Use of Bone-Marrow Culture in Prediction of Acute Leukaemic Transformation in Preleukaemia

The growth of myelodysplastic bone marrow in long-term cultures

“…However, we found a similar incidence of colonies (although in some cases increased clusters) in the RAEB patients (including one case in transformation) and in patients with RA or PASA. This is in agreement with some reports (Milner et al, 1977;Francis et al, 1983).…”

Overexpression of tumor necrosis factor (TNF)-α and interferon (IFN)-γ by bone marrow cells from patients with myelodysplastic syndromes

“…7,8,12,15 Ferritin, 28 prostaglandin E2 (PGE 2 ), and lactoferrin 26 induce down-regulation of hematopoiesis by inhibiting the production of macrophage-derived granulomonopoietic enhancing activity. Bone marrow cells from MDS patients are known to have unbalanced growth characteristics [29][30][31][32] but the underlying mechanisms causing them are still unclear. Recently, an increased frequency of apoptosis was observed in bone marrow cells of MDS patients.…”

“…Some authors point to a correlation of disturbed growth of CFU-GM with abnormal production of CFS in MDS, 29,30 while others propose that leukemia-associated inhibitory activity contributes to the development of the disease processes in MDS. 31,32,35 Among several substances which have inhibitory effects on hematopoiesis, increased production of ferritin and PGE 2 have been found in bone marrow cultured cells from MDS patients.…”

Localization of Fas and Fas ligand in bone marrow cells demonstrating myelodysplasia

“…Bone marrow cells from MDS patients are known to have unbalanced growth characteristics [17][18][19][20] but the underlying mechanisms causing them are still unclear. A high rate of apoptosis explains the ineffective hematopoiesis observed in MDS.…”