Use of Capillary Zone Electrophoresis for Differentiating Excessive from Moderate Alcohol Consumption

Legros, F.; Nuyens, Vincent; Baudoux, M.; Boudjeltia, Karim Zouaoui; Ruelle, Jean-Louis; Colicis, Jean; Cantraine, Francis; Henry, J. P.

doi:10.1373/49.3.440

Cited by 52 publications

(46 citation statements)

References 35 publications

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“…Normal asialotransferrin was defined as a level of 0%, because detectable values enable the identification of chronic and abusive alcohol consumption. 8 We examined the relation between alcohol consumption and CDT and asialotransferrin. We did not exclude participants with high CDT who reported no alcohol consumption, because the specificity of CDT is 92% for alcohol consumption.…”

Section: Methodsmentioning

confidence: 99%

“…We did not exclude participants with high CDT who reported no alcohol consumption, because the specificity of CDT is 92% for alcohol consumption. 8 According to their smoking histories, participants were classified as never, current, or former smokers (if they had stopped smoking). Blood pressure and heart rate were measured by trained field investigators, 3 times in the left arm in the seated position, after Ն10 minutes of rest, using an Omron HEM-907 automated oscillometric sphygmomanometer (Omron Matsusaka Company, Ltd., Kyoto, Japan), with an appropriately sized cuff.…”

Section: Methodsmentioning

confidence: 99%

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Alcohol Drinking and Cardiovascular Risk in a Population With High Mean Alcohol Consumption

Foerster

Marques‐Vidal

Gmel

et al. 2009

The American Journal of Cardiology

107

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Moderate alcohol consumption has been associated with lower coronary artery disease (CAD) risk. However, data on the CAD risk associated with high alcohol consumption are conflicting. The aim of this study was to examine the impact of heavier drinking on 10-year CAD risk in a population with high mean alcohol consumption. In a population-based study of 5,769 adults (aged 35 to 75 years) without cardiovascular disease in Switzerland, 1-week alcohol consumption was categorized as 0, 1 to 6, 7 to 13, 14 to 20, 21 to 27, 28 to 34, and >35 drinks/week or as nondrinkers (0 drinks/week), moderate (1 to 13 drinks/ week), high (14 to 34 drinks/week), and very high (>35 drinks/week). Blood pressure and lipids were measured, and 10-year CAD risk was calculated according to the Framingham risk score. Seventy-three percent (n ‫؍‬ 4,214) of the participants consumed alcohol; 16% (n ‫؍‬ 909) were high drinkers and 2% (n ‫؍‬ 119) very high drinkers. In multivariate analysis, increasing alcohol consumption was associated with higher high-density lipoprotein cholesterol (from a mean ؎ SE of Increasing wine consumption was more related to high-density lipoprotein cholesterol levels, whereas beer and spirits were related to increased triglyceride levels. In conclusion, as measured by 10-year CAD risk, the protective effect of alcohol consumption disappears in very high drinkers, because the beneficial increase in high-density lipoprotein cholesterol is offset by the increases in blood pressure levels.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Alcohol Drinking and Cardiovascular Risk in a Population With High Mean Alcohol Consumption

Foerster

Marques‐Vidal

Gmel

et al. 2009

The American Journal of Cardiology

107

View full text Add to dashboard Cite

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“…These results contrast with previous work suggesting that asialo-Tf (CZE) was highly efficient in identifying patients reporting a mean alcohol consumption Ͼ50 g/day (AU-ROC ϭ 0.91) (1 ). Two factors might help to explain the differences observed between that earlier study and the present one: In the earlier study, participants were older, consumed more alcohol, and were more likely to have altered hepatic function related to alcohol (1,8 ). These conditions apparently increase the sensitivity of CDT (10 ).…”

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confidence: 59%

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mentioning

confidence: 99%

“…These conditions apparently increase the sensitivity of CDT (10 ). The other factor, as suggested by Legros et al (8 ), was that in the earlier study (1 ), the performance of the biological markers was amplified because the participants were compared after exclusion of those who were abstinent or alcohol dependent and those who reported drinking 30 -50 g of ethanol/day. These findings suggest that the superiority of asialo-Tf (CZE) over CDT (TIA) is evident only in individuals who consume excessive amounts of alcohol, such as very heavy drinkers, who are predominantly alcohol dependent.…”

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confidence: 99%

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Carbohydrate-Deficient Transferrin Measured by Capillary Zone Electrophoresis and by Turbidimetric Immunoassay for Identification of Young Heavy Drinkers

