Use of chitosan and β-tricalcium phosphate, alone and in combination, for bone healing in rabbits

Abstract

The aim of this research was to evaluate the process of bone regeneration in rabbits, using chitosan and beta-tricalcium phosphate (β-TCP) independently and in combination. A total of 12 New Zealand rabbits of both sexes, with average weight of 3.0 ± 0.57 kg were used. Animals were randomly divided into two experimental time points, with six animals euthanized 45 days after surgery and six euthanized 90 days after surgery. We performed two osteotomies in each tibia. The left tibia was used for the chitosan (QUI) and control groups, and the right tibia was used for the β-TCP alone and in combination with chitosan (QUI+TCP) groups. Tomographic evaluation showed no statistically significant difference among groups; however radiopacity was higher in the treated groups. Comparative descriptive histological evaluation found that treatment groups stimulated a more pronounced tissue repair reaction and accelerated bone repair. Morphometric analysis showed that treatment groups presented statistically higher bone formation compared with the control group.

Preclinical therapies to prevent or treat fracture non-union: A systematic review

“…The objective of approximately half of all studies was to promote or accelerate healing of a bony defect (103/197, 52%) or treat non-union (93/103, 47%), with further information available in S3 Table . The majority of therapies (179/204 (88%)) were only evaluated once, while five interventions (chitosan [ 18 – 23 ], adipose stromal cells [ 24 – 27 ], erythropoietin [ 28 – 31 ], vascular endothelial growth factor [ 32 – 35 ] and SDF-1 [ 36 – 38 ]) were investigated by multiple studies ( Table 3 ). Chitosan as a single therapy was evaluated by six studies: four of these found significantly greater bone formation in the intervention group compared to control [ 18 , 20 – 22 ], with further detail in Table 3 .…”

TCP-Fluorapatite Composite Scaffolds: Mechanical Characterization and In Vitro/In Vivo Testing

“…Calcium phosphate ceramics have been extensively used to produce porous scaffolds due to their bone-like chemical composition as well as excellent biological properties, including biocompatibility and osteoconductivity [8][9][10][11]. The use of these bioceramics was always restricted because of its fragility and the weak rupture resistance [12].…”

Research on Improving the Manufacturing of Titanium Medical Implants, by SLM

“…In vivo tests prove the fact that the treat surfaces can generate a better and faster osseointegration, in comparison with the even surfaces. From a clinical point of view, several studies have reported that on a long term the failure rate is lower in the case of the rough implants because they ensure the adhesion, the osteoblast cells proliferation and differentiation at the metal-bone interface [7,8,9,10,11] .…”