Use of detergents in two-dimensional crystallization of membrane proteins

Rigaud, Jean‐Louis; Chami, Mohammed Amine; Lambert, Olivier; Lévy, Daniel; Ranck, Jean-Luc

doi:10.1016/s0005-2736(00)00307-2

Cited by 86 publications

(66 citation statements)

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“…In addition to the composition of the solution (salt, pH, additives such as glycerol) and temperature, variables of primary importance are related to protein-protein, lipid-protein, detergent-protein and detergent-lipid interactions. As opposed to proteoliposome reconstitution, the rules for 2-D crystallization by detergent removal are far from being generalized and very few physicochemical parameters have been determined as essential for growing 2-D crystals of membrane proteins (10,44,45).…”

Section: -D Crystallization By Detergent Removal In Bulk Solutionmentioning

confidence: 99%

“…Second, structure determination at high resolution is actually a difficult challenge for membrane proteins and the number of membrane proteins that have been crystallized in three dimensions (3-D) is still small and far behind that of soluble proteins (6). Reconstitution of membrane proteins into artificial membranes to form 2-D crystals has opened a new way to solve their structures inside a native-like environment (7)(8)(9)(10). Indeed, electron crystallography of 2-D crystals has developed to the point that significant structural atomic models have been built and that many membrane protein structures have been evaluated at resolutions allowing the secondary structure to be assessed (11).…”

Section: Introductionmentioning

confidence: 99%

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Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals

2002

Braz J Med Biol Res

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Reconstitution of membrane proteins into lipid bilayers is a powerful tool to analyze functional as well as structural areas of membrane protein research. First, the proper incorporation of a purified membrane protein into closed lipid vesicles, to produce proteoliposomes, allows the investigation of transport and/or catalytic properties of any membrane protein without interference by other membrane components. Second, the incorporation of a large amount of membrane proteins into lipid bilayers to grow crystals confined to two dimensions has recently opened a new way to solve their structure at high resolution using electron crystallography. However, reconstitution of membrane proteins into functional proteoliposomes or 2-D crystallization has been an empirical domain, which has been viewed for a long time more like "black magic" than science. Nevertheless, in the last ten years, important progress has been made in acquiring knowledge of lipid-protein-detergent interactions and has permitted to build upon a set of basic principles that has limited the empirical approach of reconstitution experiments. Reconstitution strategies have been improved and new strategies have been developed, facilitating the success rate of proteoliposome formation and 2-D crystallization. This review deals with the various strategies available to obtain proteoliposomes and 2-D crystals from detergent-solubilized proteins. It gives an overview of the methods that have been applied, which may be of help for reconstituting more proteins into lipid bilayers in a form suitable for functional studies at the molecular level and for high-resolution structural analysis. Correspondence

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Section: -D Crystallization By Detergent Removal In Bulk Solutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals

2002

Braz J Med Biol Res

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“…Finding the best detergent for any given membrane protein is a matter of trial and error. Nevertheless, many membrane proteins can be solubilized and purified using a small collection of detergents [28]. The purification of solubilized membrane proteins uses similar techniques to those employed for soluble proteins.…”

Section: Membrane Protein Production and Purificationmentioning

confidence: 99%

“…Kaufmann et al (2005) have recently presented a device that allows exact determination of detergent concentrations during purification and crystallization trials using minimal sample quantities [34]. Here we briefly list and summarize the key aspect of the different approaches that have been successfully used in the past to generate 2D-crystals [28,[35][36][37][38].…”

Section: Two-dimensional Membrane Protein Crystallizationmentioning

confidence: 99%

Milestones in electron crystallography

Renault

Chou

Chiu

et al. 2006

J Comput Aided Mol Des

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SummaryElectron crystallography determines the structure of membrane embedded proteins in the twodimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for the imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

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“…Carbohydrate surfactants are of great interest because they are not noxious for the environment. 12,13 Due to their functional properties they can be used in several areas, such as food industry (emulsion stabilization, foaming), 14,15 biology (extraction membrane proteins), 16 glycobiology, 17,18 immunology, 19 detergents, and cosmetology (non-alergic compounds).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and antimicrobial activity of amphiphilic carbohydrate derivatives

Reis¹,

Oda²,

Almeida³

et al. 2008

J. Braz. Chem. Soc.

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Diaminas N-monoalquiladas foram sintetizadas e tratadas com D-ribonolactona ou D-gliconolactona. As aldonamidas resultantes foram avaliadas para atividade antimicrobiana contra S. aureus, E. coli, M. tuberculosis and C. albicans. Duas hidrazidas foram também preparadas a partir da ribonoidrazida e suas atividades biológicas comparadas com aquelas de análogos amida. Todos os derivados da ribonolactona mostraram ter atividade antitubercular moderada, alguns foram ativos também contra S. aureus. N-monoalkylated diamines were synthesised and treated with D-ribonolactone or D-gluconolactone. The resulting aldonamides were evaluated for their antimicrobial activity against S. aureus, E. coli, M. tuberculosis and C. albicans. Two hydrazides were also prepared from ribonohydrazide and their biological activity was compared to their amide analogues. All the ribono-derivatives displayed moderated antitubercular activity, and some of them were also active against S. aureus. Keywords: antimicrobial, M. tuberculosis, amphiphilic, aldonamide IntroductionSurfactants are amphiphilic molecules which are widely used in many industries. Although conventional nonionic surfactants can be produced in large scale from petrochemical raw materials, the increasing need for biodegradable and less toxic products has led to numerous studies of new sugar-based surfactants, which can be prepared from natural raw materials. [1][2][3][4][5][6][7][8][9][10][11] These compounds possess a carbohydrate hydrophilic head (mono-or oligosaccharide), and a hydrophobic tail, usually derived from a fatty acid. The two moieties can be directly linked via a functional group (ester, ether, hydrazine, amine, etc) or separated by a spacer (gemini surfactants). Carbohydrate surfactants are of great interest because they are not noxious for the environment. 12,13 Due to their functional properties they can be used in several areas, such as food industry (emulsion stabilization, foaming), 14,15 biology (extraction membrane proteins), 16 glycobiology, 17,18 immunology, 19 detergents, and cosmetology (non-alergic compounds).Carbohydrate-derivated amphiphilic compounds have also been studied for their antimicrobial action. [20][21][22][23][24] These compounds can display two mechanisms of action. They can act as nonionic surfactants: the hydrophilic moiety of surface active compounds binds to the hydrophilic portion of the membrane via hydrogen bonds. The hydrophobic moiety is then able to penetrate the lipid bilayer structure, provocating a disorder in the permeability and fluidity of the membrane. 25 They can also act as inhibitors of enzymes involved in the biosynthesis of the bacterial cell wall. Galactofuranosyl 1 26 and galactopyranosyl derivatives 2 27 and 3 28 (Figure 1) bearing long alkyl chains displayed antitubercular activity.In the last years the intensive use of antibiotic has lead to an increase of the emergence of resistant bacteria. 29,30 There is a growing need for new class of antibacterial compounds having different mechanism of action compar...

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Use of detergents in two-dimensional crystallization of membrane proteins

Cited by 86 publications

References 100 publications

Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals

Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals

Milestones in electron crystallography

Synthesis and antimicrobial activity of amphiphilic carbohydrate derivatives

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