Daeppen¹,

Anex²,

Favrat³

et al. 2005

Clinical Chemistry

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Carbohydrate-deficient transferrin (CDT) measured by capillary zone electrophoresis (CZE), particularly asialotransferrin (Tf), is purported to better differentiate between excessive and moderate drinkers than does CDT measured by turbidimetric immunoassay (TIA) (1,2 ). The use of biological markers such as CDT is of particular interest for identifying young heavy drinkers because other clinical signs of heavy drinking are generally absent and heavy drinking is a leading cause of morbidity and mortality in this age group (3, 4 ). Several authors have shown interest in the ability of CDT to identify nondependent heavy drinkers (5, 6 ); we therefore describe here the performance of CZE measurements of asialo-and disialo-Tf and TIA analysis of CDT in a large community sample of 19-year-old men, of whom 21% were heavy drinkers.From a sample of 1018 men attending a mandatory 1-day army recruitment process for all Swiss males at age 19 years, 1004 (98.6%) agreed to complete a research questionnaire. Of these, 581 young men (57.9%) consented to give blood for the measurement of asialo-Tf (CZE), disialo-Tf (CZE), and CDT (TIA). The Ethics Committee of the Lausanne University Medical School approved the study protocol. Volunteers were compensated for participation in the study.Volunters gave written informed consent and then completed an instrument entitled "Health and Lifestyle Questionnaire", which included questions assessing the typical quantity and frequency of alcohol consumption during the 12 months preceding the survey and the frequency of drunkenness over the last 30 days. One drink was defined as a 250-mL can or bottle of beer, a 120-mL glass of wine, or a 40-mL shot of liquor straight or in a mixed drink, and corresponded to ϳ12 g of pure ethanol.A study investigator was present during administration of the questionnaire to verify that participants answered all items. Serum samples were obtained by centrifugation of peripheral blood collected in 10-mL tubes. Samples were stored at Ϫ20°C before analysis.Total CDT was measured by anion-exchange chromatography and TIA with the Axis-Shield CDT (TIA) reagent set (7 ). To separate and measure Tf isoforms, we used a previously described and validated CZE method (8, 9 ) with the Ceofix CDT reagent set (Analis) on a Hewlett Packard (HP) 3D-CE instrument. The CZE conditions are described in Table 1 of the Data Supplement that accompanies the online version of this Technical Brief at http:// www.clinchem.org/content/vol51/issue6/. CZE electropherograms showing the serum Tf profiles for a heavy drinker before and after addition of anti-Tf polyclonal antibody to the serum are shown in Fig. 1 of the online Data Supplement, and CZE electropherograms showing the Tf profiles of a teetotaler and of 2 heavy drinkers are shown in Fig. 2 of the online Data Supplement.Peaks representing the different Tf isoforms were quantified as the amounts of the asialo-, disialo-, trisialo-, tetrasialo-, pentasialo-, and hexasialo-Tf (CZE) as a percentage of the total Tf content, in terms of va...

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Capillary zone electrophoresis with a dynamic double coating for analysis of carbohydrate‐deficient transferrin in human serum: Impact of resolution between disialo‐ and trisialotransferrin on reference limits

Lanz

Thormann

2003

Electrophoresis

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Capillary electrophoresis with a dynamic double coating formed by charged polymeric reagents represents a very effective tool for the separation of iron-saturated transferrin (Tf) isoforms and thus the determination of carbohydrate-deficient transferrin (CDT) in human serum. The resolution between di- and trisialo-Tf is dependent on the applied voltage and capillary temperature. With a 50 microm inside diameter (ID) capillary of about 60 cm total length mounted into the P/ACE MDQ, 28 kV and 40 degrees C, the resolution of the two Tf isoforms is shown to be between 1.0 and 1.4, whereas with reduced voltage and/or temperature, increased resolution at the expense of elongated run times is observed. Best data with complete resolution (Rs > or = 1.4) are obtained at 20 kV and 30 degrees C. For the determination of CDT in serum, incomplete separation of di- and trisialo-Tf is demonstrated to have an impact on the reference limits. Analysis of the sera of 54 healthy individuals with no or moderate alcohol consumption and using valley-to-valley peak integration, the upper (lower) reference limits for CDT in relation to total Tf at the two power levels are 1.33 (0.52) and 1.57 (0.81)%, respectively, representing intervals that are significantly different (P < 0.001). Furthermore, the reference intervals are shown to be strongly dependent on the peak integration approach used. Valley-to-valley peak integration should only be employed for conditions with complete resolution between disialo- and trisialo-Tf.

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Use of Capillary Zone Electrophoresis for Differentiating Excessive from Moderate Alcohol Consumption

Cited by 52 publications

References 35 publications

Alcohol Drinking and Cardiovascular Risk in a Population With High Mean Alcohol Consumption

Alcohol Drinking and Cardiovascular Risk in a Population With High Mean Alcohol Consumption

Carbohydrate-Deficient Transferrin Measured by Capillary Zone Electrophoresis and by Turbidimetric Immunoassay for Identification of Young Heavy Drinkers

Capillary zone electrophoresis with a dynamic double coating for analysis of carbohydrate‐deficient transferrin in human serum: Impact of resolution between disialo‐ and trisialotransferrin on reference limits

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