Use of Cord Blood Cells for Banking and Transplant

Gluckman, Éliane; Rocha, Vanderson; Chastang, Claude

doi:10.1634/theoncologist.2-5-340

Cited by 12 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While conditioning consisting of busulfan, melphalan, and ATG has been demonstrated to be well-tolerated among very young UCBT recipients, unusually delayed engraftment and a high incidence of graft failure were observed [13]. Day 100 TRM of 15% to 50% has been observed in MAC UCBT recipients, depending on the underlying disease and disease status before transplantation [7][8][9]11,12,14,29,36,42,47,[69][70][71][72]. Significant causes of day-100 TRM include infection, organ failure, acute GVHD, and hepatic veno-occlusive disease.…”

Section: Conditioning Regimensmentioning

confidence: 99%

Cord blood transplantation and stem cell regenerative potential

Liao

Geyer

Yang

et al. 2011

Experimental Hematology

View full text Add to dashboard Cite

Section: Conditioning Regimensmentioning

confidence: 99%

Cord blood transplantation and stem cell regenerative potential

Liao

Geyer

Yang

et al. 2011

Experimental Hematology

View full text Add to dashboard Cite

“…In our previous study, we showed that hUCB-MSCs express OCT4A and REX1, a well-known transcription factors that are characteristic markers of pluripotent stem cells, and REX1 expression in hUCB-MSCs was nearly five-fold greater than in hBM-MSCs [12] , [13] . Moreover, hUCB-MSCs provide no ethical barriers for basic studies and clinical applications [14] , [15] .…”

Section: Introductionmentioning

confidence: 99%

Implication of NOD1 and NOD2 for the Differentiation of Multipotent Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

et al. 2010

View full text Add to dashboard Cite

Toll-like receptors (TLRs) and Nod-like receptors (NLRs) are known to trigger an innate immune response against microbial infection. Although studies suggest that activation of TLRs modulate the function of mesenchymal stem cells (MSCs), little is known about the role of NLRs on the MSC function. In this study, we investigated whether NOD1 and NOD2 regulate the functions of human umbilical cord blood-derived MSCs (hUCB-MSCs). The genes of TLR2, TLR4, NOD1, and NOD2 were expressed in hUCB-MSCs. Stimulation with each agonist (Pam3CSK4 for TLR2, LPS for TLR4, Tri-DAP for NOD1, and MDP for NOD2) led to IL-8 production in hUCB-MSC, suggesting the expressed receptors are functional in hUCB-MSC. CCK-8 assay revealed that none of agonist influenced proliferation of hUCB-MSCs. We next examined whether TLR and NLR agonists affect osteogenic-, adipogenic-, and chondrogenic differentiation of hUCB-MSCs. Pam3CSK4 and Tri-DAP strongly enhanced osteogenic differentiation and ERK phosphorylation in hUCB-MSCs, and LPS and MDP also slightly did. Treatment of U0126 (MEK1/2 inhibitor) restored osteogenic differentiation enhanced by Pam3CSK4. Tri-DAP and MDP inhibited adipogenic differentiation of hUCB-MSCs, but Pam3CSK4 and LPS did not. On chondrogenic differentiation, all TLR and NLR agonists could promote chondrogenesis of hUCB-MSCs with difference in the ability. Our findings suggest that NOD1 and NOD2 as well as TLRs are involved in regulating the differentiation of MSCs.

show abstract

“…Recent reports indicate that infusion of hematopoietic stem/progenitor cells is an important therapeutic approach for the treatment of hematological malignant and ischemic diseases [1] , [2] , [3] , [4] , [5] , [6] . However, insufficient number of donor stem cells, cell viability and inefficient expansion techniques limit its clinical application [1] , [2] , [3] , [4] . Therefore, an efficient and practical ex vivo stem cell expansion methodology is critical, which would maintain the expanded stem cell's potential for engraftment, differentiation and long-term sustainability [7] .…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Therapy with Overexpressed VEGF and PDGF Genes Improves Cardiac Function in a Rat Infarct Model

et al. 2009

View full text Add to dashboard Cite

BackgroundTherapeutic potential was evaluated in a rat model of myocardial infarction using nanofiber-expanded human cord blood derived hematopoietic stem cells (CD133+/CD34+) genetically modified with VEGF plus PDGF genes (VIP).Methods and FindingsMyocardial function was monitored every two weeks up to six weeks after therapy. Echocardiography revealed time dependent improvement of left ventricular function evaluated by M-mode, fractional shortening, anterior wall tissue velocity, wall motion score index, strain and strain rate in animals treated with VEGF plus PDGF overexpressed stem cells (VIP) compared to nanofiber expanded cells (Exp), freshly isolated cells (FCB) or media control (Media). Improvement observed was as follows: VIP>Exp> FCB>media. Similar trend was noticed in the exercise capacity of rats on a treadmill. These findings correlated with significantly increased neovascularization in ischemic tissue and markedly reduced infarct area in animals in the VIP group. Stem cells in addition to their usual homing sites such as lung, spleen, bone marrow and liver, also migrated to sites of myocardial ischemia. The improvement of cardiac function correlated with expression of heart tissue connexin 43, a gap junctional protein, and heart tissue angiogenesis related protein molecules like VEGF, pNOS3, NOS2 and GSK3. There was no evidence of upregulation in the molecules of oncogenic potential in genetically modified or other stem cell therapy groups.ConclusionRegenerative therapy using nanofiber-expanded hematopoietic stem cells with overexpression of VEGF and PDGF has a favorable impact on the improvement of rat myocardial function accompanied by upregulation of tissue connexin 43 and pro-angiogenic molecules after infarction.

show abstract

Use of Cord Blood Cells for Banking and Transplant

Cited by 12 publications

References 13 publications

Cord blood transplantation and stem cell regenerative potential

Cord blood transplantation and stem cell regenerative potential

Implication of NOD1 and NOD2 for the Differentiation of Multipotent Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

Stem Cell Therapy with Overexpressed VEGF and PDGF Genes Improves Cardiac Function in a Rat Infarct Model

Contact Info

Product

Resources

About