Use of [Cit312,314] filaggrin (306–324) analogue for the diagnosis of rheumatoid arthritis. Conformational study by circular dichroism and fourier transformed infrared spectroscopy

Pérez, Teresa; Gómez, Antoni; Sanmarti, Raimón; Viñas, Odette; Ercilla, Guadalupe; Haro, Isabel

doi:10.1007/bf02447554

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…ELISA Assays. Peptide sequences were coupled covalently to ELISA microplates (Costar Corp., Cambridge, MA, DNA-bind N -oxysuccinimide surface) as previously described. , …”

Section: Methodsmentioning

confidence: 99%

Antibodies to Citrullinated Human Fibrinogen Synthetic Peptides in Diagnosing Rheumatoid Arthritis

et al. 2007

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Since aggressive therapy given early in the rheumatoid arthritis (RA) disease course has the greatest therapeutic potential, early diagnostic tests with both high specificity and sensitivity are desirable. Rheumatoid sera were found to contain antibodies against citrullinated peptides, which are considered to be highly specific markers of RA. In the present work several analogues of the alpha- and beta-chains of fibrin peptides containing different degrees of citrullination have been synthesized and analyzed by ELISA using 111 sera from RA patients. In addition, we have also investigated the synergistic effects of different presentation formats of the synthetic constructs. We have designed chimeric and cyclic peptides that bear different peptide sequences within the same molecule. Our results indicate that the synthesis of peptides bearing fibrinogen and filaggrin domains could be a robust method for the design of useful diagnostic strategies in RA.

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“…ELISA Assays. Peptide sequences were coupled covalently to ELISA microplates (Costar Corp., Cambridge, MA, DNA-bind N -oxysuccinimide surface) as previously described. , …”

Section: Methodsmentioning

confidence: 99%

Antibodies to Citrullinated Human Fibrinogen Synthetic Peptides in Diagnosing Rheumatoid Arthritis

et al. 2007

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“…A filaggrin-derived linear peptide: [Cit 312,314 ]filaggrin(306-324) (filag306) previously synthesized and analysed in our group (20) was included in all ELISA to compare the reactivity and selectivity of fibrin-derived peptides and the filaggrin-derived sequence. Moreover, peptides of the fibrin assayed sequences but containing Arg were used as controls for citrulline to investigate it as an essential constituent of antigenic determinants recognized by RA-specific autoantibodies.…”

Section: Elisamentioning

confidence: 99%

Synthesis of Overlapping Fibrin Citrullinated Peptides and their use for Diagnosing Rheumatoid Arthritis

et al. 2006

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With the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to a-and b-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit 621,627,630 ]a-fibrin(617-631) (afib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.

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“…Following the methodology previously published [37], FTIR spectra were measured with a resolution of 4 cm -1 and 50 scans were taken in order to obtain an appropriate signal to noise ratio without losing signal. Ca 2 F flow cell and a window of 100 µm path length spacer were used.…”

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

Liposome destabilization induced by synthetic lipopeptides corresponding to envelope and non-structural domains of GBV-C/HGV virus. Conformational requirements for leakage

Fernández-Vidal

Rojo

Herrera

et al. 2008

Biophysical Chemistry

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Liposome destabilization induced by synthetic lipopeptides corresponding to envelope and non-structural domains of gbv-c/hgv virus. conformational requirements for leakage, Biophysical Chemistry (2007Chemistry ( ), doi: 10.1016Chemistry ( /j.bpc.2007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT LIPOSOME DESTABILIZATION INDUCED BY SYNTHETIC LIPOPEPTIDES CORRESPONDING TO ENVELOPE AND NON-STRUCTURAL DOMAINS OF GBV-C/HGV VIRUS. CONFORMATIONAL REQUIREMENTS FOR LEAKAGE A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 2 AbstractLiposomes have been used primarily as a model system for studying biological membranes.

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Use of [Cit312,314] filaggrin (306–324) analogue for the diagnosis of rheumatoid arthritis. Conformational study by circular dichroism and fourier transformed infrared spectroscopy

Cited by 7 publications

References 25 publications

Antibodies to Citrullinated Human Fibrinogen Synthetic Peptides in Diagnosing Rheumatoid Arthritis

Antibodies to Citrullinated Human Fibrinogen Synthetic Peptides in Diagnosing Rheumatoid Arthritis

Synthesis of Overlapping Fibrin Citrullinated Peptides and their use for Diagnosing Rheumatoid Arthritis

Liposome destabilization induced by synthetic lipopeptides corresponding to envelope and non-structural domains of GBV-C/HGV virus. Conformational requirements for leakage

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