Use of exploratory factor analysis to ascertain the correlation between the activities of rheumatoid arthritis and infection by human parvovirus B19

Kakurina, Natalja; Kadisa, A.; Lejnieks, Aivars; Mikažāne, Helena; Kozireva, Svetlana; Murovska, Modra

doi:10.1016/j.medici.2015.01.004

Cited by 12 publications

(7 citation statements)

References 16 publications

(16 reference statements)

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“…they have persistent infection in the active phase. Our data coincide with previously published data, in showing that the prevalence of B19V DNA in serum, plasma or peripheral blood cells was significantly higher in patients with RA than in controls (Caliskan et al , 2005; Chen et al , 2006; Kozireva et al , 2008; Tzang et al , 2009b) and that RA activity was higher in patients with latent/persistent B19V infection (Kakurina et al , 2015). However, some authors concluded that B19V is not involved in the aetiopathogenesis of RA (Kerr et al , 1995; Nikkari et al , 1995).…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, it is most likely that the B19V DNA detected in whole blood represents viral DNA from B19V containing blood cells. Moreover, the presence of B19V DNA in peripheral blood cells has been shown previously by Kakurina et al (2015), while the presence of VP1 protein in synovial cells including lymphocytes, macrophages and neutrophils (e.g. cells present in the blood) was shown by Takahashi et al (1998).…”

Section: Discussionmentioning

confidence: 72%

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Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

Naciute

Mieliauskaitė

Rugienė

et al. 2016

Journal of General Virology

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The present study aims to clarify the possible involvement of parvovirus B19 (B19V) infection in rheumatoid arthritis (RA) pathogenesis by investigating the presence of B19V infection markers (genomic sequences and virus-specific antibodies) in association with the level of cytokines and RA clinical activity and aggressiveness. A total of 118 RA patients and 49 age- and sex-matched healthy volunteers were enrolled in the study. Nested PCR was used to detect B19V sequences in whole blood and cell-free plasma DNA, ELISA to detect virus-specific antibodies and cytokine levels in plasma and recomLine dot blot assay for antibodies to separate B19V antigens. The detection frequency of B19V DNA was higher in patients with RA (25.4 %) in comparison with healthy persons (18.4 %). B19V DNA in cell-free plasma (B19+p) was detected significantly often in RA patients in comparison with healthy controls (13.6 vs 2 %; P=0.0002). RA B19+p patients had higher disease activity and aggressiveness, decreased haemoglobin and increased erythrocyte sedimentation rates. IL-6 plasma levels were significantly higher in RA patients than in controls. Within the RA patients’ group the IL-6 level was significantly increased in B19+p patients with disease activity scores of DAS28>5.2, high C-reactive protein and low haemoglobin. Contrary to the healthy controls, the majority of RA B19+p patients did not have antibodies to VP-1S (VP1u) and VP-N (N-terminal half of structural proteins VP1 and VP2), which correspond to the epitopes of neutralizing antibodies. These results indicate that B19V infection at least in some patients is involved in RA pathogenesis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 72%

Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

Naciute

Mieliauskaitė

Rugienė

et al. 2016

Journal of General Virology

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“…The highest level of RA disease activity occurred in patients with active B19 infection 95. In conclusion, there is significant evidence that parvovirus B19 may play a role in the pathogenesis of RA.…”

Section: Autoimmune Diseases Associated With Parvovirus B19 Infectionmentioning

confidence: 80%

The role of parvovirus B19 in the pathogenesis of autoimmunity and autoimmune disease

