Use of different Taq DNA polymerases for detection of Chlamydia trachomatis in cervical samples

Oliveira, Francisca Laís Araújo de; Azevedo, M. J.; Barbosa, Anderson Nogueira; Rodrigues, Ricardo Carvalho; Reis, Renato dos Santos; Rocha, Danielle Albuquerque Pires

doi:10.5123/s2176-62232015000400003

Rev Pan-Amaz Saude

2015

DOI: 10.5123/s2176-62232015000400003

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Use of different Taq DNA polymerases for detection of Chlamydia trachomatis in cervical samples

Francisca Laís Araújo de Oliveira

M. J. Azevedo

Anderson Nogueira Barbosa

et al.

Abstract: Use of different Taq DNA polymerases for detection of Chlamydia trachomatis in cervical samples O uso de diferentes Taq DNA polimerases para a detecção de Chlamydia trachomatis em amostras cervicais El uso de diferentes Taq DNA polimerasas para la detección de Chlamydia trachomatis en muestras cervicales

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2018

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Molecular tools for phytoplankton monitoring samples

Frazão

Silva

2018

Preprint

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HABs can have severe impacts in fisheries or human health by the consumption of contaminated bivalves. Monitoring assessment (quantitative and qualitative identification) of these organisms, is routinely accomplished by microscopic identification and counting of these organisms. Nonetheless, molecular biology techniques are gaining relevance, once these approaches can easily identify phytoplankton organisms at species level and even cell number quantifications. This work tests 12 methods/kits for genomic DNA extraction and seven DNA polymerases to determine which is the best method for routinely use in a common molecular laboratory, for phytoplankton monitoring samples analyses. From our work, Direct PCR master mix for tissue samples, proved to be the most adequate by its velocity of processivity, practicability, reproducibility, sensitiveness and robustness. However, brands such as Omega Biotek, GRISP, Qiagen and MP Biomedicals also showed good results for conventional DNA extraction as well as all the Taq brands tested (GRISP, GE Healthcare Life Sciences, ThermoFisher Scientific and Promega). Lugol´s solution, with our tested kits did not show negative interference in DNA amplification. The same can be said about mechanical digestion, with no significant differences among kits with or without this homogenization step.

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Molecular tools for phytoplankton monitoring samples

Frazão

Silva

2018

Preprint

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Use of different Taq DNA polymerases for detection of Chlamydia trachomatis in cervical samples

Cited by 1 publication

References 15 publications

Molecular tools for phytoplankton monitoring samples

Molecular tools for phytoplankton monitoring samples

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