Therapeutic phlebotomy also known as venesection or bloodletting is the therapeutic removal of blood from the body that has been practiced since ancient times to treat illnesses [1]. It began in Egypt around 3000 years ago then spread around the world [2, 3]. Its use gradually declined in the late 19th century and it was almost abandoned during the 20th century [4, 5]. In the past, bloodletting was practiced using lancets, cupping, and the application of leeches for localized bleeding to virtually treat illnesses [6, 5]. At present day, however, therapeutic phlebotomy is being used in modern medicine as an essential part of the treatment for various disorders including hemochromatosis, polycythemia vera and porphyria cutanea tarda [7]. Despite significant advances in conventional medicine, there is a high prevalence and increased public interest Background and objectives: Therapeutic phlebotomy is the removal of blood from the body that has been practiced since ancient time for treating illnesses. Recently, interest in wet-cupping to treat various hematological disorders particularly polycythemia in public has grown. The present study aims to evaluate the effectiveness of wet-cupping versus blood donation on hematological parameters. Methods: In this pre and post-test experimental study, a total of 60 healthy young adults were purposively assigned non-randomly either in cupping (n=30) or blood donation (n=30) groups. Blood samples were collected from all subjects to measure hematological parameters before and one week after interventions. Subjects of wet-cupping and blood donation were matched in age and baseline hematological parameters. The hematological parameters were analyzed using hematology analyzer (Symex, Japan). Results: The findings of the study showed a significant difference in hematological parameters of wet-cupping and blood donation including hemoglobin level (Hb) (14.87 g/dl vs. 14.39 g/dl), packed cell volume (PCV) (44.78 % vs. 42.91 %), and red blood cell (RBC) (5.27 1012/uL vs. 4.94 1012/uL), respectively. While the values of other parameters were not different significantly; mean cell volume (MCV) (85.15 fl vs. 86.95 fl), mean corpuscular hemoglobin (MCH) (28.31 pg vs. 29.20 pg), mean corpuscular hemoglobin concentration (MCHC) (33.24 g/dl vs. 33.57 g/dl), red cell distribution width (RDW) (12.53 % vs. 12.40 %), platelet (245.77 109/L vs. 246.27 109/L), and white blood cell (WBC) (7.47 109/ L vs. 7.47 109/L), respectively. Conclusion: The present study concluded that both wet-cupping and blood donation are effective interventions in reducing HB, PCV, and RBC along with increase platelets in blood donation only.