Use of complex supplements and light-differential effects for micropropagation of Hadrolaelia purpurata (= Laelia purpurata) and Encyclia randii orchids

Gonçalves, Letícia de Menezes; Prizão, Eliane Cristina; Milaneze-Gutierre, Maria Auxiliadora; Mangolin, Claudete Aparecida; Machado, Maria de Fátima Pires da Silva

doi:10.4025/actasciagron.v34i4.12333

Cited by 7 publications

(5 citation statements)

References 12 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in agreement with Arditti (1992) who proposed that for in vitro growth of PLBs and seedling of an orchid, some complex additives were satisfactory, while some were unsatisfactory and even inhibitory. Goncalves et al (2012) found that the highest number of seedlings of Laelia purpurata orchid occurred in Knudson C (KC) medium containing 90 g L -1 banana pulp, while the seedling number of Encyclia orchid was high in KC medium containing coconut water. Other research by Nambiar et al (2012) investigated that the best organic additive for proliferation of PLBs of Dendrobium Alya Pink was coconut water compared to banana and tomato homogenate.…”

Section: Resultsmentioning

confidence: 99%

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

Dwiyani¹,

Yuswanti²,

Darmawati³

et al. 2015

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

Dwiyani¹,

Yuswanti²,

Darmawati³

et al. 2015

Agrivita.J.Agr.Sci

View full text Add to dashboard Cite

show abstract

“…Among the various organic supplements studied (CW, PH, and BH), BH at a concentration of 25 or 50 g•L −1 improved shoot and root number and shoot and root length [48]. Gonçalves et al [49] found that the number of Hadrolaelia purpurata seedlings produced was the highest on KC medium containing 90 g•L −1 of BH. Furthermore, Utami and Hariyanto [50] observed the effect of supplementation with 150 mL•L −1 CW with different BH concentrations (0, 5, 10, 15 g•L −1 ) on the plantlet development of Phalaenopsis amboinensis and found that the organic supplementation of CW together with BH had significantly increased the growth of plantlets.…”

Section: Banana Homogenatementioning

confidence: 99%

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Utami

Hariyanto

2020

International Journal of Agronomy

View full text Add to dashboard Cite

In nature, orchid seed germination is obligatory following infection by mycorrhizal fungi, which supplies the developing embryo with water, carbohydrates, vitamins, and minerals, causing the seeds to germinate relatively slowly and at a low germination rate. The nonsymbiotic germination of orchid seeds found in 1922 is applicable to in vitro propagation. The success of seed germination in vitro is influenced by supplementation with organic compounds. Here, we review the scientific literature in terms of the contents and role of organic supplements in promoting seed germination, protocorm development, and seedling growth in orchids. We systematically collected information from scientific literature databases including Scopus, Google Scholar, and ProQuest, as well as published books and conference proceedings. Various organic compounds, i.e., coconut water (CW), peptone (P), banana homogenate (BH), potato homogenate (PH), chitosan (CHT), tomato juice (TJ), and yeast extract (YE), can promote seed germination and growth and development of various orchids. They also stimulate seedling development, formation of protocorm-like bodies (PLBs), plantlet growth, and multiple shoot formation. The addition of organic compounds to culture media, individually or in combination, accelerates seed germination and seedling development. Different types and concentrations of organic nutrients are needed for the success of in vitro cultures, depending on the species and genotype.

show abstract

“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

View full text Add to dashboard Cite

Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

show abstract

Use of complex supplements and light-differential effects for micropropagation of Hadrolaelia purpurata (= Laelia purpurata) and Encyclia randii orchids

Cited by 7 publications

References 12 publications

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

IN VITRO GERMINATION AND ITS SUBSEQUENT GROWTH OF AN ORCHID OF Vanda tricolor Lindl. var. suavis FROM BALI ON COMPLEX ADDITIVES ENRICHED MEDIUM

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Contact Info

Product

Resources

About