Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum

Corbo, Christopher; Othman, Nidaa A.; Gutkin, Michael; Alonso, Alejandra; Fülöp, Zoltán

doi:10.1002/jemt.21061

Cited by 20 publications

(22 citation statements)

References 22 publications

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“…Zebrafish radial glia express aquaporin-4, although its localization is not polarized in the same way as in mammalian astrocytes (Grupp et al 2010). Zebrafish radial glial endfeet also express markers of tight junctions much like mammalian astrocytic endfeet (Corbo et al 2012). Zebrafish radial glial endfeet also associate with neuronal processes (Marcus and Easter 1995).…”

Section: Fish Radial Glia and Their Relationship To Mammalian Astrocytesmentioning

confidence: 99%

Glial Cell Development and Function in Zebrafish

Lyons

Talbot

2014

Cold Spring Harb Perspect Biol

120

100

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The zebrafish is a premier vertebrate model system that offers many experimental advantages for in vivo imaging and genetic studies. This review provides an overview of glial cell types in the central and peripheral nervous system of zebrafish. We highlight some recent work that exploited the strengths of the zebrafish system to increase the understanding of the role of Gpr126 in Schwann cell myelination and illuminate the mechanisms controlling oligodendrocyte development and myelination. We also summarize similarities and differences between zebrafish radial glia and mammalian astrocytes and consider the possibility that their distinct characteristics may represent extremes in a continuum of cell identity. Finally, we focus on the emergence of zebrafish as a model for elucidating the development and function of microglia. These recent studies have highlighted the power of the zebrafish system for analyzing important aspects of glial development and function.

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Section: Fish Radial Glia and Their Relationship To Mammalian Astrocytesmentioning

confidence: 99%

Glial Cell Development and Function in Zebrafish

Lyons

Talbot

2014

Cold Spring Harb Perspect Biol

120

100

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“…We have been studying adult neurogenesis in the optic tectum, which is a visuomotor center and has six layers (Corbo, Othman, Gutkin, Alonso, & Fulop, ). Most neurons in the optic tectum are located in the periventricular grey zone (PGZ) and extend their apical dendrites into the dorsal layer of the optic tectum to make synapses with axons of the retinal ganglion cells.…”

Section: Introductionmentioning

confidence: 99%

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

Shimizu

Ueda

Ohshima

2018

Glia

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Zebrafish have superior abilities to generate new neurons in the adult brain and to regenerate brain tissue after brain injury compared with mammals. There exist two types of neural stem cells (NSCs): neuroepithelial-like stem cells (NE) and radial glia (RG) in the optic tectum. We established an optic tectum stab injury model to analyze the function of NSCs in the regenerative condition and confirmed that the injury induced the proliferation of RG, but not NE and that the proliferated RG differentiated into new neurons after the injury. We then analyzed the involvement of Wnt signaling after the injury, using a Wnt reporter line in which canonical Wnt signaling activation induced GFP expression and confirmed that GFP expression was induced specifically in RG after the injury. We also analyzed the expression level of genes related to Wnt signaling, and confirmed that endogenous Wnt antagonist dkk1b expression was significantly decreased after the injury. We observed that Wnt signal inhibitor IWR1 treatment suppressed the proliferation and differentiation of RG after the injury, suggesting that up-regulation of Wnt signaling in RG after the stab injury was required for optic tectum regeneration. We also confirmed that Wnt activation by treatment with GSK3β inhibitor BIO in uninjured zebrafish induced proliferation of RG in the optic tectum. This optic tectum stab injury model is useful for the study of the molecular mechanisms of brain regeneration and analysis of the RG functions in physiological and regenerative conditions.

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“…This domain, homologous to the superior colliculus of mammals, is composed of a deep granular layer of periventricular interneurons, projection neurons (periventricular grey zone, PGZ) and ventricular RG cells, and a superficial neuropile layer receiving input from retinal ganglion cells (Corbo et al, 2012). One key feature of this structure is to continuously expand from a growth zone located at its posterior edge (tectal proliferation zone, TPZ) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain

Galant

Furlan

Coolen

et al. 2016

Developmental Biology

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Neurogenesis in the post-embryonic vertebrate brain varies in extent and efficiency between species and brain territories. Distinct neurogenesis modes may account for this diversity, and several neural progenitor subtypes, radial glial cells (RG) and neuroepithelial progenitors (NE), have been identified in the adult zebrafish brain. The neurogenic sequences issued from these progenitors, and their contribution to brain construction, remain incompletely understood. Here we use genetic tracing techniques based on conditional Cre recombination and Tet-On neuronal birthdating to unravel the neurogenic sequence operating from NE progenitors in the zebrafish post-embryonic optic tectum. We reveal that a subpopulation of her5-positive NE cells of the posterior midbrain layer stands at the top of a neurogenic hierarchy involving, in order, the amplification pool of the tectal proliferation zone (TPZ), followed by her4-positive RG cells with transient neurogenic activity. We further demonstrate that the adult her5-positive NE pool is issued in lineage from an identically located NE pool expressing the same gene in the embryonic neural tube. Finally, we show that these features are reminiscent of the neurogenic sequence and embryonic origin of the her9-positive progenitor NE pool involved in the construction of the lateral pallium at post-embryonic stages. Together, our results highlight the shared recruitment of an identical neurogenic strategy by two remote brain territories, where long-lasting NE pools serve both as a growth zone and as the life-long source of young neurogenic RG cells.

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Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum

Cited by 20 publications

References 22 publications

Glial Cell Development and Function in Zebrafish

Glial Cell Development and Function in Zebrafish

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain

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