Kerr

2015

J Clin Pathol

106

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Human parvovirus B19 is a single-stranded DNA virus which preferentially targets the erythroblasts in the bone marrow. B19 infection commonly causes erythema infectiosum, arthralgia, fetal death, transient aplastic crisis in patients with shortened red cell survival, and persistent infection in people who are immunocompromised. Less common clinical manifestations include atypical skin rashes, neurological syndromes, cardiac syndromes, and various cytopenias. B19 infection has also been associated with development of a variety of different autoimmune diseases, including rheumatological, neurological, neuromuscular, cardiovascular, haematological, nephrological and metabolic. Production of a variety of autoantibodies has been demonstrated to occur during B19 infection and these have been shown to be key to the pathogenesis of the particular disease process in a significant number of cases, for example, production of rheumatoid factor in cases of B19-associated rheumatoid arthritis and production of anti-glutamic acid decarboxylase (GAD) in patients with B19-associated type 1 diabetes mellitus. B19 infection has also been associated with the development of multiple autoimmune diseases in 12 individuals. Documented mechanisms in B19-associated autoimmunity include molecular mimicry (IgG antibody to B19 proteins has been shown to cross react with a variety of recognised human autoantigens, including collagen II, keratin, angiotensin II type 1 receptor, myelin basic protein, cardiolipin, and platelet membrane glycoprotein IIb/IIIa), B19-induced apoptosis with presentation of self-antigens to T lymphocytes, and the phospholipase activity of the B19 unique VP1 protein.

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“…Association of B19 infection with RA has been demonstrated in several studies, while B19 specific cellular immune response in these patients was unknown (Takahashi et al, 1998;Murai et al, 1999;Stahl et al, 2000;Kozireva et al, 2008;Kakurina et al, 2015). Although B19 specific cellular immune responses have been investigated very poorly, the existing studies indicate the important role of these responses in the control of acute and persistent B19 infection (Fransilla et al, 2001;Tolfvenstam et al, 2001;Isa et al, 2005;Norbeck et al, 2005;Isa et al, 2006;Streitz et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Peripheral Blood Mononuclear Cells’ Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritis / Reimatoīdā Artrīta Slimnieku Perifēro Asiņu Mononukleāro Šūnu Proliferativā Atbilde Uz Cilvēka Parvovīrusa B19 Antigēniem

Bratslavska

Kozireva

Kadisa

et al. 2016

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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This study aimed to determine peripheral blood mononuclear cells’ (PBMC) proliferative response to parvovirus B19 (B19) antigens in rheumatoid arthritis (RA) patients and possible changes in proliferative response due to chemotherapy. Serum and blood samples of 52 RA patients and 25 sex and age matched healthy individuals were examined for the presence of anti-B19 IgG and IgM class antibodies and virus specific DNA sequence by the recomLine B19 test and nested polymerase chain reaction, respectively. The PBMC proliferative activity was estimated on the 3rd and 6th day of PBMC cultivation in the presence of virus and B19 VP1/VP2 peptide, using thymidine incorporation assay. On the 3rd day, PBMC response to B19 antigens was detected in 74.1% RA patients with active, in 44.8% - with remote and in 40% - with latent stage of persistent B19 infection, while in the control group the response was observed only in two individuals with active viral infection. On the 6th day, the response was found in 50% RA patients with active, 68.9% - with remote and in 80% - with latent stage of latent persistent infection as well as in 41.1% remotely infected control individuals. The highest PBMC mean stimulation indices were detected in the RA patients and control persons with active infection as well as in RA patients with latent stage of persistent viral infection. On the 3rd and 6th day, strong proliferative response was significantly more frequently observed in RA patients not receiving methotrexate treatment, compared to the patients receiving methotrexate treatment in different combinations with other drugs. RA patients had more frequent and faster response to B19 antigens than apparently healthy persons.

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Use of exploratory factor analysis to ascertain the correlation between the activities of rheumatoid arthritis and infection by human parvovirus B19

Abstract: Using EFA and DAS28, a correlation between the clinical activity of RA and B19 infection was confirmed. These data suggest that EFA is applicable for medico-biological studies.

Cited by 12 publications

References 16 publications

Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

The role of parvovirus B19 in the pathogenesis of autoimmunity and autoimmune disease

Peripheral Blood Mononuclear Cells’ Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritis / Reimatoīdā Artrīta Slimnieku Perifēro Asiņu Mononukleāro Šūnu Proliferativā Atbilde Uz Cilvēka Parvovīrusa B19 Antigēniem

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