Use of convertible flow cells to simulate the impacts of anthropogenic activities on river biofilm bacterial communities

Liao, Kailingli; Bai, Yaohui; Huo, Yang; Jian, Zhiyu; Hu, Wanchao; Zhao, Chen; Qu, Jiuhui

doi:10.1016/j.scitotenv.2018.10.363

Cited by 23 publications

(13 citation statements)

References 43 publications

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“…The corresponding equity of the points 1 and 2 was 0.646934461 and 0.77048394, respectively. These data support an increase in the microbial load in the region with greater distribution of substrate consumption in point two, leading to an increased diversity index (H) [28,30].…”

Section: Resultssupporting

confidence: 67%

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Neto¹,

Araújo²,

Silva³

et al. 2019

JPP

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Mangroves are environments that have fast cycles associated with high concentration of bacterial decomposers. These are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance genes. This study aims to evaluate the metabolic profile of the microbiota in mangrove sediments, and verify the presence of Gram-negative bacteria resistant to meropenem. The samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing concentrations. The DNA was analyzed by multiplex PCR for detecting resistance genes for β-lactam antibiotics. The bacteria were identified by Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The genes were sequenced by ABI PRISM 3100, analyzed by MEGA 6.0 program, and the sequence identified was assessed by GenBank using the BLAST algorithm. Ecoplate® the kit was used to determine the metabolic profile of the microbiota. The results of the six bacterial isolates showed the bla KPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas putida. The samples showed a greater diversity Shannon index, a rich substrate consumption and high equity. There was a metabolic richness such as carbon consumption profiles, being a factor of adaptation of pathogenic bacteria carrying resistance genes to β-lactamics antibiotics.

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Section: Resultssupporting

confidence: 67%

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Neto¹,

Araújo²,

Silva³

et al. 2019

JPP

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“…Next, the suspension was added into the wells (150 µL) of the BIOLOG ECO microplate, and incubated at 25 • C for 7 days [50]. During the incubation, the absorbance value was determined at 590 every 12 h using a multifunctional enzyme label tester (Bio Tek, Winooski, VT, USA) for 7 to 10 days, and based on previous studies [51][52][53][54].…”

Section: Biolog Eco Microplate Test Methodsmentioning

confidence: 99%

Microbial Carbon Metabolic Functions in Sediments Influenced by Resuspension Event

Zhang

Ding

et al. 2020

Water

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Microorganisms in sediments are an important part of the aquatic ecosystem, and their functional activities are sensitive to external environmental pressure. Shallow lakes are characterized by frequent sediment resuspension events, leading to large amounts of nutrients being released. However, information about the potential impacts of sediment resuspension events on the functional activities of microbial communities is limited. In this study, the responses of microbial carbon metabolism in sediments under different wind–wave disturbance were analyzed by BIOLOG ECO microplates. The results showed that under four disturbance conditions (wind speeds of 0, 1.60, 3.62, and 14.10 m/s), the total carbon metabolism function of the sediment microbial community (represented as average well-color development, AWCD) remained unchanged (p > 0.05), and the final total AWCD value stabilized at about 1.70. However, compared with the control group, some specific carbon sources (e.g., amines and carboxylic acids) showed significant changes (p < 0.05). We found that short-term (8 h) resuspension events did not affect the total carbon metabolism of sediment microbial communities, while it affected the microbial utilization ability of some specific types of carbon sources. For example, we found that the microbial utilization capacity of polymers in the 14.10 m/s group was the best. This study provides a new insight into the carbon cycle process of shallow lake sediments that resuspension events will affect the carbon cycle process of sediments.

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“…The flow cell system was kept free of air bubbles by a bubble trap, which creates low positive pressure on the medium flow, thus mitigating undesirable peristaltic pulsation in liquid delivery to the flow cells. According to the sluggish flow of the Chaobai River 2 , the flow rate in our culturing device was set at a constant rate of 57.33 μl/min using a multichannel peristaltic pump (BT100-1L, Longer Precision Pump, Co., Ltd., Baoding, China) to create favorable conditions for biofilm culturing (Liao et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Fungal Community as a Bioindicator to Reflect Anthropogenic Activities in a River Ecosystem

et al. 2018

Self Cite

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The fungal community interacts with the ambient environment and can be used as a bioindicator to reflect anthropogenic activities in aquatic ecosystems. Several studies have investigated the impact of anthropogenic activities on the fungal community and found that community diversity and composition are influenced by such activities. Here we combined chemical analysis of water properties and sequencing of fungal internal transcribed spacer regions to explore the relationship between water quality indices and fungal community diversity and composition in three river ecosystem areas along a gradient of anthropogenic disturbance (i.e., less-disturbed mountainous area, wastewater-discharge urban area, and pesticide and fertilizer used agricultural area). Results revealed that the level of anthropogenic activity was strongly correlated to water quality and mycoplankton community. The increase in organic carbon and nitrogen concentrations in water improved the relative abundance of Schizosaccharomyces, which could be used as a potential biomarker to reflect pollutant and nutrient discharge. We further applied a biofilm reactor using water from the three areas as influent to investigate the differences in fungal communities in the formed biofilms. Different community compositions were observed among the three areas, with the dominant fungal phyla in the biofilms found to be more sensitive to seasonal effects than those found in water. Finally, we determined whether the fungal community could recover following water quality restoration. Our biofilm reactor assay revealed that the recovery of fungal community would occur but need a long period of time. Thus, this study highlights the importance of preserving the original natural aquatic ecosystem.

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Use of convertible flow cells to simulate the impacts of anthropogenic activities on river biofilm bacterial communities

Cited by 23 publications

References 43 publications

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Microbial Carbon Metabolic Functions in Sediments Influenced by Resuspension Event

Fungal Community as a Bioindicator to Reflect Anthropogenic Activities in a River Ecosystem

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