2006
DOI: 10.1128/jcm.44.2.561-570.2006
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Use of Electrochemical DNA Biosensors for Rapid Molecular Identification of Uropathogens in Clinical Urine Specimens

Abstract: We describe the first species-specific detection of bacterial pathogens in human clinical fluid samples using a microfabricated electrochemical sensor array. Each of the 16 sensors in the array consisted of three single-layer gold electrodes-working, reference, and auxiliary. Each of the working electrodes contained one representative from a library of capture probes, each specific for a clinically relevant bacterial urinary pathogen. The library included probes for Escherichia coli, Proteus mirabilis, Pseudom… Show more

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Cited by 193 publications
(209 citation statements)
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“…Th e practical utility of this new motion-driven DNA assay was illustrated using the same capture and detector probe for the detection of 16S rRNA which is released from E. coli pathogenic bacteria and obtained from a previously reported sample preparation 20,21 . Figure 3b and the corresponding Supplementary Movie 4 show catalytic nanomotors ' racing ' following hybridization assays using diff erent bacterial lysate solutions corresponding to diff erent E. coli cell concentrations: 0, 7 × 10 3 and 7 × 10 5 CFU μ l − 1 , leading to average distance signals of 32, 56 and 96 μ m, respectively ( Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Th e practical utility of this new motion-driven DNA assay was illustrated using the same capture and detector probe for the detection of 16S rRNA which is released from E. coli pathogenic bacteria and obtained from a previously reported sample preparation 20,21 . Figure 3b and the corresponding Supplementary Movie 4 show catalytic nanomotors ' racing ' following hybridization assays using diff erent bacterial lysate solutions corresponding to diff erent E. coli cell concentrations: 0, 7 × 10 3 and 7 × 10 5 CFU μ l − 1 , leading to average distance signals of 32, 56 and 96 μ m, respectively ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Similarly, magnetic Au -Ni -Au -Pt nanomotors were prepared by introducing a ferromagnetic Ni segment. Following an initial deposition of gold segment at 0.75 C, Ni was electrodeposited at − 1.0 V for 2 C (versus Ag / AgCl) from a plating solution (20 4)). Subsequently, the second gold segment (0.75 C) and a platinum segment were electrodeposited as above.…”
Section: Preparation Of Nanomotorsmentioning
confidence: 99%
“…We typically perform the hybridization steps at ambient temperature (~20 °C) 5,6 . However, the hybridization steps (3.2 and 3.3) can also be performed at higher temperatures in a hybridization oven if the chip is placed in a covered chamber containing moistened filter paper to prevent evaporation 7,8 . Optimal signal is obtained with capture-detector probe pairs that hybridize to adjacent sites on the nucleic acid target without a gap between the hybridization sites 8 .…”
Section: Discussionmentioning
confidence: 99%
“…However, little attention has been given to the performance of DNA biosensors in raw biological samples. The electrochemical sensor assay described here can be performed directly on serum and urine samples without a significant loss of sensitivity 7 . The use of thiolated capture probes and the mixed monolayer described here renders the sensor surface resistant to non-specific protein absorption, retaining sensitivity in raw biological specimens including serum or urine 10 .…”
Section: Discussionmentioning
confidence: 99%
“…2 Recent engineering advances have enabled the development of electrochemical DNA biosensors with molecular diagnostic capabilities. 4 Electrochemical DNA biosensors offer several advantages compared to alternative molecular detection approaches, including the ability to analyze complex body fluids, high sensitivity, compatibility with microfabrication technology, a low power requirement, and compact instrumentation compatible with portable devices. [5][6][7] The development of electrochemical transduction schemes for DNA bionsensor (so-called genosensors) has recently received increasing attention using photoelectrochemistry, potentiometry, and DNA-modified electrodes.…”
Section: Introductionmentioning
confidence: 99%