Use of C57BL/6N mice on the variety of immunological researches

Abstract

Inbred mice are an essential animal strain for research as they can improve the reproducibility and reliability of study results. The establishment of new inbred lines is continuing, and new inbred lines are being used in many research fields. C57BL/6 is a mouse laboratory animal that has been developed and used as an inbred strain since early stage of mouse strain development, and, in the 1950s, C57BL/6 was separated into substrains by the Jackson Laboratory (C57BL/6J) and the National Institutes of Health (C57BL/6N). C57BL/6 mice have been used in immunology and antitumor activity studies since the early strain development stage. After the mouse genome was fully described, C57BL/6 mice use in many areas of research has expanded. In particular, immunological characteristics such as those related to cell-mediated immunity and NK cell activity are relatively higher in C57BL/6 mice than in other mice. The C57BL/6NKorl is a stock of C57BL/6N established as part of a localization of experimental animal strategy of the Korean Food and Drug Administration. Based on analysis of single nucleotide polymorphisms (SNPs), C57BL/6NKorl is considered a genetically distinct inbred stock from other C57BL/6N. Various research efforts have been made to describe the characteristics and increase knowledge of the characteristics of C57BL/6Nkorl. The results obtained through these efforts are expected to increase the utilization of C57BL/6Nkorl as a domestic laboratory animal resource and to enhance the reliability of mouse based studies.

Overview of inactivating mutations in the protein-coding genome of the mouse reference strain C57BL/6J

“…Obviously, the closely related mouse strain C57BL/6NJ shares most of the sequence-specific variation that we found in C57BL/6J, but not all of them. Some of these may also help to explain the subtle differences between C57BL/6J and C57BL/6NJ in phenotypes, as often encountered by researchers (24).…”

Compensatory role of C3 convertase on the strain difference for C3 protein expression in FVB/N, C3H/HeN and C57BL/6N mice

“…Inbred mice have long been used in various immunological researches, including cell-mediated immunity, allergic response and anti-tumor mechanism, due to their reliability, reproducibility and scope of improvement of animal-based experiments [12]. As immunological researches involving inbred strains increases, strain differences of mice on the immunological response has received growing attention [13].…”

ObLiGaRe doxycycline Inducible (ODIn) Cas9 system driving pre-clinical drug discovery, from design to cancer treatment

“…When using Cas9 mediated gene editing to generate genetically engineered models, constitutive or leaky expression of Cas9 can have a negative impact on the utility of the model. Since short induced expression of Cas9 is important to limit off target gene editing [28, 29], our aim was to create a sensitive but tightly regulated all-in-one inducible Cas9 system that could be used in a variety of genomic backgrounds of human and mouse origins as well as generating an inducible mouse.…”