Abstract:Proteins of 15 isolates of T. longbrachiatum and T. harzianum were compared by polyacrylamid gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Gels were stained with silver nitrate. Protein banding patterns of the isolates were subjected to cluster analysis by the unweighted pair-group method based on arithmetic mean (UPGMA).
“…However, the polymorphism of plant isozymes is low, resulting in a poor stability. At the same time, this polymorphism is susceptible to environmental factors, sampling locations, and plant development stages [21,22]. Molecular markers have the advantages of a high stability, good repeatability, and wide distribution in the genome [23,24].…”
An electrochemical voltammogram recording method for plant variety identification is proposed. Electrochemical voltammograms of Vistula, Andromeda, Danuta, Armandii ‘Apple Blossom,’ Proteus, Hagley Hybrid, Violet Elizabeth, Kiri Te Kanawa, Regina, and Veronica’s Choice were recorded using leaf extracts with two solvents under buffer solutions. The voltametric data recorded under different conditions were derived as scatter plots, 2D density patterns, and hot maps for variety identification. In addition, the voltametric data were further used for genetic relationship studies. The dendrogram deduced from the voltammograms was used as evidence for relationship study. The dendrogram deduced from voltametric data suggested the Andromeda, Danuta, Proteus, Regina, and Hagley Hybrid were closely related, while Violet Elizabeth and Veronica’s Choice were closely related. In addition, Vistula and Armandii ‘Apple Blossom’ could be considered outliers among the varieties.
“…However, the polymorphism of plant isozymes is low, resulting in a poor stability. At the same time, this polymorphism is susceptible to environmental factors, sampling locations, and plant development stages [21,22]. Molecular markers have the advantages of a high stability, good repeatability, and wide distribution in the genome [23,24].…”
An electrochemical voltammogram recording method for plant variety identification is proposed. Electrochemical voltammograms of Vistula, Andromeda, Danuta, Armandii ‘Apple Blossom,’ Proteus, Hagley Hybrid, Violet Elizabeth, Kiri Te Kanawa, Regina, and Veronica’s Choice were recorded using leaf extracts with two solvents under buffer solutions. The voltametric data recorded under different conditions were derived as scatter plots, 2D density patterns, and hot maps for variety identification. In addition, the voltametric data were further used for genetic relationship studies. The dendrogram deduced from the voltammograms was used as evidence for relationship study. The dendrogram deduced from voltametric data suggested the Andromeda, Danuta, Proteus, Regina, and Hagley Hybrid were closely related, while Violet Elizabeth and Veronica’s Choice were closely related. In addition, Vistula and Armandii ‘Apple Blossom’ could be considered outliers among the varieties.
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