Use of chemiluminescence analysis to determine activity of hepatic antichalone and chalone

Пашков, А. Н.; Romanov, Yu. A.

doi:10.1007/bf00839942

Cited by 2 publications

(2 citation statements)

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“…The CAS has emerged as a complex of antioxidant defense from free radicals excess; its components are characterized by potent antiradical activity [8]. This characteristic underlies the definition of biological activities of hepatic chalone and antichalone preparations.…”

Section: Methodsmentioning

confidence: 99%

Effect of epiphysectomy on the circadian dynamics of antiradical activity of chalone-antichalone system of albino rat liver

et al. 2008

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Antiradical activity of the chalone-antichalone system in the liver of albino rats is characterized by circadian rhythms. The production of tissue regulators of proliferation is synchronized with the 24-h light/darkness cycle. Active phase of the rhythm of antiradical activity falls on night hours. Epiphysectomy caused disappearance of the circadian rhythm of activity of the liver chalone-antichalone system in albino rat.

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Section: Methodsmentioning

confidence: 99%

Effect of epiphysectomy on the circadian dynamics of antiradical activity of chalone-antichalone system of albino rat liver

et al. 2008

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“…The preparations were added in a proportion of one dose per 10 ml. The dose was calculated on the basis of the antioxidant properties of chalone and antichalone discovered by A. N. Pashkov [4]. The amount of preparation inducing 50% quenching of chemiluminescence was taken as one dose.…”

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confidence: 99%

Effect of chalone and antichalone on free radical processes in rat liver

Пашков

Maslov

1998

Bull Exp Biol Med

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Effect of the proliferative regulators chalone and antichalone on the free radical system is studied on isolated and perfused rat liver under normal conditions and after ammonium chloride load (5 mM). It is shown that chalone suppressed both lipid peroxidation and antiradical system, while antichalone exhibits opposite effects on these processes. Ammonium chloride abolishes the effect of antichalone and does not change the effect produced by chalone. A hypothesis is proposed that specific function of the organ predominates over its response to local regulatory stimuli.Key Words: chalone; antichalone; isolated liver; lipid peroxidation; antiradical activityThe majority of studies in the field of regulation of cell homeostasis are aimed at investigating final effects of regulatory factors and usually employ morphological methods. Such an approach ignores cell processes which occur after the regulatory stimulus reaches the cell to the appearance of morphological changes triggered by this signal. These processes occurring during the lag-period prepare the target cell to competent response to the action of growth factors. MATERIALS AND METHODSIsolated livers from random-bred albino rats of both sexes (221+3 g) were used in the experiments. The animals were sacrificed in the morning after 24-h fasting. The liver of ketamine-narcotized and heparinized rat was connected via v.portae to a perfusion system as descnSed previously [2]. Then it was isolated from ligaments, removed from the abdominal cavity, and placed into a thermoeontrolled perfusion chamber. At the first stage the liver was perfused for 30 rain with Hank's medium (37~ pH 7.45, 5 ml/min) for stabilization of metabolic processes. During the next 30 rain the liver was perfused under the same conditions with experimental solutions containing ehalone, antiDepartment of Medical Biology and Genetics, N. N. Burdenko State Medical Academy, Voronezh chalone, 5 mM NH~C1, or a combination of growth factor with NH4CI. The preparations were added in a proportion of one dose per 10 ml. The dose was calculated on the basis of the antioxidant properties of chalone and antichalone discovered by A. N. Pashkov [4]. The amount of preparation inducing 50% quenching of chemiluminescence was taken as one dose.After the end of perfusion, the liver was frozen in liquid nitrogen and then homogenized in physiological saline containing Triton X-100 and centrifuged for 10 min at 15.000 g min. The contents of protein (by the method of Lowry) and malonic dialdehyde (MDA) [7] in the supernatant were measured. Antiradical activity of the supernatant was evaluated by chemiluminescence quenching on an KhLMITs-01 chemiluminometer [3].The significance of differences was evaluated by one-factor dispersion analysis. RESULTSOur results show that cell proliferation regulators specifically modulate such phylogenetically old protess as lipid peroxidation (LPO).As seen from Fig. 1, ehalone and antichalone had opposite effects on the intensity of LPO, judging from the content of MDA in isolated liver. ...

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Use of chemiluminescence analysis to determine activity of hepatic antichalone and chalone

Cited by 2 publications

References 3 publications

Effect of epiphysectomy on the circadian dynamics of antiradical activity of chalone-antichalone system of albino rat liver

Effect of epiphysectomy on the circadian dynamics of antiradical activity of chalone-antichalone system of albino rat liver

Effect of chalone and antichalone on free radical processes in rat liver

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