Use of calcium hydroxyapatite and growth factors in endodontic therapy

Abstract

Background / Aim. Hydroxyapatite (HAp) is one of the most commonly used calcium phosphate bioceramics with osteoconductive properties. Growth factors (ТGF-β) are capable of directly inducing morphological and functional differentiation of neodontoblasts. The aim of this paper was to investigate the effectiveness of biomaterials in combination with growth factors in the creation of new dentine and obturation of the root canal apex in the teeth of our experimental model. Methods. Rodent (rabbit) teeth were used as the experimental animal model. After pulp removal with a pulp extirpator in vital pulpectomy, the biomaterial was applied using a Lentulo spiral in the apex portion at the level of the physiological foramen apicale. The experiment was performed in general anesthesia. Animals were kept alive for 3, 6 and 12 months. The extraxted teeth were prepared for scanning electron microscopy (SEM) observation. Results. Using SEM, it was found that the number of teeth with newly created dentine and apex canal obturation was greater 12 months after the treatment. Conclusion. In our experimental groups, apex obturation of the dental root canal with newly created dentine took place.