Use of calcium phosphate- silver nanoparticles in chitosan coatings on titanium and for drug delivery

Gillen, Gonzales; Joel, Bumgardner; Jegdish, Babu; Jessica, Jennings; Warren, Haggard; Adhikari, H.; Diego, Pulgarin; Timothy, Martin; Vishnu, M.C.; Sanjay, Mishra

doi:10.3389/conf.fbioe.2016.01.00396

Cited by 1 publication

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“…Magnesium has been shown to improve regeneration in full thickness skin wounds and may be attributed to the promotion of vascularization [ 34 , 35 , 36 ]. A protocol established by Zhou et al [ 37 ] was selected as the method for MgP production due to our previous success using a similar synthesis method for calcium phosphate-silver nanoparticles incorporation into chitosan coatings [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Magnesium-Phosphate Particle Incorporation into Co-Electrospun Chitosan-Elastin Membranes for Skin Wound Healing

et al. 2022

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Major challenges facing clinicians treating burn wounds are the lack of integration of treatment to wound, inadequate mechanical properties of treatments, and high infection rates which ultimately lead to poor wound resolution. Electrospun chitosan membranes (ESCM) are gaining popularity for use in tissue engineering applications due to their drug loading ability, biocompatibility, biomimetic fibrous structure, and antimicrobial characteristics. This work aims to modify ESCMs for improved performance in burn wound applications by incorporating elastin and magnesium-phosphate particles (MgP) to improve mechanical and bioactive properties. The following ESCMs were made to evaluate the individual components’ effects; (C: chitosan, CE: chitosan-elastin, CMg: chitosan-MgP, and CEMg: chitosan-elastin-MgP). Membrane properties analyzed were fiber size and structure, hydrophilic properties, elastin incorporation, MgP incorporation and in vitro release, mechanical properties, degradation profiles, and in vitro cytocompatibility with NIH3T3 fibroblasts. The addition of both elastin and MgP increased the average fiber diameter of CE (~400 nm), CMg (~360 nm), and CEMg (565 nm) compared to C (255 nm). Water contact angle analysis showed elastin incorporated membranes (CE and CEMg) had increased hydrophilicity (~50°) compared to the other groups (C and CMg, ~110°). The results from the degradation study showed mass retention of ~50% for C and CMg groups, compared to ~ 30% seen in CE and CEMg after 4 weeks in a lysozyme/PBS solution. CMg and CEMg exhibited burst-release behavior of ~6 µg/ml or 0.25 mM magnesium within 72 h. In vitro analysis with NIH3T3 fibroblasts showed CE and CEMg groups had superior cytocompatibility compared to C and CMg. This work has demonstrated the successful incorporation of elastin and MgP into ESCMs and allows for future studies on burn wound applications.

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