Use of blood collected onto and dried on filter paper for diagnosing pregnancy in cattle

Sun, Dong; Cho, Yong-Il; Comyn, P; Yoon, Kyoung‐Jin

doi:10.1016/j.tvjl.2013.09.016

Cited by 10 publications

(11 citation statements)

References 9 publications

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“…However, results from BP samples were generally one MAT titre lower than from serum samples. This finding is in accordance with results from BP samples used to detect others’ antibodies or circulating metabolites [ 31 , 32 ]. The elution step that is required to dissolve the BP sample may explain the lower titres obtained when compared to standard serum samples [ 31 ].…”

Section: Discussionsupporting

confidence: 90%

“…Serum samples with agglutination at MAT titre ≥24 were considered positive for the presence of T. gondii antibodies [ 26 , 27 , 37 ]. However, as the level of dilution of the eluate obtained after BP elution is generally not known, the dilution level is often higher in eluates than in sera and may result in lower MAT titres [ 31 , 32 ]. For this reason, we used two cut-off values for eluates: MAT titre ≥24 and MAT titre ≥12.…”

Section: Methodsmentioning

confidence: 99%

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Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

et al. 2021

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If validated beforehand, the analysis of dried blood on blotting paper (BP samples) is very useful for monitoring free-ranging animals. We aimed to validate this method for the detection of antibodies against Toxoplasma gondii in stray cats. We used the modified agglutination test (MAT) in 199 sample pairs of sera and BP samples from 54, 39, 56, and 50 cats trapped during four periods in five dairy farms. Screening was at 1:6, 1:12, and 1:24 dilutions. The cut-off value was at MAT titre ≥ 24, but MAT titre ≥ 12 was also considered for BP samples that often have a higher dilution level. Depending on the period, sample type, and cut-off value, sensitivity of the analysis of the BP sample vs. serum varied from 87.1% to 100% and specificity ranged from 72.22% to 100%. The concordance values and Kappa coefficient showed a substantial to excellent agreement between the results of the two methods, whatever the cut-off value. These findings quantifiably validate the use of MAT on BP samples for the detection of antibodies to T. gondii in stray cats, but we recommend expressing results from BP samples with several cut-off values as the MAT titres tend to be lower than those of sera.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

et al. 2021

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“…All Whatman and similar cards are manufactured as a small piece of thick cellulose filter paper with special circled zones for a sample application. As reported, animal DMS were also collected with the help of some alternative sampling devices such as Tego Card (Yoon et al 2010 ; Sun et al 2013 ), Hemaspot device (Rosypal et al 2014 ), isoCode stix (Jefferies et al 2007 ) and Nobuto cellulose strips (Table 1 ). Nobuto cellulose stripes were first used in 1966 by Japanese scientists Kenzo Nobuto to collect swine blood for serodiagnosis of toxoplasmosis (Nobuto 1966 ).…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

“…Some works described the optimization of antibodies elution time and temperature (Hopkins et al 1998 ; Chadio et al 2002 ). Similarly, to increase concentration of cattle pregnancy-associated glycoproteins (PAGs) in paper eluate Sun et al ( 2013 ) used a modified ELISA protocol including increased sample volume, longer incubation time and plate shaking during incubation. In general, there are no established standardized procedures for antibodies extraction from dried biological fluids.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

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Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

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“…Classical veterinary may use DBS method for detecting pregnancy in cows and heifers [16][17]. Smith and Burgoyne [18] reported good results of nucleic acids long-term storage on cellulose fiber matrices for further DNA analysis and pathogens detection as exemplified by blood samples taken from chicken (Gallus gallus), cow (Bos taurus), western shingleback Tiliqua rugiosa and Australian pelican (Pelecanus conspicilattus).…”

Section: Introductionmentioning

confidence: 99%

Comparative research of effectiveness of cellulose and fiberglass porous membrane carriers for bio sampling in veterinary and food industry monitoring

Gusev

Vasyukova

Zakharova

et al. 2017

AIP Conference Proceedings

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Abstract. The aim of proposed research is to study the applicability of fiberglass porous membrane materials in a new strip format for dried blood storage in food industry monitoring. A comparative analysis of cellulosic and fiberglass porous membrane materials was carried out to obtain dried samples of serum or blood and the possibility of further species-specific analysis. Blood samples of Sus scrofa were used to study the comparative effectiveness of cellulose and fiberglass porous membrane carriers for long-term biomaterial storage allowing for further DNA detection by real-time polymerase chain reaction (PCR) method. Scanning electron microscopy of various membranes -native and with blood samples -indicate a fundamental difference in the form of dried samples. Membranes based on cellulosic materials sorb the components of the biological fluid on the surface of the fibers of their structure, partially penetrating the cellulose fibers, while in the case of glass fiber membranes the components of the biological fluid dry out as films in the pores of the membrane between the structural filaments. This fundamental difference in the retention mechanisms affects the rate of dissolution of the components of dry samples and contributes to an increase in the efficiency of the desorption process of the sample before subsequent analysis. Detecting of pig DNA in every analyzed sample under the performed Real-time PCR as well as good state of the biomaterial preservation on the glass fiber membranes was clearly demonstrated. Good biomaterials preservation has been revealed on the test cards for 4 days as well as for 1 hour.

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Use of blood collected onto and dried on filter paper for diagnosing pregnancy in cattle

Cited by 10 publications

References 9 publications

Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Comparative research of effectiveness of cellulose and fiberglass porous membrane carriers for bio sampling in veterinary and food industry monitoring

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