Use of Deep-Freezing in the Preservation and Preparation of Fresh Soil Samples

Effects of Freezing and Thawing on Some Chemical Properties of Three Soils

“…Gasser (4) reported that freezing soil at -10 c did not affect the amount of mineral-I,l produced on subsequent incubation, but that frequent freezing and thawing over the same period appeared to increase the amount produced.…”

Winter Changes in Soil Nitrate and Exchangeable Ammonium

“…The soil temperature at 1 cm under a grass sward on the meteorological site adjacent to the site at which the cylinder experiment was located was at or below 0 C from Nov. 5,1966 Five days after emplacement of the cylinders the slope of the soil temperature profile (Fig. 3, curve 1 (Fig.…”

Paired prairie and cultivated soils: soluble organics and nitrogen dynamics

“…Most studies concerning the effect of freezing on N mineralization have concentrated on 10 day or longer incubations and none of the studies has evaluated short term mineralization rates (Gasser, 1956;Soulides and Allison, 1961;Ross et al, 1980). Though this work is valid for the use of "ultra-low" temperature freezer or liquid N2 storage, the extremely low temperature bears little resemblance to natural conditions.…”

“…Literature is mixed, however, regarding the effect of freezing and thawing on mineralization of soil N (Gasser, 1956;Soulides and Allison, 1961;Mack, 1963;Campbell et al, 1971;Ross et al 1980). There has been no standardized approach for studying the effect of freezing and thawing soils on N mineralization nor have the differences in soil properties been addressed with regard to this subject.…”