Use of bovine oocytes to evaluate in vitro fertilizing capacity of equine sperm

Campos-Chillòn, L.F.; Barceló-Fimbres, M.; Carnevale, E.M.; Seidel, G.E.

doi:10.1016/j.theriogenology.2007.05.036

Cited by 3 publications

(4 citation statements)

References 2 publications

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“…Likewise, Landim-Alvarenga et al (2001) obtained similar penetration rates (13.3%) when treating stallion sperm samples with dilauroylphosphatidylcholine (PC-12) or the calcium ionophore A23187. Actually, other authors have tried to capacitate stallion spermatozoa using other different compounds and protocols (Campos-Chillòn et al, 2007;Choi et al, 2002;Klewitz et al, 2010;McPartlin et al, 2006McPartlin et al, , 2009Spizziri et al, 2010;Wilhelm et al, 1996) however the results are poor and not repeatable (reviewed by Hinrichs, 2013). Higher than our results and of great interest were the results obtained by Taberner et al (2010) using the 0.1 M ionomycin treatment to capacitate frozen-thawed donkey spermatozoa (85.4%).…”

Section: Discussionmentioning

confidence: 44%

“…Many authors have tried to capacitate stallion sperm (Campos-Chillòn et al, 2007;Choi et al, 2003;Klewitz et al, 2010;McPartlin et al, 2006McPartlin et al, , 2009Spizziri et al, 2010;Wilhelm et al, 1996) but the results have always been poor and unrepeatable (reviewed by Hinrichs, 2013). However, Taberner et al (2010) achieved oocyte penetration rates of 85.4% after capacitating frozen-thawed donkey spermatozoa with 0.1 M ionomycin.…”

Section: Introductionmentioning

confidence: 99%

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Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

Morató

Soares

Orero

et al. 2013

Animal Reproduction Science

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Section: Discussionmentioning

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

Morató

Soares

Orero

et al. 2013

Animal Reproduction Science

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“…In publications, there is evidence of their in vitro fertilization using semen of gaur [5], antelope [6], horse [7], sheep [8], and donkey [9]. Сlose relationship between the species, the lack of specific antigens against wisent spermatozoa in the pellucid zone (PZ) of cow oocytes [10], and the previously described possibility to obtain a hybrid offspring by crossing [11] are the prerequisites for extracorpo-…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

“…Domestic cattle (Bos taurus) is the leader in the efficient and large-scale obtaining of embryos in vitro. There is evidence of extracorporeal fertilization of bovine oocytes by the semen of different species [6][7][8][9], including closely related ones: gaur (Bos gaurus), banteng (B. javanicus) [5], yak (B. grunniens) [5,18], African (Syncerus caffer caffer) and Asian (Bubalus bubalis) buffalo [17,19], and American bison (Bison bison) [23].…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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A b s t r a c t European bison or wisent (Bison bonasus)is a rare species that is endangered. The technology for producing in vitro embryos using oocytes of cows and wisent semen can be an effective tool for conservation of genetic resources of this species and their rational use in basic research on the physiology of development and to create the new animals' types. However, the methods for production of in vitro embryos using the above-mentioned germ cells are currently not described in the literature. In this work, we first attempted to obtain the hybrid cow-wisent embryos in vitro using the IVM/IVF/IVC protocol, developed for domestic cattle (Bos taurus). Frozen epididymal sperm derived from wisent was thawed and prepared by the swim-up procedure. Matured oocytes were coincubated during 18 h together with prepared sperm in Fert-Talp medium, which was replaced then by embrional medium where the embryos were cultured until the blastocyst state. Additionally, for positive control, a part of matured bovine oocytes were fertilized with frozen/thawed ejaculated bull sperm. Fertilizing capacity of male cells of both species was assessed according to the nature of spermatozoa-egg interactions, as well as the capacity of fertilized bovine oocytes to subsequent embryonic development. It was found that the rate of oocytes with sing polyspermy was higher for fertilization with wisent sperm comparing to allogenic insemination (21.6 vs. 8.5 %, P < 0.05). There were no differences between two types of fertilizations nether for the sperm penetration rate (90.0±0.3 vs. 93.3±1.7 % for bull and wisent, respectively), nor for the rate of normal fertilization (78.3±1.7 % vs. 73.2±2.3 %). The similar cleavage and blastocyst formation rates were observed for two fertilization types. Our data indicate the similarity in the mechanisms of oocyte activation and embryonic development in cow and wisent females. In addition, it is obvious that IVM/IVF/IVP protocol, in general, allows in vitro producing cattle-wisent hybrid embryos, however, high levels of polyspermy observed in heterogeneous fertilization, indicates the necessity to adjust the method.Keywords: cattle oocytes, the European bison sperm, fertilization in vitro, hybrid embryos European bison, or wisent, (Bison bonasus) is a rare endangered species. Current European bison population is strongly inbred. All modern wisents come from 12 individuals kept in the zoos and reserves in the early XX century [1]. Low genetic variability is one of the main threats to the long-term maintenance of the species. Assisted reproductive technology such as production of in vitro embryos using oocytes of domestic cattle and wisent semen will not only help to maintain the existing genetic resources of the species, but also to involve this species in basic research on the physiology of development and use in creations of new bred forms [2][3][4].Cow oocytes are a universal object for interspecific hybridization. In publications, there is evidence of their in vitro fertilization using semen of gaur...

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Ability of Catalonian donkey sperm to penetrate zona pellucida-free bovine oocytes matured in vitro

Taberner¹,

Morató²,

Mogas³

et al. 2010

Animal Reproduction Science

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Use of bovine oocytes to evaluate in vitro fertilizing capacity of equine sperm

Cited by 3 publications

References 2 publications

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Ability of Catalonian donkey sperm to penetrate zona pellucida-free bovine oocytes matured in vitro

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