Use of Dielectric Heating (Shortwave Diathermy) in Thawing Frozen Suspensions of Tissue Culture Cells.

Breast Cancer Cell Line Development and Authentication

“…At the peak of production, 20,000 cultures were shipped per week and a total of 600,000 cultures were shipped in the two years the lab was in existence (Brown and Henderson, 1983). This, along with the Lewis Coriell's development of the laminar flow hood to reduce contamination of cell cultures and methods to freeze and recover cell lines (Coriell et al, 1958;Coriell, 1973, 1974) (Coriell and McGarrity, 1968;Greene et al, 1964;McAllister and Coriell, 1956;Silver et al, 1964), led to the establishment of cell repositories to house and distribute cells. It also led to the development of tumor specific cancer cell lines that created models of different types of human cancer and to an explosion of understanding of how cells work without the influence or perturbation of other cells.…”