Use of basic mobile phase to improve chromatography and boost sensitivity for quantifying tetrahydrocurcumin in human plasma by LC–MS/MS

Tan, Aimin; Yanxin, Wu; Wong, Molly D.; Licollari, Albert; Bolger, Gordon T.; Fanaras, John C.; Shopp, George M.; Helson, Lawrence

doi:10.1016/j.jchromb.2016.06.010

Cited by 19 publications

(23 citation statements)

References 12 publications

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“…Owing to the wide concentration ranges and special characteristics of the analytes and the LC–MS/MS instrument employed, slightly nonlinear calibration curves were consistently obtained (Tan et. al., ; Tan et. al., ) .…”

Section: Resultsmentioning

confidence: 99%

“…al., ) . Accordingly, a quadratic calibration model with a weighting factor of 1/ x 2 was adopted to achieve better accuracy of quantification (Tan et al, ; Tan et al, ; Tan et al, ). A representative calibration curve for candesartan is: y = 5.8235 × 10 −10 x 2 + 3.0564 × 10 −4 x – 2.399 × 10 −3 , where y and x represent analyte/IS peak area ratio and analyte concentration, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous quantification of candesartan and irbesartan in rabbit eye tissues by liquid chromatography–tandem mass spectrometry

Tan

Gui

Wong

et al. 2020

Biomedical Chromatography

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Diabetic retinopathy is a major cause of vision loss in adults. Novel eye-drop formulations of candesartan and irbesartan are being developed for its cure or treatment. To support a preclinical trial in rabbits, it was critical to develop and validate a new LC-MS/MS method for simultaneous quantification of candesartan and irbesartan in rabbit eye tissues (cornea, aqueous humor, vitreous body and retina/choroid). Eye tissue samples were first homogenized in H 2 O-diluted rabbit plasma. The candesartan and irbesartan in the supernatants together with their respective internal standards (candesartan-d 4 and irbesartan-d 4 ) were extracted by solid-phase extraction. The extracted samples were injected onto a C 18 column for gradient separation. The MS detection was in the positive electrospray ionization mode using the multiple reaction monitoring transitions of m/z 441 ! 263, 445 ! 267, 429 ! 207, and 433 ! 211 for candesartan, candesartan-d 4 , irbesartan and irbesartan-d 4 , respectively. For the validated concentration ranges (2-2000 and 5-5000 ng/g for candesartan and irbesartan, respectively), the within-run and between-run accuracies (% bias) were within the range of −8.0-10.0. The percentage CV ranged from 0.6 to 7.3. There was no significant matrix interference nor matrix effect from different eye tissues and different rabbits. The validated method was successfully used in the Good Laboratory Practice (GLP) study of rabbits. K E Y W O R D S candesartan, eye tissue, irbesartan, LC-MS, rabbit

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Simultaneous quantification of candesartan and irbesartan in rabbit eye tissues by liquid chromatography–tandem mass spectrometry

Tan

Gui

Wong

et al. 2020

Biomedical Chromatography

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“…Apart from basic compounds, acids (Svahn & Björklund, ; X.‐M. Xu, Cai, Zhang, Ren, & Han, ), neutral compounds (Tan et al, ; Tan, Gu, et al, ; Hirabayashi, Sugimoto, Matsumoto, Amano, & Moriwaki, ; Rainville et al, ; Svahn & Björklund, ), and peptides (Oe et al, ) can all benefit from the use of high‐pH mobile phases. Sometimes a basic mobile phase is the only option.…”

Section: Benefits Of Using High‐ph Mobile Phasesmentioning

confidence: 99%

“…However, the compounds of interest may not be stable in a basic pH medium or not completely soluble (a basic compound can be significantly more hydrophobic at high pH than in an acidic pH medium). In this case, neutral (Tan et al, ) or acidic (Amundsen, Øiestad, Ekeberg, & Kristoffersen, ) reconstitution solutions can be used. However, it is important to make sure that the acidity of the injected sample can be adjusted to the targeted high pH of the mobile phases (Amundsen et al, ).…”

Section: Practical Considerations For Using High Ph Mobile Phasesmentioning

confidence: 99%

“…However, the extracted samples (curcumin and tetrahydrocurcumin) are not stable in a basic medium for an extended period of time (Liu, Lou, Zhao, & Fan, ). Hence, they are reconstituted in acetonitrile–H 2 O (60:40, v /v), which has the same percentage of organic modifier as the mobile phase, acetonitrile–H 2 O–NH 4 OH, 60:39.8:0.2 (v/v/v), but without the addition of ammonium hydroxide (Tan et al, ).…”

Section: Practical Considerations For Using High Ph Mobile Phasesmentioning

confidence: 99%

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Use of high‐pH (basic/alkaline) mobile phases for LC–MS or LC–MS/MS bioanalysis

Tan

Fanaras

2018

Biomedical Chromatography

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High-pH or basic/alkaline mobile phases are not commonly used in LC-MS or LC-MS/MS bioanalysis because of the deeply rooted concern with column instability and reduced detection sensitivity for basic compounds in high-pH mobile phases owing to charge neutralization. With the advancement of LC column technology and the wide recognition of the "wrong-way-round" phenomena, high-pH mobile phases are more and more used in LC-MS or LC-MS/MS bioanalysis to improve chromatographic peak shape, retention, selectivity, resolution, and detection sensitivity, not only for basic compounds, but also for many other compounds. In this article, the benefits, practical considerations, application examples and cautions for using high-pH mobile phases in LC-MS or LC-MS/MS bioanalysis are reviewed, with a focus on quantification. Furthermore, the future trends in this field are also envisaged.A total of 84 references are cited in this review. KEYWORDS alkaline mobile phase, basic mobile phase, bioanalysis, high pH, LC-MS, LC-MS/MS

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Determination and dissipation of afidopyropen and its metabolite in wheat and soil using QuEChERS–UHPLC–MS/MS

Chen

Guo²,

Liu

et al. 2018

J of Separation Science

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The dissipations of afidopyropen and its metabolite in wheat plant and soil were determined using a quick, easy, cheap, effective, rugged, and safe method with ultra-high performance liquid chromatography and tandem mass spectrometry under a field ecosystem. The limits of quantification were estimated for both target compounds as 0.001 mg/kg. The recoveries of afidopyropen and its metabolite ranged from 94 to 114% (soil), 90 to 109% (wheat seed) and 81 to 91% (wheat straw) at levels of 0.001, 0.01, 0.1, and 2.0 mg/kg with relative standard deviations ≤7%. The results of the residual dynamics experiments showed that afidopyropen dissipated rapidly in wheat plant and soil. Its metabolite initially showed a tendency of rapid increase followed by a decrease in wheat plant but could not be detected in soil. The data showed that the first + first-order model was more suitable for describing the decline of afidopyropen in wheat and soil. The half-lives of afidopyropen in wheat plant and soil were 1.65 and 1.21 days, respectively.

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Use of basic mobile phase to improve chromatography and boost sensitivity for quantifying tetrahydrocurcumin in human plasma by LC–MS/MS

Cited by 19 publications

References 12 publications

Simultaneous quantification of candesartan and irbesartan in rabbit eye tissues by liquid chromatography–tandem mass spectrometry

Simultaneous quantification of candesartan and irbesartan in rabbit eye tissues by liquid chromatography–tandem mass spectrometry

Use of high‐pH (basic/alkaline) mobile phases for LC–MS or LC–MS/MS bioanalysis

Determination and dissipation of afidopyropen and its metabolite in wheat and soil using QuEChERS–UHPLC–MS/MS

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