Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae

Lindell, Shirley S.; Quinn, Patrick

doi:10.1128/jcm.1.5.440-443.1975

Cited by 10 publications

(4 citation statements)

References 7 publications

(2 reference statements)

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“…32 The number of CFUs was determined using a culture-based method because it is a simple, reliable, and rapid method for determining the presence of viable cells in a sample. 33 To collect samples, sterile absorbent paper points were inserted into the canal to absorb all contaminated canal fluid. The paper points were then placed in tubes containing 1 mL of sterile saline, which cannot support bacterial growth, in order to maintain the concentration of bacterial load within each tube, and bacteria were grown over bile esculin.…”

Section: Discussionmentioning

confidence: 99%

Microbial Evaluation Following Two Irrigation-Medication Protocols in Secondary Infection Cases

hashad¹,

Labib

Shaheen

2022

Egyptian Dental Journal

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Aim:The aim of this study was to clinically evaluate the antibacterial efficacy of two materials used as irrigants and intracanal medications (chlorohexidine, propolis) in secondary infection cases. Materials and methods:Thirty-two patients with single-rooted, single-canal teeth associated with secondary infection were randomly assigned into four groups according to the type of irrigating solution and intracanal medication used. Group 1 (2% CHX irrigation and CHX gel intracanal medication), Group 2 (2% CHX irrigation and Propolis gel intracanal medication), Group 3 (30% Propolis irrigation and CHX gel intracanal medication), and Group 4 (30% Propolis irrigation and Propolis gel intracanal medication). The first microbial sample (S1) was obtained following complete aseptic removal of the primary filling material then the second microbial sample (S2) was obtained following chemomechanical preparation with various irrigant solutions. ProTaper Universal rotary system was used up to F4, or F5 according to canal size for root canal reinstrumentation. Finally, the third microbial sample (S3) was collected after removal of the intracanal medication. After cultivating the three samples, the growing colonies were counted and recorded as colony forming units (CFU). Results:The third microbial sample after intracanal medication recorded the lowest microbial count in all groups. No statistical significant difference was recorded between the rate of reduction of S2 to S1 among the tested groups while there was statistically significant difference in microbial reduction of S3 to S2. Comparing between groups regardless the samples, there was no statistical significant differences between the groups.Conclusions: Both CHX and Propolis irrigation and intracanal medication aid in microbial reduction, particularly in cases of secondary infection.

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Section: Discussionmentioning

confidence: 99%

Microbial Evaluation Following Two Irrigation-Medication Protocols in Secondary Infection Cases

hashad¹,

Labib

Shaheen

2022

Egyptian Dental Journal

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“…After 24 h on BEM, a light inoculum, produced by touching the top of a colony with a bacteriological wire, is optimum. With an inoculum consisting of a full loopful of bacteria from solid media, such as that used by Lindell and Quinn (6), a higher positivity rate may be expected. One should also read the BEM slant as close to 18 h as possible since E. coli glucosidase is inducible.…”

Section: Discussionmentioning

confidence: 99%

Esculin hydrolysis by Enterobacteriaceae

Edberg¹,

Pittman²,

Singer³

1977

J Clin Microbiol

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Literature reports disagree concerning esculin hydrolysis in the family Enterobacteriaceae. A total of 2,490 strains of the family were investigated for esculin hydrolysis by two methods, the esculin spot test and the PathoTec incubation strip, which measures constitutive enzyme, and five growth-supporting methods, which determine both constitutive and inducible enzymes. The five growth-supporting media studied were: Vaughn-Levine, the standard esculin hydrolysis medium (P. R. Edwards and W. H. Ewing, Identification of Enterobacteriaceae, 3rd ed., 1972); Vaughn-Levine without iron; Vaughn-Levine without Andrade's indicator; and bile-esculin medium. Growth media were incubated at 35 degrees C and checked every 24 h for 120 h. On growth media, 0.3% of Escherichia coli were positive in 24 h, 34% in 48 h, and 61% in 120 h. No strains were positive on the "nongrowth" tests. It appeared that the esculin hydrolysis enzyme(s) of E. coli was inducible rather than constitutive. All esculin hydrolyzers, which yielded positive tests on "constitutive tests" and 24-h tests, were limited to the genera Klebsiella, Enterobacter, and Serratia and species of Proteus vulgaris, Proteus rettgeri, and Citrobacter diversus. When used with standardized inoculum size and incubation time, the esculin hydrolysis test is very useful for differentiation within the family Enterobacteriaceae.

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“…Wasilauskas (10) 69 0 0 ND ND ND ND Lindell and Quinn (6) 126 2 18 ND ND ND ND Edberg et al (1) 510 0 0 ND ND ND ND Edberg et al (2) 974 0 0.5 35 52 58 61 a ND, Not done. b X, Included in 48-h data.…”

Section: Methodsmentioning

confidence: 99%

Esculin hydrolysis reaction by Escherichia coli

Miskin¹,

Edberg²

1978

J Clin Microbiol

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The literature contains variable reports concerning the hydrolysis of esculin by members of the family Enterobacteriaceae and particularly Escherichia coli. We examined 113 strains of fresh clinical isolates of E. coli and assessed the ability of colonies in a population to hydrolyze esculin with and without preincubation in inducible substrates at 24, 48, and 72 h. The number of strains capable of fermenting salicin, a sugar with a beta-glucoside linkage like esculin, was studied under the same conditions. A strip test that measured the presence of the constitutive glucosidase was also performed with and without preincubation in inducible substrates. No E. coli strain was able to produce constitutive enzyme; preincubation in esculin and salicin resulted in an induction of the beta-glucosidase. The number of colonies able to hydrolyze esculin increased with time. Only those strains preincubated in esculin or salicin were able to produce a positive constitutive strip test. Because the beta-glucosidase of E. coli is inducible, one should employe, when using growth media, a light inoculum obtained by touching the top of a colony with a bacteriological wire and read the reaction between 18 and 24 h, or perform a rapid strip or spot test.

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Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae

Cited by 10 publications

References 7 publications

Microbial Evaluation Following Two Irrigation-Medication Protocols in Secondary Infection Cases

Microbial Evaluation Following Two Irrigation-Medication Protocols in Secondary Infection Cases

Esculin hydrolysis by Enterobacteriaceae

Esculin hydrolysis reaction by Escherichia coli

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