Use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by ELISA

Zrein, Maan; Obert, G.; Regenmortel, M.H.V. Van

doi:10.1007/bf01317370

Cited by 10 publications

(4 citation statements)

References 24 publications

(9 reference statements)

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“…Furthermore, of 42 IFA negative specimens, two were positive by M-ELISA. Similar results have been published for ELISA employing polyclonal mammalian antisera [33,35] or egg-yolk antibodies [39] obtained by immunization with purified nucleocapsid. Additionally, the M-ELISA was found as efficient for antigen detection in nasopharyngeal secretions as the ELISA described by Sarkkinen et al [35] and using polyclonal antibodies against the viral nucleocapsid.…”

Section: Discussionsupporting

confidence: 80%

An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens

Obert

Beyer

1988

Archives of Virology

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An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of respiratory syncytial virus in nasopharyngeal secretions. This assay employed as immunoreagents two monoclonal antibodies directed against two distinct epitopes of the viral nucleocapsid. One of them (RSV 4) was used for antigen capture and the other (NC 4) was labelled with N-hydroxy-succinimide-epsilon-caproil biotin and used for antigen detection. Streptavidin biotin-peroxidase complexes were employed as amplification mode. The immunoassay was performed in 6 hours and was able to detect as little as 1 ng/ml of purified nucleocapsid. When 87 nasopharyngeal secretions were analyzed by an indirect immunofluorescence assay using commercial reagents and by the newly developed ELISA, the sensitivity and the specificity of the two assays were found to be very similar.

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Section: Discussionsupporting

confidence: 80%

An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens

Obert

Beyer

1988

Archives of Virology

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“…It was further elucidated that hen egg yolk contained IgG almost exclusively, while IgA and IgM were selectively located in egg white (32). Since the content of yIgG recovered from egg yolk was usually about 100 mg, eggs from hyperimmunized hens may provide a convenient and economical source of antibodies for passive immunization (39,42,43). Another advantage of yIgG is that collecting eggs from laying hens does not require the bleeding of animals for antiserum production, which is especially suited to current regulations for experimental animal protection.…”

Section: Discussionmentioning

confidence: 99%

Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans

et al. 1991

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The effect of polyclonal egg yolk immunoglobulin G antibodies (yIgG) raised against whole cells, cell-free (CF) glucosyltransferase (GTase), or cell-associated (CA) GTase of serotype c Streptococcus mutans was examined in terms of in vitro inhibition of virulence-related factors of S. mutans and protection of S. mutans-infected rats against the development of dental caries. Hens (18 weeks old) were immunized with formalin-treated whole cells, purified CF-GTase, or CA-GTase together with Freund's complete adjuvant. In addition, yIgG to surface protein antigen was used in some in vitro experiments for comparison with other antibodies. yIgG was purified by ammonium sulfate precipitation followed by DEAE-Sephacel column chromatography or fractional precipitation with ethanol. Purified yIgG was found to be a 220-kDa protein, which was dissociated into heavy and light chains upon addition of 2-mercaptoethanol. yIgG to whole cells and surface protein antigen gave a heavy aggregation of S. mutans organisms, while yIgG to CF-and CA-GTase specifically inhibited the enzymatic activity of the respective GTase. yIgG to CA-GTase and whole cells was found to clearly suppress the adherence of S. mutans cells to a glass surface. Specific-pathogen-free Sprague-Dawley rats that had been infected heavily and repeatedly with S. mutans and fed diet no. 2000 developed severe dental caries, while rats fed diet 2000 containing .0.1% yIgG to CA-GTase showed a statistically significant reduction in dental plaque accumulation and caries development. Administration of yIgG to CF-GTase and whole cells failed to protect against caries. These results clearly suggest that yIgG to S. mutans CA-GTase specifically inhibited a virulence factor of this organism, i.e., insoluble glucan-synthesizing CA-GTase, resulting in a significant reduction in the development of dental caries.

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“…The successful development of efficient methods to isolate immunoglobulins from egg yolk was the basic requirement of a routine application of yolk antibodies in microbiology (21,22,26,29,36), parasitology (8, lo), and biochemistry (4, 5).…”

Section: Introductionmentioning

confidence: 99%

Production of Antibodies to Canine Distemper Virus in Chicken Eggs for Immunohistochemistry

Schmidt

Hafner

Reubel

et al. 1989

Journal of Veterinary Medicine, Series B

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The present study describes the application of egg yolk antibodies in immunohistochemistry. In order to obtain specific antibodies against canine distemper virus (CDV), chickens were immunized with attenuated virus. Distinct antibody titres in serum and yolk could be detected by means of a modified plaque/ focus immunoassay (ELISA) two weeks after a second immunization. The lower concentrations in corresponding yolk globulin preparations are attributed to the loss of antibodies caused by the isolation procedure (dextrane and ammonium sulfate precipitation). After verification of the antibody specificity by indirect immunofluorescence technique high titred globulin fractions were employed in immunohistochemistry using the Avidin-Biotin-Complex method. A specific and distinct immunostaining in formalin fixed and paraffin embedded brain sections of CDV-infected dogs was obtained. The advantages of egg yolk antibodies for immunological purposes are discussed in detail.

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Use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by ELISA

Cited by 10 publications

References 24 publications

An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens

An enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens

Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans

Production of Antibodies to Canine Distemper Virus in Chicken Eggs for Immunohistochemistry

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