Use of citrate adonitol agar as a selective medium for the isolation of Escherichia fergusonii from a captive reindeer herd

Foster, Geoffrey; Evans, J.; Tryland, Morten; Hollamby, Simon; MacArthur, Isabel; Gordon, E. I.; Harley, Jane; Voigt, K.

doi:10.1016/j.vetmic.2010.01.014

Cited by 15 publications

(18 citation statements)

References 8 publications

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“…fergusonii utilises adonitol for growth and thus generates light yellow-coloured colonies on adonitol-supplemented SCA agar. On the other hand, E. coli typically does not use adonitol or citrate as (Foster et al 2010). However, in this study, E. coli strains were identified that could use adonitol differently.…”

Section: Discussionmentioning

confidence: 67%

“…Current identification methods for E. fergusonii include the use of differential and selective media as well as biochemical profiling methods such as API 20E. The use of citrate adonitol and sorbitol MacConkey media is an important part of the culture process; however, the interpretation of results may be difficult, especially when using specimens with complex bacterial flora such as rectum content (Foster et al 2010;Oh et al 2012). The API 20E identification system is based on 20 different biochemical tests that greatly increase the accuracy of correct identification.…”

Section: Discussionmentioning

confidence: 99%

“…64583101/2016/50). The rectal swab samples were inoculated into 5 ml of tryptic soy broth (Merck Millipore ® , Germany) and incubated at 37 °C for 24 h. Ten microlitres of each culture in TSB were inoculated onto Simmons citrate agar (Oxoid ® , UK) containing 4% adonitol (SCA) as described before (Foster et al 2010) and eosin methylene blue agar for identification of E. coli. The agar plates were incubated at 37 °C for 24 h. Adonitol-positive yellow colonies were transferred to sorbitol MacConkey agar (SMAC) and incubated at 37 °C for 24 h (Wragg et al 2009;Oh et al 2012).…”

Section: Methodsmentioning

confidence: 99%

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Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Parın¹,

Kırkan²,

Arslan³

et al. 2018

Vet. Med.

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ABSTRACT:The aim of this study was to determine the incidence of Escherichia fergusonii in dairy cattle with clinical signs of diarrhoea. The specimens were obtained from three different farms in Denizli province of Turkey, between August 2016 and December 2016. Rectal contents of 57 Holstein-friesian dairy cattle with diarrhoea were collected from farms located in the Aegean Region (Denizli province, Turkey). Rectal swabs were inoculated into enrichment, differential and selective culture media. A total of 49 (86%) Escherichia spp. were isolated by phenotypic identification from 57 rectal swab samples. Presumptive E. fergusonii isolates were tested with the API 20E identification kit and all isolates (100%) were identified as E. coli. Primers targeting specific E. coli and E. fergusonii and genes, including the beta-glucuronidase enzyme, conserved hypothetical cellulose synthase protein and regulator of cellulose synthase and hypothetical protein, putative transcriptional activator for multiple antibiotic resistance were used for detection and differentiation of E. coli and E. fergusonii. Thirteen of the 49 E. coli-verified isolates were identified as E. fergusonii after duplex PCR using EFER 13-and EFER YP-specific primers. Confirmation of strain identity was conducted using Sanger sequencing analysis. The rates of antibiotic resistance of E. fergusonii to penicillin G and erythromycin were 100% and 77%, respectively. In conclusion, field strains of E. fergusonii were detected in cattle with diarrhoea in Turkey, and the strains were found to be resistant to multiple antibiotics.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Parın¹,

Kırkan²,

Arslan³

et al. 2018

Vet. Med.

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“…Since then, E. fergusonii has been recognized as an important emerging opportunistic pathogen of both animals and humans. In animals, E. fergusonii has been isolated from pigs, sheep, cattle, goats, horses, reindeer, ostriches, turkeys, and chickens displaying symptoms of salmonellosis-like infections, including diarrhea as well as mastitis, meningitis, abortion, and septicemia (2)(3)(4)(5)(6). This bacterium also has been isolated from human blood, urine, feces, spinal fluid, and, most often, wound exudates from people with conditions such as sepsis, urinary tract infections, enteric diseases, pancreatic carcinoma, and wound infection (2,3,6,7).…”

mentioning

confidence: 99%

“…Of more than 2,600 poultry farms, over 300 (12%) are located in the province of British Columbia (http://bcchicken.ca/wp-content /uploads/2012/11/BCChickenMarketingBoard-BOOKLETAnnual Report2012.pdf [accessed 4 July 2013]). The majority (over 80%) of British Columbia's poultry farms are located in the Fraser Valley (http://www.chickenfarmers.ca/resources/annual-report-2012/ [accessed 4 July 2013]).…”

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confidence: 99%

Duplex PCR Methods for the Molecular Detection of Escherichia fergusonii Isolates from Broiler Chickens

Simmons

Rempel

Block

et al. 2014

Appl Environ Microbiol

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e Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypothetical protein, putative transcriptional activator for multiple antibiotic resistance), and EFER_3126 (encoding a putative triphosphoribosyl-dephospho-coenzyme A [CoA]), were designed for the detection of E. fergusonii by conventional and real-time PCR methods. Primers were screened by in silico PCR against 489 bacterial genomic sequences and by both PCR methods on 55 reference and field strains. Both methods were specific and sensitive for E. fergusonii, showing amplification only for this bacterium. Conventional PCR required a minimum bacterial concentration of approximately 10 2 CFU/ml, while real-time PCR required a minimum of 0.3 pg of DNA for consistent detection. Standard curves showed an efficiency of 98.5%, with an R 2 value of 0.99 for the real-time PCR assay. Cecal and cloacal contents from 580 chickens were sampled from broiler farms located in the Fraser Valley (British Columbia, Canada). Presumptive E. fergusonii isolates were recovered by enrichment and plating on differential and selective media. Of 301 total presumptive isolates, 140 (46.5%) were identified as E. fergusonii by biochemical profiling with the API 20E system and 268 (89.0%) using PCR methods. E. fergusonii detection directly from cecal and cloacal samples without preenrichment was achieved with both PCR methods. Hence, the PCR methods developed in this work significantly improve the detection of E. fergusonii.

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Investigating the virulence-associated genes and antimicrobial resistance of Escherichia fergusonii Isolated from diseased ostrich chicks

Torkan,

Badouei

2024

Comparative Immunology, Microbiology and Infectious Diseases

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Use of citrate adonitol agar as a selective medium for the isolation of Escherichia fergusonii from a captive reindeer herd

Cited by 15 publications

References 8 publications

Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Molecular identification and antimicrobial resistence of Escherichia fergusonii and Escherichia coli from dairy cattle with diarrhoea

Duplex PCR Methods for the Molecular Detection of Escherichia fergusonii Isolates from Broiler Chickens

Investigating the virulence-associated genes and antimicrobial resistance of Escherichia fergusonii Isolated from diseased ostrich chicks

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