Use of Dynamic Contact Angle Profile Analysis in Studying the Kinetics of Protein Removal from Steel, Glass, Polytetrafluoroethylene, Polypropylene, Ethylenepropylene Rubber, and Silicone Surfaces

Davies, John A.; Nunnerley, Caroline S.; Brisley, Anneli C.; Edwards, John C.; Finlayson, Stewart D.

doi:10.1006/jcis.1996.0485

Cited by 26 publications

(18 citation statements)

References 2 publications

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“…Possible restructuring effects at the glass interface was eliminated since a solvent‐swollen network (i.e., not a neat film) was produced in the mold, removed and then dried in air. As expected, SMP1–4 are hydrophobic as indicated by θ static values above 90°45 and also higher than that of pure PCL due to the presence of hydrophobic PDMS segments. θ static of SMP4 , perhaps due to the increased crosslink density and PCL crystallinity, was slightly higher than that of the silicone control.…”

Section: Resultssupporting

confidence: 76%

Synergistic action of fibroblast growth factor‐2 and transforming growth factor‐beta1 enhances bioprinted human neocartilage formation

Cui

Breitenkamp

Lotz

et al. 2012

Biotech & Bioengineering

105

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Bioprinting as a promising but unexplored approach for cartilage tissue engineering has the advantages of high throughput, digital control, and highly accurate placement of cells and biomaterial scaffold to the targeted 3D locations with simultaneous polymerization. This study tested feasibility of using bioprinting for cartilage engineering and examined the influence of cell density, growth and differentiation factors. Human articular chondrocytes were printed at various densities, stimulated transiently with growth factors and subsequently with chondrogenic factors. Samples were cultured for up to 4 weeks to evaluate cell proliferation and viability, mechanical properties, mass swelling ratio, water content, gene expression, ECM production, DNA content, and histology. Bioprinted samples treated with FGF-2/TGF-β1 had the best chondrogenic properties among all groups apparently due to synergistic stimulation of cell proliferation and chondrogenic phenotype. ECM production per chondrocyte in low cell density was much higher than that in high cell seeding density. This finding was also verified by mechanical testing and histology. In conclusion, cell seeding density that is feasible for bioprinting also appears optimal for human neocartilage formation when combined with appropriate growth and differentiation factors.

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Section: Resultssupporting

confidence: 76%

Synergistic action of fibroblast growth factor‐2 and transforming growth factor‐beta1 enhances bioprinted human neocartilage formation

Cui

Breitenkamp

Lotz

et al. 2012

Biotech & Bioengineering

105

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“…Surface reorganization may also be characterized via dynamic contact angle analysis. θ adv represents the hydrophobic surface characteristics, whereas hydrophilicity is reflected by θ rec 48. Hysteresis (θ δ = θ adv − θ rec ) is typically used as an indicator of surface reorganization 49.…”

Section: Resultsmentioning

confidence: 99%

Amphiphilic silicones prepared from branched PEO‐silanes with siloxane tethers

Murthy

Bailey

Valentin-Rodriguez

et al. 2010

J. Polym. Sci. A Polym. Chem.

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Amphiphilic silicones were prepared by the covalent incorporation of branched polyethylene oxide (PEO) via a siloxane tether. This was achieved by using six novel branched PEO-silanes with varying siloxane tether lengths and PEO molecular weight (M n ). Each PEO-silane was crosslinked via acid-catalyzed sol-gel condensation with a,x-bis(Si-OH) polydimethylsiloxane (PDMS) (M n ¼ 3000 g/mol) to yield six amphiphilic silicone films. Film surface hydrophilicity increased with siloxane tether length, particularly after exposure to an aqueous environment, indicating that the PEO segments were more readily driven to the surface. This effect was more pronounced for films prepared with PEO-silanes containing lower M n PEO segments. AFM was used to study surface reconstruction of films upon exposure to an aqueous environment. Adsorption of bovine serum albumin (BSA) and human fibrinogen (HF) proteins decreased with siloxane tether length, particularly after first exposing films to an aqueous environment. For a given siloxane tether length, relatively less BSA adsorbed onto films prepared with PEO-silanes with lower M n PEO segments whereas less HF adsorbed onto films prepared with PEO-silanes with higher M n PEO segments. V C 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 4108-4119, 2010

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“…θ adv is an indicator of the hydrophobicity or low surface energy, whereas θ rec reflects the hydrophilic nature of the surface 53. Hysteresis (θ Δ = θ adv − θ rec ) is a product of surface chemical heterogeneity, roughness, reorganization, hydration, or contamination 54.…”

Section: Discussionmentioning

confidence: 99%

Minimally adhesive polymer surfaces prepared from star oligosiloxanes and star oligofluorosiloxanes

Grunlan

Lee

Mansfeld

et al. 2006

J. Polym. Sci. A Polym. Chem.

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The effects of the surface energy, storage modulus (G′), and glass‐transition temperature (Tg) on the biofouling behavior of siloxane and fluorosiloxane polymer surfaces (films) were studied. Irregular SiH‐terminated tetrabranched star oligosiloxanes and star oligofluorosiloxanes were prepared by the acid‐catalyzed equilibration of octamethylcyclotetrasiloxane or 1,3,5‐trimethyl‐1,3,5‐tris(3′,3′,3′‐trifluoropropyl)cyclotrisiloxane with tetrakis(dimethylsiloxy)silane, respectively. Terminal epoxy groups were introduced via Pt‐catalyzed hydrosilylation with allyl glycidyl ether to yield compounds that were subsequently crosslinked with α,ω‐bis(3‐aminopropyl)poly(dimethylsiloxane). The resulting films were characterized by goniometry, dynamic mechanical thermal analysis, and thermogravimetric analysis. The foul‐release behavior was studied by the measurement of how strongly sporelings (young plants) of the green seaweed Ulva adhered. The corrosion protection of aluminum was evaluated by electrochemical impedance spectroscopy. Fluorosiloxane films displayed higher G′ and Tg values, decreased contact angles (with water), and more effectively released Ulva sporelings in comparison with siloxane films. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 2551–2566, 2006

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Use of Dynamic Contact Angle Profile Analysis in Studying the Kinetics of Protein Removal from Steel, Glass, Polytetrafluoroethylene, Polypropylene, Ethylenepropylene Rubber, and Silicone Surfaces

Cited by 26 publications

References 2 publications

Synergistic action of fibroblast growth factor‐2 and transforming growth factor‐beta1 enhances bioprinted human neocartilage formation

Synergistic action of fibroblast growth factor‐2 and transforming growth factor‐beta1 enhances bioprinted human neocartilage formation

Amphiphilic silicones prepared from branched PEO‐silanes with siloxane tethers

Minimally adhesive polymer surfaces prepared from star oligosiloxanes and star oligofluorosiloxanes

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