Use of ex vivo and in vitro cultures of the human respiratory tract to study the tropism and host responses of highly pathogenic avian influenza A (H5N1) and other influenza viruses

Chan, Renee W. Y.; Chan, Michael Chi Wai; Nicholls, John M.; Peiris, J. S. Malik

doi:10.1016/j.virusres.2013.03.003

Cited by 42 publications

(53 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3, S16) (Chan et al 2013). We attributed the post-NA treatment MAA I signal to the non-sialic acid moieties to which this lectin also binds (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…We examined our tissues and cells for α2,3 linkage N-acetylneuraminic (sialic) acids with Maackia amurensis and, for α2,6 linkages with Sambucus nigra (SNA) (Chan et al 2013;Nicholls et al 2007). Maackia amurensis leukoagglutinin (MAA I or MAL) preferably binds Siaα2-3Galβ1-4GlcNAc in N-linked or core 2 O-linked sialic acids as well as unsialylated glycan, SO 4 -3-Galβ1-4GlcNAc (Geisler and Jarvis 2011).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea

Davis

Chertow

Moyer

et al. 2015

J Histochem Cytochem.

View full text Add to dashboard Cite

Primary normal human bronchial/tracheal epithelial (NHBE) cells, derived from the distal-most aspect of the trachea at the bifurcation, have been used for a number of studies in respiratory disease research. Differences between the source tissue and the differentiated primary cells may impact infection studies based on this model. Therefore, we examined how well-differentiated NHBE cells compared with their source tissue, the human distal trachea, as well as the ramifications of these differences on influenza A viral pathogenesis research using this model. We employed a histological analysis including morphological measurements, electron microscopy, multi-label immunofluorescence confocal microscopy, lectin histochemistry, and microarray expression analysis to compare differentiated NHBEs to human distal tracheal epithelium. Pseudostratified epithelial height, cell type variety and distribution varied significantly. Electron microscopy confirmed differences in cellular attachment and paracellular junctions. Influenza receptor lectin histochemistry revealed that α2,3 sialic acids were rarely present on the apical aspect of the differentiated NHBE cells, but were present in low numbers in the distal trachea. We bound fluorochrome bioconjugated virus to respiratory tissue and NHBE cells and infected NHBE cells with human influenza A viruses. Both indicated that the pattern of infection progression in these cells correlated with autopsy studies of fatal cases from the 2009 pandemic.

show abstract

“…3, S16) (Chan et al 2013). We attributed the post-NA treatment MAA I signal to the non-sialic acid moieties to which this lectin also binds (Fig.…”

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea

Davis

Chertow

Moyer

et al. 2015

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…The PCL, from which soluble mucins are excluded, is formed by the cilia and microvilli that protrude from the membranes of ciliated cells, and the mucin-secreting goblet cells and serous cells, respectively [104]. These are the major cell types of the mammalian upper respiratory tract that face the mucus layer and of which at least ciliated and goblet cells can be infected by IAVs [105][106][107]. The narrow space (<200 nm) in between the cilia, filled with a glycocalyx composed of sialylated membrane-tethered mucins and other glycoconjugates, is impenetrable for beads >40 nm by diffusion [108].…”

Section: Getting Inmentioning

confidence: 99%

Influenza A Virus Hemagglutinin–Neuraminidase–Receptor Balance: Preserving Virus Motility

Vries

Guo

et al. 2020

Trends in Microbiology

140

138

View full text Add to dashboard Cite

“…It is well known that influenza viruses have high affinity to replicate in epithelial cell layers in vivo in particular the upper and lower respiratory tract of mammals [23,24] and intestinal epithelium of birds [25,26]. In addition, viruses have been successfully cultivated in vitro in epithelial cells extracted from respiratory organs and intestine [27,28].…”

Section: Discussionmentioning

confidence: 99%

Cultivation of Influenza a Virus in Primary Cell Culture of Turkey Embryonic Fibroblasts

Al-Mubarak¹

2017

Bas.J.Vet.Res.

View full text Add to dashboard Cite

Cell cultures in particular the clonally selected Madin Darby Canine Kidney (MDCK) cell lines are widely used to cultivate influenza viruses because of their high susceptibility to infection and their ability to produce high number of viruses. However, these cells have been in culture for decades and are well adapted to the two-dimensional culture environment, and as a result, often differ genetically, physiologically, and phenotypically from their tissue origin. The aim of this study was to extract turkey embryonic fibroblast cells directly from tissue as a new primary cell type and then infect them with H2N3 influenza A virus to determine their susceptibility to infection. This cell type will have normal cell characters and maintain many of the important markers and functions seen in vivo. Results showed that the level of susceptibility to infection was comparable between turkey embryonic fibroblasts and MDCK cell line based on incubating with peroxidase labelled monoclonal IgG antibody to viral nucleoprotein. In addition, progeny virions were clearly visualized on the surface of turkey embryonic fibroblasts by using transmission electron microscope. For further confirmation, progeny virions were also detected in the infected cells following treatment with a fluorescently labelled IgG antibody specific to viral H2 protein by performing immunofluorescent technique. This study confirms that turkey embryonic fibroblast cells are susceptible to infection with influenza viruses and can be considered as a primary cell model to cultivate influenza viruses and to study their effects on cells.

show abstract

Use of ex vivo and in vitro cultures of the human respiratory tract to study the tropism and host responses of highly pathogenic avian influenza A (H5N1) and other influenza viruses

Cited by 42 publications

References 131 publications

Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea

Validation of Normal Human Bronchial Epithelial Cells as a Model for Influenza A Infections in Human Distal Trachea

Influenza A Virus Hemagglutinin–Neuraminidase–Receptor Balance: Preserving Virus Motility

Cultivation of Influenza a Virus in Primary Cell Culture of Turkey Embryonic Fibroblasts

Contact Info

Product

Resources

About