Use of Environmental DNA to Detect Grass Carp Spawning Events

Hayer, Cari‐Ann; Bayless, Michael F.; George, Amy E.; Thompson, Nathan L.; Richter, Catherine A.; Chapman, Duane

doi:10.3390/fishes5030027

Cited by 15 publications

(18 citation statements)

References 22 publications

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“…This peak matches the reproduction period of most of the lacustrine species detected, suggesting that species phenology plays a major role in the temporal variability of eDNA signal. eDNA concentration followed a strong seasonal variation, with a peak of eDNA concentration or number of reads observed during fish reproduction [88,89]. This was also observed with metabarcoding data for late autumn/winter spawners, such as Coregonus lavaretus.…”

Section: Plos Onesupporting

confidence: 73%

Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes

et al. 2022

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Environmental DNA (eDNA) metabarcoding is revolutionizing the monitoring of aquatic biodiversity. The use of eDNA has the potential to enable non-invasive, cost-effective, time-efficient and high-sensitivity monitoring of fish assemblages. Although the capacity of eDNA metabarcoding to describe fish assemblages is recognised, research efforts are still needed to better assess the spatial and temporal variability of the eDNA signal and to ultimately design an optimal sampling strategy for eDNA monitoring. In this context, we sampled three different lakes (a dam reservoir, a shallow eutrophic lake and a deep oligotrophic lake) every 6 weeks for 1 year. We performed four types of sampling for each lake (integrative sampling of sub-surface water along transects on the left shore, the right shore and above the deepest zone, and point sampling in deeper layers near the lake bottom) to explore the spatial variability of the eDNA signal at the lake scale over a period of 1 year. A metabarcoding approach was applied to analyse the 92 eDNA samples in order to obtain fish species inventories which were compared with traditional fish monitoring methods (standardized gillnet samplings). Several species known to be present in these lakes were only detected by eDNA, confirming the higher sensitivity of this technique in comparison with gillnetting. The eDNA signal varied spatially, with shoreline samples being richer in species than the other samples. Furthermore, deep-water samplings appeared to be non-relevant for regularly mixed lakes, where the eDNA signal was homogeneously distributed. These results also demonstrate a clear temporal variability of the eDNA signal that seems to be related to species phenology, with most of the species detected in spring during the spawning period on shores, but also a peak of detection in winter for salmonid and coregonid species during their reproduction period. These results contribute to our understanding of the spatio-temporal distribution of eDNA in lakes and allow us to provide methodological recommendations regarding where and when to sample eDNA for fish monitoring in lakes.

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Section: Plos Onesupporting

confidence: 73%

Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes

et al. 2022

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“…There are several studies using eDNA that focused on fish spawning (e.g., Erickson et al, 2016;Bylemans et al, 2017;Tillotson et al, 2018;Thalinger et al, 2019;Hayer et al, 2020;Yatsuyanagi et al, 2020). Thalinger et al (2019) and Yatsuyanagi et al (2020) showed that eDNA concentrations increased because of fish migration into rivers during the spawning season.…”

Section: Introductionmentioning

confidence: 99%

“…Thalinger et al (2019) and Yatsuyanagi et al (2020) showed that eDNA concentrations increased because of fish migration into rivers during the spawning season. Erickson et al (2016); Tillotson et al (2018), andHayer et al (2020) reported that fish spawning behavior in rivers could temporarily increase eDNA concentrations and the spawning behavior could be captured using eDNA. These studies show that eDNA may increase with fish spawning behavior, perhaps because DNA fragments from sperm and dead fish promote a temporary increase in the eDNA concentrations (Bylemans et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Changes of the Environmental DNA Concentrations of Amphidromous Fish Plecoglossus altivelis altivelis in the Spawning Grounds in the Takatsu River, Western Japan

et al. 2021

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The Ayu Plecoglossus altivelis altivelis is an amphidromous fish that is not only the most important commercial fishery species in Japanese rivers but also has a high economic value in recreational fishing. However, the degradation of its spawning grounds has caused a decrease in its abundance. In this study, we used environmental DNA (eDNA) to monitor the Ayu in the Takatsu River in Japan to (1) identify the spawning season in three known spawning grounds, (2) clarify changes in the main spawning grounds during the spawning season, and (3) discover unknown spawning grounds. We collected 1 L of the surface river water at three known spawning grounds nine times in 2018 and seven times in 2019 in the lower reaches of the Takatsu River. We also collected samples from seven unknown sites in 2018. The water samples were filtered through glass fiber filters. Total eDNA was extracted from each filtered sample and a Real-time quantitative PCR was performed with the specific primers and probe for Ayu. The results of the eDNA analyses showed that (1) the spawning season was in November in 2018 and in September in 2019. (2) One site was used as a spawning ground in both the early and the late spawning season, depending on the year. At the second site, the frequency of use changed year by year. The third site was the main spawning ground in the middle to late spawning season every year. From these results, we elucidated that some spawning grounds are used regularly every year, while the use of others varies year by year. (3) In five of the seven unknown sites, the nighttime eDNA concentrations were high at least once during the four surveys, suggesting that these sites may have functioned as spawning grounds. In particular, one site could be an important new spawning ground.

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“…There are several reviews of this rapidly developing topic [9]. In this volume, Hayer and colleagues [10] describe how eDNA presence in river waters shows a positive relationship to the presence of invasive grass carp, Ctenopharyngodon idella, in North America, especially when these fish are spawning. Because, grass carp, like many invasive fishes, are very difficult to sample, this is an important observation, especially if eDNA concentration could be directly linked to reproductive state.…”

Section: Techniques To Assess the Presence And Distribution Of Invasi...mentioning

confidence: 99%

Introduction to the Biology and Control of Invasive Fishes and a Special Issue on This Topic

Sorensen

2021

Fishes

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Across the globe, dozens of species of invasive fish are now found in fresh as well as marine waters, where they alter habitats, compete with native fish for food, and prey on native fishes, exerting both indirect and direct effects on ecosystems and economies. While efforts to understand and control these species are growing, most are still in their infancy; however, a few examples stand out. This special issue is comprised of 11 notable articles on freshwater invasive fish and is the first to address this topic. This introductory article serves as an introduction to these articles which focus on 5 topics on invasive freshwater fish: (1) the damage they cause (one article); (2) techniques to ascertain their presence (one article); (3) techniques to restrict their movement (one article); (4) strategies to control them (three articles); and (5) lessons learned from ongoing management efforts (five articles). This introduction notes that successful management efforts share a few approaches: (1) they develop and use a deep understanding of local species and their abundance as well as distribution; (2) they focus on reducing reproductive success; (3) they use multiple complimentary control strategies; and (4) they use a long-term approach.

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Use of Environmental DNA to Detect Grass Carp Spawning Events

Cited by 15 publications

References 22 publications

Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes

Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes

Spatiotemporal Changes of the Environmental DNA Concentrations of Amphidromous Fish Plecoglossus altivelis altivelis in the Spawning Grounds in the Takatsu River, Western Japan

Introduction to the Biology and Control of Invasive Fishes and a Special Issue on This Topic

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