Use of ceric ammonium sulphate and two dyes, methyl orange and indigo carmine, in the determination of lansoprazole in pharmaceuticals

Abstract

Two spectrophotometric methods are proposed for the assay of lansoprazole (LPZ) in bulk drug and in dosage forms using ceric ammonium sulphate (CAS) and two dyes, methyl orange and indigo carmine, as reagents. The methods involve addition of a known excess of CAS to LPZ in acid medium, followed by determination of residual CAS by reacting with a fixed amount of either methyl orange, measuring the absorbance at 520 nm (method A), or indigo carmine, measuring the absorbance at 610 nm (method B). In both methods, the amount of CAS reacted corresponds to the amount of LPZ and the measured absorbance was found to increase linearly with the concentration of LPZ, which is corroborated by the correlation coefficients of 0.9979 and 0.9954 for methods A and B, respectively. The systems obey Beer's law for 0.5-7.0 mg mL -1 and 0.25-3.0 mg mL -1 for methods A and B, respectively. The apparent molar absorptivities were calculated to be 3.0 x 10 4 and 4.4 x 10 4 L mol -1 cm -1 for methods A and B, respectively. The limits of detection (LOD) and quantification (LOQ) were calculated to be 0.08 and 0.25 mg mL -1 for method A, and 0.09 and 0.27 mg mL -1 for method B, respectively. The intra-day and inter-day precision and accuracy of the methods were evaluated according to the current ICH guidelines. Both methods were of comparable accuracy (e r £ 2 %). Also, both methods are equally precise as shown by the relative standard deviation values < 1.5%. No interference was observed from common pharmaceutical adjuvants. The accuracy of the methods was further ascertained by performing recovery studies using the standard addition method. The methods were successfully applied to the assay of LPZ in capsule preparations and the results were statistically compared with those of the literature UV-spectrophotometric method by applying Student's t-test and F-test.

Kinetics and Mechanism of Cerium(IV) Oxidation of Fosfomycin Disodium Salt: An Antibiotic Drug in Acid Perchlorate Solutions

“…3.58 298 6.05 308 10.2 318 16.5 Ea (k J mol ¡1 ) Fosfomycin Disodium Salt 1141 study of hydrolysis of cerium(IV) indicates [7] Ce(OH) 3C is the predominant species of cerium(IV) in aqueous perchloric acid. Hence, Ce(OH) 3C is considered as the active species of oxidant.…”

“…The oxidation reactions of some compounds are very rapid when perchloric acid (HClO 4 ) medium is maintained. [5][6][7][8] This is attributed to the highest oxidation potential of cerium(IV) in HClO 4 (E o D 1.75V) as compared to that of cerium(IV) in H 2 SO 4 (E o D 1.44), HNO 3 (E o D 1.61V) or HCl (E o D1.28V). [9] In sulfuric acid and sulfate media, cerium(IV) forms several sulfato complexes.…”

Titrimetric and Spectrophotometric Assay of Ganciclovir in Pharmaceuticals Using Cerium(IV) Sulphate as the Oxidimetric Agent

“…Redox reactions always preferred highly acidic conditions. The oxidation reactions of some compounds are very rapid when perchloric acid (HClO 4 ) medium is maintained [26][27][28][29]. This is attributed to the highest oxidation potential of cerium(IV) in HClO 4 (E o = 1.75 V) as compared to that of cerium(IV) in H 2 SO 4 (E o = 1.44 V), HNO 3 (E o = 1.61 V) or HCl (E o = 1.28 V) [30].…”

Cerimetric determination of simvastatin in pharmaceuticals based on redox and complex formation reactions

“…A number of oxidisable pharmaceutical substances have earlier been assayed using cerium (IV) sulphate by several workers [32][33][34][35][36][37][38][39][40][41][42][43][44][45] based on several reaction schemes. The present methods entail adding a known excess of cerium (IV) sulphate to SMT in acid medium followed by the determination of unreacted oxidant by two reaction schemes involving the use of ferroin, and iron (II) and thiocyanate as reagents.…”