Use of Diagnostic SSR Markers for Identification of Larix Lyallii and L. Occidentalis (Pinaceae)

Nadeem, Shaheer; Jaquish, Barry; Newton, Craig; Khasa, P. D.

doi:10.1017/s0960428603000052

Cited by 5 publications

(7 citation statements)

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“…Semerikov & Lascoux 1999;Gros-Louis et al 2005), no signs of natural hybridization were detected in any of the 840 individuals sampled. It must be noted that two of the primers pairs used in the present study (UAKLla1 and UAKLla2) have previously revealed species-specific alleles and could be used to track for the presence of hybrids or introgressants (Nadeem et al 2003). The mean fixation index per population (f) was lower in subalpine larch (0.075) than in western larch (0.103; t-test; P < 0.05).…”

Section: Numerical Analysismentioning

confidence: 69%

“…2005), no signs of natural hybridization were detected in any of the 840 individuals sampled. It must be noted that two of the primers pairs used in the present study ( UAKLla1 and UAKLla2 ) have previously revealed species‐specific alleles and could be used to track for the presence of hybrids or introgressants (Nadeem et al . 2003).…”

Section: Resultsmentioning

confidence: 99%

“…2001). In one hand, the amount of interspecific gene flow since the establishment of the analysed stands should be negligible as no natural hybridization was detected with the diagnostic markers ( UAKLla1 and UAKLla2 ) previously developed for these species (Nadeem et al . 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Ledig et al 2001;Molina-Freaner et al 2001). In one hand, the amount of interspecific gene flow since the establishment of the analysed stands should be negligible as no natural hybridization was detected with the diagnostic markers (UAKLla1 and UAKLla2) previously developed for these species (Nadeem et al 2003). However, it must be noted that some putative hybrids have been previously reported in the narrow zone of contact between subalpine larch and western larch in Montana (Carlson 1995).…”

Section: Contrasting Genetic Variation and Contrasting Demographic Anmentioning

confidence: 92%

“…All these markers were transferable and polymorphic in L. occidentalis (see Khasa et al . 2000 for more details), and two of them ( UAKLLa1 and UAKLLa2 ) harboured species‐specific variants between western and subalpine larches (Nadeem et al . 2003).…”

Section: Methodsmentioning

confidence: 99%

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Contrasting microsatellite variation between subalpine and western larch, two closely related species with different distribution patterns

et al. 2006

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Subalpine larch (Larix lyallii Parl.) and western larch (Larix occidentalis Nutt.) represent two closely related species with contrasting abundance and distribution patterns in Western North America. Genetic diversity at seven informative microsatellite loci was determined for 19 populations of subalpine larch and nine populations of western larch. Contrasting genetic diversity and patterns of population differentiation were observed between the two species. The overall within-population genetic diversity parameters were lower in subalpine larch (A = 3.2; A(P) = 3.6; H(E) = 0.418) than in western larch (A(P) = 5.51; H(E) = 0.580), a pattern that is likely related to historical or demographic factors. No evidence of interspecific hybridization was observed. Significantly more population differentiation (theta = 0.15; R(ST) = 0.07), consistent with more restricted gene flow, was observed for subalpine larch as compared to western larch (theta = 0.05; R(ST) = 0.04). Under the assumption of an infinite allele mutation model, 12 of the 19 subalpine larch populations showed signs of deviation from the mutation-drift equilibrium, which suggests Holocene population bottlenecks and fluctuations in effective population size for this species. None of the western larch populations deviated significantly from the mutation-drift equilibrium. For both species, Mantel's test revealed a significant positive relationship between geographical and genetic distances indicative of isolation by distance. A similar geographical structure was detected in both species, suggesting at least two genetically distinct glacial populations in each species. The various implications for gene conservation are discussed.

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Section: Numerical Analysismentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Contrasting Genetic Variation and Contrasting Demographic Anmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Contrasting microsatellite variation between subalpine and western larch, two closely related species with different distribution patterns

et al. 2006

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Recolonization and radiation in Larix (Pinaceae): evidence from nuclear ribosomal DNA paralogues

Wei

Wang

2004

Molecular Ecology

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Gene paralogy frequently causes the conflict between gene tree and species tree, but sometimes the coexistence of a few paralogous copies could provide more markers for tracing the phylogeographical process of some organisms. In the present study, nrDNA ITS paralogues were cloned from all but one species of Larix, an Eocene genus having two sections, Larix and Multiserialis, with a huge circumboreal distribution and an Eastern Asia-Western North America disjunction, respectively. A total of 96 distinct clones, excluding five putative pseudogenes or recombinants, were obtained and used in the gene genealogy analysis. The clones from all Eurasian species of section Larix are mixed together, suggesting that recolonization and recent morphological differentiation could have played important roles in the evolution of this section. In contrast, the species diversification of the Eurasian section Multiserialis may result from radiation in the east Himalayas and its vicinity, considering extensive nrDNA founder effects in this group. Our study also suggests that the distribution pattern analysis of members of multiple gene family would be very useful in tracking the evolutionary history of some taxa with recent origin or rapid radiation that cannot be resolved by other molecular markers.

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Genetic diversity, population structure, and differentiation of Siberian larch, Gmelin larch, and Cajander larch on SSR-marker data

2013

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Use of Diagnostic SSR Markers for Identification of Larix Lyallii and L. Occidentalis (Pinaceae)

Cited by 5 publications

References 0 publications

Contrasting microsatellite variation between subalpine and western larch, two closely related species with different distribution patterns

Contrasting microsatellite variation between subalpine and western larch, two closely related species with different distribution patterns

Recolonization and radiation in Larix (Pinaceae): evidence from nuclear ribosomal DNA paralogues

Genetic diversity, population structure, and differentiation of Siberian larch, Gmelin larch, and Cajander larch on SSR-marker data

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