Use of Bromelin to Demonstrate Erythrocyte Antibodies.

Pirofsky, Bernard; Mangum, Melvin E.

doi:10.3181/00379727-101-24828

Cited by 57 publications

(28 citation statements)

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“…A number of modifications to the one-stage test have been reported in order to improve sensitivity, including layer ing [Boorman et al, 1977] and phased addition of enzyme and serum to red cells [Pirofsky and Mangum, 1959], These modifications are primarily aimed at reducing immunoglobulin digestion by enzyme and involve some separation of the enzyme treatment of the red cells from the interaction of the red cells with serum antibodies. In separating the two phases, each may also be performed closer to its respective optimal pH than can be achieved in a mixing technique.…”

Section: Discussionmentioning

confidence: 99%

The pH Optima for Papain and Bromelain Treatment of Red Cells

Scott¹,

Johnson²,

Phillips³

1987

Vox Sanguinis

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The action of papain and bromelain, prepared over a pH range from 4.6 to 8.6, was evaluated for the ability to render red cells agglutinable by five incomplete antibodies of differing blood group specificities using a two-stage technique. The optimal pH for treatment of red cells by activated papain or bromelain was between 5.4 and 5.8. Above this pH range, a fall in serological sensitivity was apparent which was much more pronounced with papain than with bromelain. The optimal pH for enzyme treatment of red cells can be achieved in two-stage techniques, but not in one-stage techniques due to the buffering effect of serum proteins.

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Section: Discussionmentioning

confidence: 99%

The pH Optima for Papain and Bromelain Treatment of Red Cells

Scott¹,

Johnson²,

Phillips³

1987

Vox Sanguinis

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“…The improve ment in performance may be due to the use of sequential addition of enzyme and serum to cells, a higher serum to red cell volume ration, and centrifugation. The use of sequential addition of enzyme and serum to red cells has been suggested by other workers as a more sensitive variation of the one-stage mix technique [Pirofsky and Mangum, 1959;Polak, 1965;Odell et ah, 1983;Cullen and Knight, 1984], The use of antipain represents a fur ther improvement on this type of technique.…”

Section: Comparison Of Techniquesmentioning

confidence: 99%

Sensitive Two-Stage Papain Technique without Cell Washing

Scott¹,

Phillips²

1987

Vox Sanguinis

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A two-stage papain technique is described in which cell washing after papain treatment is replaced by the addition of a specific papain inhibitor. This technique permits optimal enzyme treatment of red cells while digestion of immunoglobulin following the addition of serum is avoided. The technique therefore combines the design and consequent sensitivity advantage of two-stage tests with the convenience of one-stage tests, rendering it suitable for use in compatibility testing.

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“…Detection tests were performed by a modified method of Pirofsky and Mangum [3]. One drop of bromelin solution, one drop of a 2.5% suspension of RBCs in saline and two drops of serum were mixed, incubated at 37 °C for 15 min, centrifuged 1 min at 1,000 rpm and inspected for macroscopic agglutination.…”

Section: Determination Of Proteinmentioning

confidence: 99%