organisms (Clyne et al, 1997;Grivet et al, 1999), haemagglutination (Goldhar, 1995), attachment to immobilised molecules on thin-layer chromatography plates (Saitoh et al, 1991), radiolabelling (Mackowiak et al, 1984), ATP quantification (Robrish et al, 1977), and immunological methods (Ofek et al, 1995). Although valuable, these studies are artificial as they do not mimic tissue very closely and the cells may not be representative of those occurring in the target tissue.…”

Section: Introductionmentioning

confidence: 99%

Comparison of image analysis software packages in the assessment of adhesion of microorganisms to mucosal epithelium using confocal laser scanning microscopy

O’Mahony

Basset

Holton

et al. 2005

Journal of Microbiological Methods

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We have compared current image analysis software packages in order to find the most useful one for assessing microbial adhesion and inhibition of adhesion to tissue sections. We have used organisms of different sizes, the bacterium Helicobacter pylori and the yeast Candida albicans. Adhesion of FITC-labelled H. pylori and C. albicans was assessed by confocal microscopy. Four different Image analysis software packages, NIH-Image, IP Lab, Image Pro+ and Metamorph, were compared for their ability to quantify adhesion of the two organisms and several quantification methods were devised for each package. For both organisms the dynamic range that could be detected by the software packages was 1 x 10 6 -1 x 10 9 cells/ml. Of the four software packages tested, our results showed that Metamorph software, using our 'Region of Interest' method, with the software's 'Standard Area Method' of counting, was the most suitable for quantifying adhesion of both organisms because of its unique ability to separate clumps of microbial cells. Moreover, fewer steps were required. By preincubating H. pylori with the glycoconjugate Lewis b-HSA, an inhibition of binding of 48.8% was achieved using 250ug/ml Lewis b-HSA. The method we have devised using Metamorph software, provides a simple, quick and accurate way of quantifying adhesion and inhibition of adhesion of microbial cells to the epithelial surface of tissue sections. The method can be applied to organisms ranging in size from small bacteria to larger yeast cells.

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“…A more simple and potentially more accurate and sensitive method for determining cell numbers is to measure extractable ATP using the firefly bioluminescence system (Harber, 1982). Indeed, this technique has already been used successfully to determine viable cell mass in dental plaque (Robrish et al, 1978;Distler et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

A Rapid Bioluminescence Method for Quantifying Bacterial Adhesion to Polystyrene

1983

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Bioluminescence ATP analysis has been used to assess bacterial adhesion with hydrophobic polystyrene tubes as the attachment surface. The assay was performed at 37 degrees C and pH 6.8 with a 10 min incubation period. A variation of more than 200-fold was observed in the adherence capacity of 34 urinary isolates of Escherichia coli, and organisms could be classified as strongly or weakly adherent. All strains capable of strong adhesion possessed both type 1 fimbriae and flagella, and maximum adhesion was expressed during the exponential growth phase. Attachment was in all cases virtually eliminated by addition of 2.5% (w/v) D-mannose to the incubation buffer. Conversely, strains which were deficient in type 1 fimbriae or flagella, or both, were weakly adherent during all phases of growth. There was no correlation between adherence of E. coli to polystyrene and adherence to buccal or uroepithelial cells, but there was a significant association with adherence to uromucoid (P less than 0.002).

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Use of extractable adenosine triphosphate to estimate the viable cell mass in dental plaque samples obtained from monkeys

Cited by 22 publications

References 20 publications

Co-operative inhibition by fluoride and zinc of glucosyl transferase production and polysaccharide synthesis by mutans streptococci in suspension cultures and biofilms

Co-operative inhibition by fluoride and zinc of glucosyl transferase production and polysaccharide synthesis by mutans streptococci in suspension cultures and biofilms

Comparison of image analysis software packages in the assessment of adhesion of microorganisms to mucosal epithelium using confocal laser scanning microscopy

A Rapid Bioluminescence Method for Quantifying Bacterial Adhesion to Polystyrene

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