Use of Commercial Sugar, Isabgol and Ordinary Water in Culture Medium for Conservation of Curcuma longa L

Agrawal, Anuradha; Mahalakshmi, C.; Tyagi, Rishi

doi:10.1007/bf03263266

Cited by 7 publications

(1 citation statement)

References 8 publications

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“…Regarding the root number, a detrimental effect of table sugar on the micropropagation of Solanum tuberosum has been reported by Demo (Demo et al 2008). On the other hand, an increase of the number of roots was observed in Curcuma longa when agar was complemented with cassava starch (Buah 2014) or completely substituted by Isubgol (Agrawal et al 2008). In the chestnut and birch experiments, the root system was not affected by the presence of LC agar or table sugar in the culture media, in agreement with the results previously obtained in other species like Nicotiana tabacum (Ozel et al 2008), Curcuma longa (Jain, Babbar 2006) or banana (Agrawal et al 2010), therefore suggesting that the low-cost substitutes provide a valuable resource for the development of micropropagation protocols.…”

Section: Discussionmentioning

confidence: 99%

Reducing costs, improving profits: A low-cost culture media for woody plants micropropagation

Castro-Camba,

Vielba,

Covelo

et al. 2023

J. For. Sci.

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Micropropagation has enormous potential in the forestry industry for the mass production of elite genotypes. A limiting factor that hinders a wider adoption of this technique is its high associated cost, dissuading small producers and laboratories from its use. Thus, it is important to develop low-cost protocols to increase the competitiveness of micropropagation. The goal of the present study was to elaborate a low-cost culture medium that would allow plant growth without diminishing plant quality and production. The replacement of laboratory sucrose with commercial sugar as a carbon source, and laboratory agar with locally produced agar as a gelling agent was tested. To validate the effects of the medium for woody species micropropagation, two relevant forest species, chestnut and birch, were used and several phenotypic characteristics were recorded. Our data indicated that the substitutions made in the media composition did not alter growth or rooting parameters. Substitution of laboratory sucrose and Bacto agar by table sugar and local commercial agar provides a valuable alternative for forest species micropropagation and for increasing their potential profitability.

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