Use of Ear Chamber with Uterine Muscle Transplant to differentiate Myometrial and Vascular Activity

Cliff, W. J.; Martin, John D.; Michael, C. A.

doi:10.1038/199399a0

Cited by 11 publications

(3 citation statements)

References 5 publications

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“…A glass-chamber method for the in vivo study of tissue was introduced by E. Ziegler in 1875. The technique was later used for installation of an ear chamber in the rabbit (Sandison 1924) and for the subsequent observation of uterine tissue transplants (Cliff et al 1963). In the present study 21 rabbits had myometrial autografts (0.5-1 .O mm3) placed in pre-inserted bilateral transparent ear-chambers by the technique previously described by Wagner (1975a, b).…”

Section: In Vivo Ear Chamber Experimentsmentioning

confidence: 99%

Vasoactive intestinal polypeptide (VIP): Effect on rabbit uterine smooth muscle in vivo and in vitro

Ottesen

1981

Acta Physiologica Scandinavica

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The effect of vasoactive intestinal polypeptide (VIP) on uterine smooth muscle electrical and mechanical activity in non-pregnant estradiol-treated rabbits was investigated using in vivo and in vitro methods. The studies were performed on spontaneous, oxytocin-, carbachol-, and prostaglandin-42 alpha-induced activity. VIP had a dose-related inhibitory effect on both myoelectrical and mechanical activity. The concentration needed for 50% inhibition (ID50) was 2 x 10(-10) mol VIP . 1(-1) (in vivo), an 6 x 10(-8) mol VIP . 1(-1) (in vitro). This inhibition was unaffected by the presence of atropine (10(-5) mol . 1(-1)), propranolol (10(-5)), phentolamine (10(-5)), naloxone (10(-5)), apamin (10(-5)), and tetrodotoxin (10(-5)). These findings indicate that VIP may act via a specific receptor on the smooth muscle and supports the hypothesis that VIP may be a neurotransmitter involved in the local nervous control of uterine smooth muscle activity.

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Section: In Vivo Ear Chamber Experimentsmentioning

confidence: 99%

Vasoactive intestinal polypeptide (VIP): Effect on rabbit uterine smooth muscle in vivo and in vitro

Ottesen

1981

Acta Physiologica Scandinavica

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“…The introduction of a method of grafting myometrial tissue to a transparent ear chamber (Cliff et al 1964) led to a series of experiments of the function of myometrial grafts (Wagner 1975a,b).…”

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confidence: 99%

Function of Myometrial Graft at Time of Parturition in the Rabbit

Wagner

1975

Acta Endocrinologica

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Thirteen rabbits equipped with ear chambers containing myometrial grafts were studied during late pregnancy, spontaneous and induced parturition and early puerperium. Spontaneous electrical activity of the graft was recorded by means of F.-M. telemetry transmission in the unrestrained animals. Furthermore the reaction to intravenously injected oxytocin (threshold determination) was recorded daily. During the last 5 days before spontaneous delivery increasing spontaneous activity of the tissue was observed. During the same period the response to oxytocin increased. Concomitantly with the delivery of the litter a period of rhythmic activity was observed. This period had a sudden onset and lasted 12\p=n-\55min, while the delivery itself only lasted 6\p=n-\16min. Similar activity patterns could be evoked in the early post-partum period by 100 mU oxytocin given iv. In 3 rabbits, doses from 80\p=n-\300mU oxytocin induced complete delivery. During the 48 hours' period after delivery spontaneous activity (single spikes and series of bursts) was observed. The response to oxytocin was further increased. It is concluded that as the graft is not influenced by nervous regulation, mechanical influence or local humoral factors the spontaneous parturition and the change in excitability must therefore be due to one (or more) systemic, humoral factor(s).In spite of extensive research on the endocrine control of the myometrium and on the electrophysiological and mechanical behaviour of the myometrium under normal and controlled experimental conditions, the physiology of normal parturition remains obscure. The crucial question: what are the essential factors determining the time of onset of labour, has not yet been fully answered in any of the species studied.

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“…The graft in the ear of the rabbit is readily accessible and allows repeated study of a single group of cells for long periods (Cliff, Martin & Michael, 1963). So far no studies of the spontaneous electrical activity of the myometrium have been undertaken in the unrestrained rabbit.…”

Section: Introductionmentioning

confidence: 99%

Electrical activity of grafted myometrium and its recording by radio‐telemetry in unrestrained rabbits.

Wagner¹

1975

The Journal of Physiology

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SUMMARY1. The electrical activity in myometrial grafts in fifty-one female rabbits with ear chambers fitted with pick-up electrodes has been monitored.An FM telemetry system has made long-term studies possible in the freely moving animal.2. Two distinct patterns of electrical activity were observed either arising spontaneously or provoked by oxytocin. These were the 'long. duration burst' containing about 50-150 spikes, and the 'short-duration burst' containing 1-10 spikes.3. The spike frequency within the long-duration bursts varied between 0 9 and 1-3 spikes sec-1 and was fairly constant during the first half of the burst. The frequency of the bursts in the long series of short-duration bursts was about 2-3 min-.4. When activity from grafts in two chambers in the same animal was monitored for long periods, spontaneous activity occurred at random throughout the day in each graft, indicating that the spontaneous activity was probably myogenic.

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Use of Ear Chamber with Uterine Muscle Transplant to differentiate Myometrial and Vascular Activity

Cited by 11 publications

References 5 publications

Vasoactive intestinal polypeptide (VIP): Effect on rabbit uterine smooth muscle in vivo and in vitro

Vasoactive intestinal polypeptide (VIP): Effect on rabbit uterine smooth muscle in vivo and in vitro

Function of Myometrial Graft at Time of Parturition in the Rabbit

Electrical activity of grafted myometrium and its recording by radio‐telemetry in unrestrained rabbits.

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